Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture

Abstract

Rotifer growth performance and microbial community changes associated with rotifer cultures were monitored while different feed types (Nannochloropsis oculata paste and the commercial yeast based feed CS-3000), different regimes (daily changes, changes per batch and no changes) and mixtures of three probionts (Phenylobacterium sp.; Gluconobacter sp. and Paracoccus denitrificans) were provided. It was shown that the dominant bacterial species in the cultures receiving either N. oculata or CS-3000 were different. However, in cultures receiving both feeds (either switching between feeds on a daily basis or on a batch basis), a high similarity in microbial community fingerprint was found. The presence of probionts was detected by the end of four batch culture cycles in spite of strong shifts of the bacterial community. By group discriminant analysis, it was found that Phenylobacterium sp. and Paracoccus sp. contributed positively to the CS-3000-fed group, while Gluconobacter sp. contributed positively to the N. oculata-fed group, although they did not appear as very dominant species

Microbial manipulations to improve fish health and production – A Mediterranean perspective

“…Gatesoupe [169] Assess the effects of LAB on the production of rotifers LAB strains L. plantarum decreased the proportion of A. salmonicida-like bacteria associated with rotifers. Qi et al [170] Assess effect of probionts on rotifer microbial composition and growth performance Phenylobacterium spp., Gluconobacter spp. and Paracoccus denitrificans…”

Probiotic Applications in Crustaceans

“…Artemia like other live feed can be a source of pathogenic bacteria in hatchery environments (Avila-Villa et al 2011;Chapter 16) and probiotics have been proposed as a solution to reduce the risks of contamination (Meunpol et al 2003;Vershuere et al 2000a). Changes in bacterial assemblages as well as protection against pathogens have often been measured when probiotics are applied to live feeds including Artemia and rotifers (Verschuere et al 1999;Marques et al 2006a;Rojas-Garcia et al 2008;Qi et al 2008;Pintado et al 2010).…”

Technical and economic feasibility of adding pure oxygen for rotifer production (Brachionus plicatilis) spotted snapper (Lutjanus guttatus) larvae feeding

“…The reported cost for a million of rotifers in this study was higher than those published by Bentley et al, (2008)($ 0.009) and Suantika et al, (2003) ($ 0.048), however, it was lower than Alvarez- Lajonchere and Álvarez et.al, (2013) at a cost of $ 1.65 in batch cultures. Oxygen is used by many authors as a resource to support the stability of rotifer cultures (Kostopoulou et al, 2006;Sayegh et al, 2007;Papakostas et al, 2007;Kobayashi et al ., 2008;Larsen et al 2008;Yin and Zhao, 2008;Cavalin and Weirich, 2009;Mahmoudzadeh et a.l, 2009;Qi et al, 2009), however, in the Pacific Marine Park adding oxygen posed no technical difference (ANOVA p> 0.05) compared to control culture due to several factors; the first is related to the fact that dissolved oxygen was controlled only during the day (it was not mechanized and there were workers who labored night shift) which did not allow a good control of this variable. According to Alvarez-Lajonchere and Álvarez et.al, (2013) results in rotifer cultures depend on the correct technology developed in the laboratory.…”

“…This has encouraged to research on the conditions that optimize the growth of populations of this organism (Kostopoulou et al, 2006;Sayegh et al, 2007;Papakostas et al, 2007;Kobayashi et al, 2008;Larsen et al, 2008;Yin and Zhao, 2008;Cavalin and Weirich, 2009;Mahmoudzadeh et al, 2009;Qi et al, 2009), specifically, may studies related to the addition of pure oxygen as support for rotifer cultures (Yoshimura et al, 1996;Dhert et al, 2001;Yoshimura et al, 2003), however, is necessary to evaluate the economic conditions as well as staff availability and infrastructure before deciding the technique with which to work, as noted by Suantika et al (2003) in their study on rotifers production progress.…”