Effects of fatty acid concentration and positional specificity on maize triglyceride structure

Abstract

The effects of fatty acid concentration and positional specificity on maize triglyceride structure were evaluated from the stereospecific analyses of triglycerides from 12 genotypes. The fatty acids at each position were influenced by the fatty acid concentration in the total triglyceride except for the saturates in the 2 position. The fatty acid concentration had the greatest effect on the fatty acid composition of position 3. The existence of positional specificity was evident from the nonrandom distribution of the fatty acids among the three positions of the triglycerides. The concentration and positional specificity effects could be separated in selected genotypes and their crosses. This indicated different genetic controls for each effect.

Analyses of Vegetable Oil Triglyceride Molecular Species by Reversed Phase High Performance Liquid Chromatography

“…These oils may exhibit a preferentially or genetically controlled distribution of fatty acids (16,17). However for sunflower oil, the variation was less than an E of 4 for TGMS Downloaded by [McGill University Library] greater than 10%.…”

Stereospecific analysis of soybean triacylglycerols

“…Stereospecific plots of plant lines that differ in acyl group composition show linear relationships between the percentages of an acyl group on the sn-1, -2, and -3 positions and the percentage of that acyl group in the total TAG. This relation has been shown for several species (2)(3)(4)(5)(6). Fatemi and Hammond (4) suggested that these plots would be helpful in identifying TAG distribution mutants since such mutants would yield data points that departed from the linear plots typical of the species.…”

Triacylglycerols of the olive fruit (Olea europaea L.): characterization of mesocarp and seed triacylglycerols in different cultivars by liquid chromatography and13C NMR spectroscopy

“…More advanced research carried out in the chemistry of triacylglycerols of vegetable oils by 13 C NMR spectroscopy showed that unsaturated and carbonyl carbon resonances of acyl chains enabled the regiospecific analysis of triacylglycerols by determining the acyl chain composition of 1,3 and 2-glycerol positions, no differentiation of sn-1 and sn-3 positions being allowed. The attachment specificity of unsaturated chains at 2-position was also calculated, thus improving substantially the knowledge of molecular structure of triacylglycerols [5][6][7][8].…”

“…The attachment specificity of unsaturated chains at 2-position was also calculated, thus improving substantially the knowledge of molecular structure of triacylglycerols [5][6][7][8]. The regiospecific analysis of vegetable oils from different botanical origins was successfully performed by a new quantitative 13 C NMR methodology based on the resonances of carbonyl carbons of triacylglycerols [9].…”

“…Mesocarp and seed oils, likewise the cultivars differed significantly (P < 0.05) with regard to all the triglyceride components. 13 C NMR spectroscopy based on carbonyl carbon resonances of triglyceride acyl chains was applied to carry out the regiospecific analysis of triacylglycerols. Acyl chain composition, chain distribution among 1(3)-and 2-glycerol positions, and chain 2-positional specificity, were determined.…”