Effects of Fe (II)-chelating hairtail protein hydrolysates on the immune characteristics and intestinal microorganisms in loach (Misgurnus anguillicaudatus)

Lin, Haixin; Zeng, Caihong; Shui, Shanshan; Zhang, Bin

doi:10.1016/j.aqrep.2021.100630

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…The microbial community composition was then analyzed at the phylum, order, and family levels. For example, according to the phylum-level analysis, Proteobacteria presented the highest percentage, followed by Bacteroidetes and Firmicutes, regardless of the control and FLG-treated groups (Figure S12), consistent with the findings of previous studies that also identified Proteobacteria as the predominant bacterial phylum in the guts of loaches and other fishes. − However, FLG disturbed the composition of the microbiome at the phylum level by increasing the abundance of Bacteroidetes , Spirochaetes , Chloroflexi , and Cyanobacteria and decreasing the abundance of Proteobacteria (Figure S13A). Similar results were also observed at other taxonomic levels (Figure S13).…”

Section: Resultssupporting

confidence: 88%

Uptake Route Altered the Bioavailability of Graphene in Misgurnus anguillicaudatus: Comparing Waterborne and Sediment Exposures

Lü

Zha

Dong

et al. 2022

Environ. Sci. Technol.

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Numerous studies on the bioavailability of graphene-based nanomaterials relate to the water-only exposure route. However, the sediment exposure route should be the most important pathway for benthic organisms to ingest graphene, while to date little work on the bioavailability of graphene in benthic organisms has been explored. In this study, with the help of carbon-14-labeled few-layer graphene (14C-FLG), we quantificationally compared the bioaccumulation, biodistribution, and elimination kinetics of 14C-FLG in loaches via waterborne and sediment exposures. After 72 h of exposure, the accumulated 14C-FLG in loaches exposed via waterborne was 14.28 μg/g (dry mass), which was 3.18 times higher than that (4.49 μg/g) exposed via sediment. The biodistribution results showed that, compared to waterborne exposure, sediment exposure remarkably facilitated the transport of 14C-FLG from the gut into the liver, which made it difficult to be excreted. Although 14C-FLG did not cause significant hepatotoxicity, the disruption of intestinal microbiota homeostasis, immune response, and several key metabolic pathways in the gut were observed, which may be due to the majority of 14C-FLG being accumulated in the gut. Overall, this study reveals the different bioavailabilities of graphene in loaches via waterborne and sediment exposures, which is helpful in predicting its bioaccumulation capability and trophic transfer ability.

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Section: Resultssupporting

confidence: 88%

Uptake Route Altered the Bioavailability of Graphene in Misgurnus anguillicaudatus: Comparing Waterborne and Sediment Exposures

Lü

Zha

Dong

et al. 2022

Environ. Sci. Technol.

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“…Figure 6 shows the intervention mechanism of PSCP-Fe on IDA rats. In this study, we found that a low-Fe 2+ diet altered the structure of gut microbiota and increased the relative abundances of Lachnoclostridium , Adlercreutzia , Faecalibaculum, Coriobacteriaceae _UCG-002, and Subdoligranulum , which is consistent with the literature ( 53 ). Some beneficial bacteria were also recovered by administering PSCP-Fe.…”

Section: Discussionsupporting

confidence: 92%

A new iron supplement: The chelate of pig skin collagen peptide and Fe2+ can treat iron-deficiency anemia by modulating intestinal flora

et al. 2022

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IntroductionIron deficiency anemia (IDA) is one of the most common nutritional diseases encountered all over the world. Nowadays, oral iron supplementation is still the mainstay of IDA treatment.MethodsIn this study, a new iron nutritional supplement named pig skin collagen peptides ferrous chelates (PSCP-Fe) was prepared, and its structure was characterized by the scanning electron microscopy, sykam amino acid analyzer and Fourier transform infrared spectroscopy (FTIR). The anti-IDA activity of PSCP-Fe was evaluated in low-Fe2+ diet-induced IDA in rats. 16S amplicon sequencing technology was then used to reveal the mechanism of PSCP-Fe against IDA.ResultsThe results of amino acid analysis and FTIR showed that aspartic acid (Asp), arginine (Arg), histidine (His), glutamic acid (Glu), cystine (Cys), and lysine (Lys) residued in PSCP chelated readily with Fe2+ through their functional groups. PSCP-Fe treated reversed the hematology-related indexes, such as red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentrate (MCHC), serum ferritin (SF), serum hepcidin (HEPC) and serum transferrin receptor (TFR). And its regulatory action was better than that of FeSO4. Moreover, PSCP-Fe alleviated the hepatocyte apoptosis and necrosis, Fe2+ loss, and injury in IDA rats. In addition, PSCP-Fe could significantly retrace the disturbed profile of gut microbiota in IDA rats (p < 0.05) and significantly up-regulated the relative abundances of nine bacterial genus, including Lactobacillus, Alloprevotella, unclassified_of_Oscillospiraceae, and NK4A214_group (p < 0.05). It could also downgrade the relative abundances of Subdoligranulum and Coriobacteriaceae_UCG-002 (p < 0.05). The results of Spearman’s correlation analysis and distance-based redundancy analysis (db-RDA) revealed that Subdoligranulum and Christensenellaceae_R-7_group may be potential microbial markers for effective PSCP-Fe action in the treatment of IDA.DiscussionOverall, our results elucidate the interactions between gut bacteria and related cytokines and reveal the mechanisms underlying the anti-IDA effect of PSCP-Fe. They will thus provide a theoretical foundation for PSCP-Fe as a new iron nutritional supplement.

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“…The normal microflora can secrete various enzymes to promote the digestion and absorption of nutrients, and also regulate immune function to maintain body health ( Tran et al., 2018 ; Peng et al., 2019 ; Yang et al., 2021a ). Studies have shown that the main intestinal flora of loach include Proteobacteria and Actinobacteria ( Lin et al., 2021 ; Zhu et al., 2020 ; Yang et al., 2021a ); comparable findings were also obtained in this experiment. In our experiments, the relative abundances of Fusobacterium, Firmicutes and Bacteroidetes did not reach more than 10%, which may be due to different species, diets, sampling methods and analysis methods ( Nayak, 2010 ; Clements et al., 2014 ; Deng and Swanson, 2015 ).…”

Section: Discussionsupporting

confidence: 89%

Effects of different protein and lipid levels on the growth performance and intestinal microflora of loach (Paramisgurnus dabryanus)

Wang¹,

Li²,

Zhou³

et al. 2023

Animal Nutrition

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Effects of Fe (II)-chelating hairtail protein hydrolysates on the immune characteristics and intestinal microorganisms in loach (Misgurnus anguillicaudatus)

Cited by 4 publications

References 38 publications

Uptake Route Altered the Bioavailability of Graphene in Misgurnus anguillicaudatus: Comparing Waterborne and Sediment Exposures

Uptake Route Altered the Bioavailability of Graphene in Misgurnus anguillicaudatus: Comparing Waterborne and Sediment Exposures

A new iron supplement: The chelate of pig skin collagen peptide and Fe2+ can treat iron-deficiency anemia by modulating intestinal flora

Effects of different protein and lipid levels on the growth performance and intestinal microflora of loach (Paramisgurnus dabryanus)

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