Effects of Extraction Temperature on Efficacy of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Aqueous Extract against Oxidative Stress

Tulsawani, Rajkumar; Sharma, Purva; Manickam, Manimaran; Koganti, Praveen; Singh, Mrinalini; Meena, Dharmendra Kumar; Negi, P. S.; Misra, Kshipra

doi:10.1615/intjmedmushrooms.2020035074

Cited by 7 publications

(5 citation statements)

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“…The results showed that the fractions of ethyl acetate, methanol, and acetonitrile inhibit the growth of both Gram-positive and Gram-negative bacteria. The TLC profile of the ethyl acetate fraction revealed a single spot with an Rf value of 0.5, like alternariol-like compounds isolated from fungi (Wei et al 2017;Tulsawani et al 2020). Trans-1,3dimethyl-cyclohexane was one of the metabolites identified in the fraction that might have biological activity.…”

Section: Discussionmentioning

confidence: 91%

Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells

SAHU,

YEERAVALLI,

DAS

et al. 2023

Nusantara Biosci

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Abstract. Sahu MK, Yeeravalli R, Das A, Jha H. 2023. Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells. Nusantara Bioscience 15: 137-142. The medicinal capabilities of plants are influenced by soil chemistry, genotype, and climate. Many biotic and abiotic factors affect soil composition. Microorganisms constituting the soil microflora indicate a mutualistic relationship with plant rhizospheric region, and they play an important role in plant secondary metabolite production, yield, and efficacy. They are the major resources for structurally unique bioactive natural metabolites. The aim of the present study was to evaluate the bioactivity of the secondary metabolite extracted from the rhizospheric fungal isolate Aspergillus carneus ABRF4) isolated from the Achanakmar Biosphere Reserve, Chhattisgarh, India. The fungal secondary metabolites were extracted using several solvents by soxhlet extraction techniques. The crude and partially purified column fractions of A. carneus ABRF4 were characterized by Gas Chromatography-Mass Spectroscopy (GC-MS), Fourier Transform Infra-Red (FTIR) spectrum, and Thin Layer Chromatography (TLC). Results showed that the acetonitrile fraction had an antimicrobial activity with the variable zone of inhibition against human pathogens such as Bacillus circulans (MTCC-7906), Bacillus subtilis (MTCC 441), Staphylococcus aureus (MTCC-96), and Ralstonia eutropha (MTCC-2487). The crude extracts and the identified secondary metabolite, trans- 1,3-dimethyl-Cyclohexane, possess different anti-proliferative activity against human tissue-specific cancer cell lines, including breast cancer (MDA-MB-231, MDA-MB-468, and MCF-7), liver cancer (HepG2), lung cancer (A-549), and prostate cancer (DU-145) suggesting a potential therapeutic application of the isolated rhizospheric fungi.

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Section: Discussionmentioning

confidence: 91%

Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells

SAHU,

YEERAVALLI,

DAS

et al. 2023

Nusantara Biosci

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“…Recent studies have indicated that several factors may be responsible for efficacy of Ganoderma . With regard to extraction temperature, a previous study found that extracts of Ganoderma with water below 100˚C exhibit high antioxidant capacity and cytoprotective effects against oxidative damage ( 42 ). In terms of molecular weight of Ganoderma polysaccharides, the association between molecular weights and biological activities of the polysaccharides have been demonstrated in several studies.…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects of a subcritical water extract of Ganoderma

Hattori¹,

Ogata²,

Yamada³

et al. 2022

Biomed Rep

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Ganoderma, a medicinal mushroom with various physiological activities, has been extensively investigated regarding its effectiveness. The aim of the present study was to examine the effects of a subcritical water extract of Ganoderma (SWEG) on the immune system. The use of subcritical water with a higher temperature and pressure than hot water allows efficient elution of components from natural products. As an evaluation of the effectiveness of SWEG, a cell proliferation and a cell differentiation test were carried out using A-6 cells, a model of hematopoietic stem cells. Furthermore, an oral administration test in mice was conducted to examine the effects of SWEG on the number and function of immune cells. As a result, SWEG was revealed to promote both self-renewal and differentiation into immune cells such as T cells and natural killer (NK) cells in experiments with A-6 cells. These results were not obtained in experiments using hot water extract of Ganoderma lucidum and Ganoderma sinense. The oral administration test in mice demonstrated that SWEG increased hematopoietic precursor cells, immature B cells, and NK cells in the bone marrow, and T cells in the thymus. In addition, SWEG enhanced the immune functions in the spleen by promoting granzyme B expression and NK cell activity. SWEG was demonstrated to be a food material that acts on HSCs and regulates immunity in vivo.

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“…Though not directly linked with NDDs, some of these compounds may work on other related systems as complementary treatments. For example, adenosine derivatives derived from Ganoderma lucidum were found to have antioxidant effects, preventing the loss of cell viability and the production of ROS after H 2 O 2 exposure [ 84 , 85 ]. Ganoderma alkaloids, such as Ganoderma amine B, arrest the MCF-7 cell cycle in the S-phase by inducing DNA fragmentation, thus reducing mitochondrial membrane potential, and have good antioxidant activity on MCF-7 cells (EC50 value is 0.27 ± 0.02 μmol/mL), which may benefit the nervous system [ 86 ].…”

Section: Potential Active Compounds From Ganoderma Lucidum ...mentioning

confidence: 99%

The Biological Activity of Ganoderma lucidum on Neurodegenerative Diseases: The Interplay between Different Active Compounds and the Pathological Hallmarks

Lian,

Yang,

Duan

et al. 2024

Molecules

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Neurodegenerative diseases represent a cluster of conditions characterized by the progressive degeneration of the structure and function of the nervous system. Despite significant advancements in understanding these diseases, therapeutic options remain limited. The medicinal mushroom Ganoderma lucidum has been recognized for its comprehensive array of bioactive compounds with anti-inflammatory and antioxidative effects, which possess potential neuroprotective properties. This literature review collates and examines the existing research on the bioactivity of active compounds and extracts from Ganoderma lucidum in modulating the pathological hallmarks of neurodegenerative diseases. The structural information and preparation processes of specific components, such as individual ganoderic acids and unique fractions of polysaccharides, are presented in detail to facilitate structure–activity relationship research and scale up the investigation of in vivo pharmacology. The mechanisms of these components against neurodegenerative diseases are discussed on multiple levels and elaborately categorized in different patterns. It is clearly presented from the patterns that most polysaccharides of Ganoderma lucidum possess neurotrophic effects, while ganoderic acids preferentially target specific pathogenic proteins as well as regulating autophagy. Further clinical trials are necessary to assess the translational potential of these components in the development of novel multi-target drugs for neurodegenerative diseases.

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Effects of Extraction Temperature on Efficacy of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Aqueous Extract against Oxidative Stress

Cited by 7 publications

References 0 publications

Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells

Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells

Immunostimulatory effects of a subcritical water extract of Ganoderma

The Biological Activity of Ganoderma lucidum on Neurodegenerative Diseases: The Interplay between Different Active Compounds and the Pathological Hallmarks

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