“…To date, a unified winter hardiness grading standard has not been established to measure alfalfa's ability to survive the winter and regenerate in spring [18] or, in other words, its performance to tolerate frost, snow accumulation, freezing, drying and other extreme winter stresses [12,19]. Currently, most studies use winter survival rate (WSR) and autumn dormancy grade [14,18] to characterize alfalfa's winter hardiness, in addition to other physiological indicators, such as malondialdehyde, soluble sugar, soluble protein and catalase [12,14,17,20,21]. One of the longstanding critiques for this approach is that the relationship between autumn dormancy and winter hardiness is not well established [19].…”