Effects of feeding frequency on meat quality traits and <i>Longissimus muscle</i> proteome in finishing pigs

Liu, J. B.; Cai, Xianbin; Xiong, Hui; Zhang, H. F.

doi:10.1111/jpn.12636

Cited by 13 publications

(13 citation statements)

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“…Previous studies have shown that the biphasic feeding regimen improved the feed efficiency of pigs which were associated with altered energy partitioning and storage form (protein or lipid), as indicated by higher lean tissue content and/or decreased fat deposition in biphasic-fed pigs ( Newman et al, 2014 ; Liu et al, 2017 ; Yan et al, 2020 ). Gut microbiota have been recognized as a critical factor influencing the energy partitioning between lean and fat tissues of the host ( Bäckhed et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, feeding frequency has been regarded as a critical factor that affects weight and carcass composition in pigs ( Le Naou et al, 2014 ). Some studies have demonstrated that reducing meal frequency impaired the feed efficiency, while other studies contend that reduced meal frequency elevated the feed efficiency of pigs ( Schneider et al, 2011 ; Colpoys et al, 2016 ; Liu et al, 2017 ). A previous study has shown that the duration of each feeding bout is critical for the improvement effect of reduced meal frequency on feed conversion efficiency ( Newman et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…A previous study has shown that the duration of each feeding bout is critical for the improvement effect of reduced meal frequency on feed conversion efficiency ( Newman et al, 2014 ). After the comparison of the feeding regimen design between previous studies, it can be summarized that pigs fed for two 60-min intervals exhibited improved feed efficiency compared to those fed for a higher meal frequency or ad libitum ( Le Naou et al, 2014 ; Newman et al, 2014 ; Liu et al, 2017 ). Under the condition of reduced feeding frequency, the improved feed efficiency might be attributed to the changes in energy partitioning or the form of energy storage ( Van Milgen, 2002 ; Le Naou et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Reduced Feeding Frequency Improves Feed Efficiency Associated With Altered Fecal Microbiota and Bile Acid Composition in Pigs

Yan

Wei

et al. 2021

Front. Microbiol.

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A biphasic feeding regimen exerts an improvement effect on feed efficiency of pigs. While gut microbiome and metabolome are known to affect the host phenotype, so far the effects of reduced feeding frequency on fecal microbiota and their metabolism in pigs remain unclear. Here, the combination of 16S rRNA sequencing technique as well as untargeted and targeted metabolome analyses was adopted to investigate the fecal microbiome and metabolome of growing–finishing pigs in response to a biphasic feeding [two meals per day (M2)] pattern. Sixty crossbred barrows were randomly assigned into two groups with 10 replicates (three pigs/pen), namely, the free-access feeding group (FA) and the M2 group. Pigs in the FA group were fed free access while those in the M2 group were fed ad libitum twice daily for 1 h at 8:00 and 18:00. Results showed that pigs fed biphasically exhibited increased feed efficiency compared to FA pigs. The Shannon and Simpson indexes were significantly increased by reducing the feeding frequency. In the biphasic-fed pigs, the relative abundances of Subdoligranulum, Roseburia, Mitsuokella, and Terrisporobacter were significantly increased while the relative abundances of unidentified_Spirochaetaceae, Methanobrevibacter, unidentified_Bacteroidales, Alloprevotella, Parabacteroides, and Bacteroides were significantly decreased compared to FA pigs. Partial least-square discriminant analysis (PLS-DA) analysis revealed an obvious variation between the FA and M2 groups; the differential features were mainly involved in arginine, proline, glycine, serine, threonine, and tryptophan metabolism as well as primary bile acid (BA) biosynthesis. In addition, the changes in the microbial genera were correlated with the differential fecal metabolites. A biphasic feeding regimen significantly increased the abundances of primary BAs and secondary BAs in feces of pigs, and the differentially enriched BAs were positively correlated with some specific genera. Taken together, these results suggest that the improvement effect of a reduced feeding frequency on feed efficiency of pigs might be associated with the altered fecal microbial composition and fecal metabolite profile in particular the enlarged stool BA pool.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reduced Feeding Frequency Improves Feed Efficiency Associated With Altered Fecal Microbiota and Bile Acid Composition in Pigs

Yan

Wei

et al. 2021

Front. Microbiol.

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“…A twelve meals regimen increased the liver concentration of total lipid, glycogen, and triglyceride than that in the two meals daily pigs [8]. Compared with pigs fed ad libitum, the activities of citrate synthase, β-hydroxylacyl-CoA dehydrogenase, and lactate dehydrogenase were greater in the longissimus muscle of the two meals daily regimen [9]. The intestine and liver are the major digestive, absorption, and metabolism organs of the body and play a key role in the metabolism of nutrients [10,11].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Responses in the Livers and Jejunal Mucosa of Pigs under Different Feeding Frequencies

Zhang

Liu

Zhang

et al. 2019

Animals

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Simple SummaryNutrition management strategies are closely related to body development and health, and feeding frequency affects pig feed intake, feed efficiency, body composition, and growth performance. However, the effect of feeding one time daily and two times daily on the intestine has been given less attention. In this study, we investigated the transcriptomic responses induced in the livers and jejunal mucosa of growing pigs by daily feeding schedules. We found that when compared with feeding once daily, two times feeding had no significant effect on the growth performance of growing pigs with the same average daily feed intake. A two meals regimen reduced the concentration of triglycerides in serum and liver, affected the body metabolism by promoting lipid transport, lipogenesis, fatty acid oxidation, chylomicron formation and transport, gluconeogenesis, and inhibiting adipocyte differentiation. These findings support the idea that different feeding regimens could affect lipid metabolism and can be effective in nutritional strategies against metabolic dysfunction.AbstractFeeding frequency in one day is thought to be associated with nutrient metabolism and the physical development of the body in both experimental animals and humans. The present study was conducted to investigate transcriptomic responses in the liver and jejunal mucosa of pigs to evaluate the effects of different feeding frequencies on the body’s metabolism. Twelve Duroc × Landrance × Yorkshire growing pigs with an average initial weight (IW) of 14.86 ± 0.20 kg were randomly assigned to two groups: feeding one time per day (M1) and feeding two times per day (M2); each group consisted of six replicates (pens), with one pig per pen. During the one-month experimental period, pigs in the M1 group were fed on an ad libitum basis at 8:00 am; and the M2 group was fed half of the standard feeding requirement at 8:00 am and adequate feed at 16:00 pm. The results showed that average daily feed intake, average daily gain, feed:gain, and the organ indices were not significantly different between the two groups (p > 0.05). The total cholesterol (T-CHO), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C) concentrations in the serum, and the TG concentration in the liver in the M2 groups were significant lower than those in the M1 group, while the T-CHO concentration in the liver were significant higher in the M2 group (p < 0.05). Jejunal mucosa transcriptomic analysis showed the gene of Niemann-Pick C1-Like 1 (NPC1L1), Solute carrier family 27 member 4 (SLC27A4), Retinol binding protein 2 (RBP2), Lecithin retinol acyltransferase (LRAT), Apolipoprotein A (APOA 1, APOA 4, APOB, and APOC 3) were upregulated in the M2 group, indicating that fat digestion was enhanced in the small intestine, whereas Perilipin (PLIN1 and PLIN2) were downregulated, indicating that body fat was not deposited. Fatty acid binding proteins (FABPs) and Acetyl-CoA acyltransferase 1 (ACAA1) were upregulated in the M2 group, i...

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“…The results presented above indicated that the level of ALD could be another significant factor in muscle glycolysis after slaughter beside other enzymes (e.g., phosphofructokinase, glycogen phosphorylase) (Apaoblaza et al, ; England et al, ). Moreover, Liu, Cai, Xiong, and Zhang () demonstrated a significant influence of feeding frequency regimes of pigs (free access to diet and feeding twice a day) on proteome of Longissimus muscle. The results showed that energy expenditure was increased by reducing meal frequency.…”

Section: Discussionmentioning

confidence: 99%

The effect of inulin supply to high‐fat diet rich in saturated fatty acids on pork quality and profile of sarcoplasmic protein in meat exudate

Przybylski

Jaworska

Sałek

et al. 2018

Animal Physiology Nutrition

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The aim of the study was to evaluate the influence of inulin supply to high‐fat diet rich in saturated fatty acids (SFA) on pork quality and profile of sarcoplasmic protein in drip loss. At 50 days of age, twenty cross‐bred pigs (gilts) were randomly allotted to four groups: the control (C) group fed a standard diet, and three experimental (D1, D2 and D3) groups fed a high‐fat diet rich in SFA. Moreover, pigs from the groups D2 and D3 consumed an extra inulin supply (7% of daily feed intake) from 85 to 120 days of age (for 5 weeks) and from 50 to 120 days of age (for 10 weeks) respectively. The addition of inulin to the diet reduced meaty odour and flavour significantly, improved tenderness and overall sensory quality of pork and additionally influenced ultimate pH, L* colour parameter, lactate level and protein content in meat. The diets also affected the profile of sarcoplasmic proteins. Significant effects were observed for the following enzymes—PK/PGI (pyruvate kinase/phosphoglucose isomerase) and ALD (aldolase), which are related to the intensity of post‐mortem glycolysis. Presented data indicate that long‐term inulin supply to high‐fat diet has a positive effect on technological and sensory quality as well as protein profile of pork.

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Effects of feeding frequency on meat quality traits and Longissimus muscle proteome in finishing pigs

Cited by 13 publications

References 45 publications

Reduced Feeding Frequency Improves Feed Efficiency Associated With Altered Fecal Microbiota and Bile Acid Composition in Pigs

Reduced Feeding Frequency Improves Feed Efficiency Associated With Altered Fecal Microbiota and Bile Acid Composition in Pigs

Transcriptomic Responses in the Livers and Jejunal Mucosa of Pigs under Different Feeding Frequencies

The effect of inulin supply to high‐fat diet rich in saturated fatty acids on pork quality and profile of sarcoplasmic protein in meat exudate

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