Effects of fatty acids and eicosanoid synthesis inhibitors on the growth of two human prostate cancer cell lines

Rose, David P.; Connolly, Jeanne M.

doi:10.1002/pros.2990180306

Cited by 240 publications

(216 citation statements)

References 24 publications

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“…[7][8][9] Several authors have reported reduced multiplication of malignant cultured cells incubated with n-3 fatty acids. 10,11 This effect has also been reported in leukemia cell lines. 12,13 Moreover, n-3 fatty acids and some other polyunsaturated fatty acids (PUFA) may decrease DNA synthesis and viability of cultured human lymphocytes.…”

Section: Introductionsupporting

confidence: 63%

Multiplication and death-type of leukemia cell lines exposed to very long-chain polyunsaturated fatty acids

et al. 1998

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Polyunsaturated fatty acids (PUFA) may reduce cell multiplication in cultures of normal, as well as transformed, white blood cells. We assessed the sensitivity of 14 different leukemia cell lines to PUFA by measuring cell number after 3 days of incubation. Ten of the examined cell lines were sensitive to 30, 60 and/or 120 M of arachidonic, eicosapentaenoic and docosahexaenoic acid, whereas four cell lines were resistant. The sensitivity to PUFA was not associated with any particular cell lineage, clinical origin or specific mRNA pattern of bcl-2 and c-myc. Effects on cell viability were assessed by studying cell membrane integrity, DNA fragmentation and cell morphology. The sensitive cell lines Raji and Ramos died by necrosis and apoptosis, respectively, during incubation with eicosapentaenoic acid, whereas the viability of the resistant U-698 cell line was unaffected. The effects of EPA on Raji cells, was counteracted by vitamin E, indicating that lipid peroxidation was involved. However, apoptosis induced by eicosapentaenoic acid in Ramos cells, was unaffected by vitamin E, as well as eicosanoid synthesis inhibitors. In conclusion, our results indicate that a majority of leukemia cell lines are sensitive to PUFA. This sensitivity may be caused by induction of apoptosis or necrosis by very long-chain polyunsaturated fatty acids.

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Section: Introductionsupporting

confidence: 63%

Multiplication and death-type of leukemia cell lines exposed to very long-chain polyunsaturated fatty acids

et al. 1998

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“…This hypothesis is not supported by the observation that cyclooxygenase inhibitors like indomethacin are unable to influence the growth of tumour cells in vivo (Feldman and Hilf, 1985;Abou-El-Ela et al, 1989). In addition, the effects of indomethacin on various in vitro models show that it can either stimulate or inhibit tumour cell proliferation (Buckman et al, 1991;Fulton, 1984;Rose and Connolly, 1990;Bayer et al, 1979;Hial et al, 1977). Other studies have suggested that increased lipid peroxidation may be an important cause of cytotoxicity associated with n-3 PUFAs but a definite role has not been established (Falconer et al, 1994;Lystad et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest and induction of apoptosis in pancreatic cancer cells exposed to eicosapentaenoic acid in vitro

Lai

Ross²,

Fearon³

et al. 1996

Br J Cancer

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Summary Eicosapentaenoic acid (EPA) has been shown to have an inhibitory effect on the growth of several pancreatic cancer cell lines in vitro. This study investigates the mechanism of growth inhibition and cytotoxicity of EPA on the pancreatic cancer cell line MIA PaCa-2. Cells were analysed for cell count, viability, cell cycle distribution and ultrastructural changes. There was a time-and dose-dependent decrease in cell count and viability in cultures of pancreatic cancer cells supplemented with EPA. Flow cytometric DNA anlaysis of MIA PaCa-2 cells incubated with EPA demonstrated the presence of sub G1 populations corresponding to the presence of apoptotic cells and the blockade of cell cycle progression in S-phase and G2/M-phase. The presence of apoptosis in EPA-supplemented cultures was further confirmed by DNA fragmentation and ultrastructural changes associated with apoptosis. Therefore, we conclude that EPA mediates its effect on the pancreatic cancer cell line MIA PaCa-2, at least in part, via cell cycle arrest and the induction of apoptosis.

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“…This has been supported in a human study on fish diet (rich in n-3 PUFAs) and breast cancer rates (Kaizer et al, 1989). In vitro, the inhibitory effect of PUFAs on cell proliferation has been well documented (Morisaki et al, 1982;Begin et al, 1986;Rose and Connolly, 1991;H0stmark and Lystad, 1992;Krokan et al, 1993;Mzehle et al, 1995).…”

mentioning

confidence: 84%

Differential effect of polyunsaturated fatty acids on cell proliferation during human epithelial in vitro carcinogenesis: involvement of epidermal growth factor receptor tyrosine kinase

Mollerup

Haugen²

1996

Br J Cancer

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Summary Polyunsaturated fatty acids (PUFAs) have been implicated in tumour development and have been shown to influence cell proliferation in vitro. We report here that n-3 and n-6 PUFAs at concentration > 10 gM inhibited the proliferation of a human kidney epithelial cell line (2IHKE), which has retained phenotypic characteristics of normal kidney epithelial cells. In contrast, the proliferation was stimulated by n-3 and n-6 PUFAs at concentrations <10 gM under defined growth conditions. The stimulatory effect of n-3 and n-6 PUFAs was even more profound in the presence of EGF. In human kidney epithelial cell lines reflecting different stages of transformation (1IHKE and ITHKEras), the stimulatory effect was abrogated both in the presence and absence of EGF. Saturated fatty acids did not show any stimulatory effect on cell growth. The tyrosine kinase inhibitors genistein and tyrphostin-47 inhibited EGF-induced protein tyrosine phosphorylation dose-dependently in the 2IHKE cells, and abolished the growth stimulatory effect of docosahexaenoic acid (DHA). This indicates the involvement of EGF receptor tyrosine kinase activity in the observed increase in cell proliferation.

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Effects of fatty acids and eicosanoid synthesis inhibitors on the growth of two human prostate cancer cell lines

Cited by 240 publications

References 24 publications

Multiplication and death-type of leukemia cell lines exposed to very long-chain polyunsaturated fatty acids

Multiplication and death-type of leukemia cell lines exposed to very long-chain polyunsaturated fatty acids

Cell cycle arrest and induction of apoptosis in pancreatic cancer cells exposed to eicosapentaenoic acid in vitro

Differential effect of polyunsaturated fatty acids on cell proliferation during human epithelial in vitro carcinogenesis: involvement of epidermal growth factor receptor tyrosine kinase

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