Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis

Abstract

Invertase is an important enzyme used in many fields especially in food industry to produce fructose syrups. The current study focused on increasing invertase production by exposing Rhodotorula glutinis to extremely low magnetic fields (ELMF; 0 and 7 mT). For this purpose, the microorganism was allowed to grow in normal magnetic field and ELMF for 72 hours at the same temperature (24 ± 2°C). The fermentation was carried out in submerged culture for 120 hours. The results showed that invertase production is strongly dependent on the growth conditions of the microorganism. Both of the different magnetic fields applied to R. glutinis increased invertase production ranged from 48%-67% when compared with the control. On the other hand, ELMF treatment increased biomass formation about 14%-28% when compared with the control. As a result, magnetic field treatment could effectively be used in the production of invertase by R. glutinis.

Low‐Frequency Magnetic Field of Appropriate Strengths Changed Secondary Metabolite Production and Na+ Concentration of Intracellular and Extracellular Monascus purpureus

“…LF‐MF has been proven to affect microbial metabolism as a physical means. After LF‐MF treatment, the metabolic rate increased by increasing the content of invertase in Rhodotorula glutinis [Canli et al, 2011]. It was found that a magnetic field strength of 20–200 μT could affect the level of reactive oxygen species, which played an important role in the accumulation of secondary metabolites in Aspergillus terreus [Cridland et al, 1999; Miranda et al, 2014].…”

Effects of extremely low frequency magnetic field on production of mannatide by α‐hemolytic Streptococcus

“…ELF‐MF can make nisin and biomass production increase three and five times [Alvarez et al, ]. It can promote ethanol [Perez et al, ] and recombinant gp41 protein production [Justo et al, ] to increase 17% and 20%, respectively, and make invertase production increase 48–67% and biomass increase 14–67% [Canli et al, ]. In our previous study, we found that it can promote citric acid production to increase more than 60%, cellulose enzyme activity increase nearly 30% [Gao et al, ], and can also make yellow pigment production increase 65.4% and red pigment increase 59.2% [Zhang et al, ].…”

Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non‐conventional yeast

“…Their strong oxidative metabolism enables the degradation of recalcitrant substrates, organochemicals and industrial wastes (Cheirsilp et al ., ; Johnson, ; Taskin, ). They are also naturally capable of bioconverting a variety of by‐products of the agrifood industry into added value primary and secondary metabolites and, based on that, have been proposed as a source of pigments and metabolites of interest in the food industry (Hernández‐Almanza et al ., ), as oil producers for biofuel application (Galafassi et al ., ; Li et al ., ; Tampitak et al ., ) and as enzyme producers (Canli et al ., ; Taskin, ). Moreover, these yeasts show antimicrobial activity against pathogenic fungi involved in postharvest diseases of fruit and vegetables (Li et al ., ; Zhang et al ., , ).…”