Effects of fermented herbal tea residue on meat quality, rumen fermentation parameters and microbes of black goats

Wang, Mingyue; Wu, Longfei; Guo, Yongqing; Sun, Jiajie; Deng, Ming; Liu, Guangbin; Li, Yaokun; Sun, Baoli

doi:10.1186/s13568-023-01610-2

Cited by 5 publications

(1 citation statement)

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“…Firmicutes and Bacteroidetes are the dominant phyla in the rumen microbial community and play a key role in the degradation of roughage [ 37 , 38 , 39 ], which is consistent with our research findings. Studies have shown that Firmicutes contain many bacteria involved in the efficient degradation and utilization of cellulose [ 40 , 41 ]. In this experiment, the relative abundance of Firmicutes in AS at 72 h was significantly higher than that at 12 h, which may be related to the higher cellulose content in AS.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Gu,

Liu,

Jiang

et al. 2024

Animals

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The purpose of this study was to study the chemical composition, rumen degradation characteristics, surface attached microbial community and cellulase activity of garlic skin (GS) and Artemisia argyi stalk (AS), in order to explain their feeding value. Four 14-month-old healthy Min Dong male goats with permanent rumen fistula were selected as experimental animals. The rumen degradation characteristics of GS and AS were determined by using the nylon bag method, and the bacterial composition, cellulase activity and their relationship on the surface of the two groups were analyzed with high-throughput sequencing of 16S rRNA gene. The results showed that in GS and AS, the effective degradation rate (ED) values of dry matter (DM) were 42.53% and 37.12%, the ED values of crude protein (CP) were 37.19% and 43.38%, the ED values of neutral detergent fiber (NDF) were 36.83% and 36.23%, and the ED values of acid detergent fiber (ADF) were 33.81% and 34.77%. During rumen degradation, the richness and evenness of bacteria attached to the AS surface were higher. At the phylum level, Bacteroidetes and Firmicutes were always the main rumen bacteria in the two groups. At the genus level, fiber-degrading bacteria such as Prevotella, Treponema, and Ruminococcus showed higher levels in GS (p < 0.05). Compared with GS, the activity of β-glucosidase (BG enzyme), endo-β-1,4-glucanase (C1 enzyme), exo-β-1,4-glucanase (Cx enzyme) and neutral xylanase (NEX enzyme) attached to AS surface showed a higher trend. Correlation analysis showed that the relative abundance of Succinivibrio and Rikenellaceae_RC9_gut_group was positively correlated with the rumen degradability of nutrients in GS, and the relative abundance of Christensenellaceae R-7_group, Succinivibrio and Ruminococcus was positively correlated with the rumen degradability of nutrients in AS. The conclusion of this study shows that AS has more potential to become ruminant roughage than GS. In addition, this study also revealed the relationship between cellulase activity and bacteria, which provided new information for us to better analyze the effects of GS and AS on the rumen of ruminants and provided an important theoretical basis for the development and utilization of agricultural by-products.

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Section: Discussionmentioning

confidence: 99%

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Gu,

Liu,

Jiang

et al. 2024

Animals

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Ensiled diet improved the growth performance of Tibetan sheep by regulating the rumen microbial community and rumen epithelial morphology

Zhao,

Gao

et al. 2024

Journal of Animal Science

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The aim of this study was to investigate the effects of ensiled agricultural byproducts from Qinghai-Tibet plateau on growth performance, rumen microbiota, ruminal epithelium morphology and nutrient transport-related gene expression in Tibetan sheep. Fourteen male Tibetan sheep were randomly assigned to one of two diets: an untreated diet (without silage inoculum, CON, n=7) or an ensiled diet (with silage inoculum, ESD, n=7). The total experimental period lasted for 84 days, including early 14 days as adaption period and remaining 70 days for data collection. The ESD increased average daily gain (ADG, P = 0.046), dry matter intake (P < 0.001), ammonia nitrogen (NH3-N, P = 0.045), microbial crude protein (MCP, P = 0.034) and total volatile fatty acids (VFA) concentration (P < 0.001), and decreased ruminal pH value (P = 0.014). The proportion of propionate (P = 0.046) and the copy numbers of bacteria (P = 0.01) and protozoa (P = 0.002) were higher, while the proportion of acetate (P = 0.028) was lower in the sheep fed ESD compared to CON. Pyrosequencing of the 16S ribosomal RNA gene revealed that ESD increased the relative abundance of Firmicutes, Ruminococcus, Lachnospiraceae_AC2044_group, Lachnospiraceae_XPB1014_group, Christensenellaceae_R-7_group in the rumen (P < 0.05), while decreased the relative abundance of Bacteroidota, Prevotellaceae_UCG-003, and Veillonellaceae_UCG-001 (P < 0.05). Analyses with PICRUSt2 and STAMP indicated that the propionate metabolism pathway was enriched in the sheep fed ESD (P = 0.026). The ESD increased the rumen papillae height (P = 0.012), density (P = 0.036), and surface area (P = 0.001), and improved the thickness of the total epithelia (P = 0.018), stratum corneum (P = 0.040), stratum germinativum (P = 0.042), and stratum spinosum and basale (P = 0.004). The relative mRNA expression of Cyclin-dependent Kinase 2 (CDK2), CyclinA2, CyclinD2, zonula occludens (ZO-1), Occludin, monocarboxylate transporter isoform 1 (MCT1), monocarboxylate transporter isoform 4 (MCT4), sodium/potassium pump (Na+/K+-ATPase), and sodium/hydrogen antiporter 3 (NHE3) were higher in the rumen epithelial of sheep fed ESD than CON (P < 0.05). Conversely, the relative mRNA expressions of Caspase 3 and B-cell lymphoma-2 (Bcl-2) were lower in the sheep fed ESD than CON (P < 0.05). In conclusion, compared with untreated diet, feeding an ensiled diet altered the rumen microbial community, enhanced nutrient transport through rumen epithelium, and improved the growth performance of Tibetan sheep.

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High-concentrate diet supplemented with hydrolysable tannin improves the slaughter performance, intestinal antioxidant ability and barrier function of fattening lambs

Ma,

Li,

Lin

et al. 2024

Front. Vet. Sci.

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The objective of current experiment was to study the potential influence of hydrolysable tannin supplementation on slaughter performance, meat quality, intestinal digestive enzyme activity, antioxidant ability and barrier function in fattening lambs. In total, 36 male Hu sheep lambs with similar body weight (15.83 ± 0.48 kg) and days in age (55 ± 2 d) were randomly assigned to one of three groups of 12 animals each: control without tannin (CON) and tannin supplementation groups (TA1, 3 g/d per lamb; TA2, 6 g/d per lamb). All the lambs were reared in individual hutches, and the experiment lasted for 60 d. On d 61, 8 lambs from each group were randomly selected to slaughter. Results showed that the serum diamine oxidase and lipopolysaccharide contents of TA2 group were higher (p < 0.05) than those of CON group. Compared with CON group, the carcass weight and intramuscular fat content of lambs in TA1 group were increased (p < 0.05) and the meat shear force was decreased (p < 0.05). The trypsin activity in the jejunum and ileum of TA1 group was higher (p < 0.05) than that of CON and TA2 groups. Also, tannin supplementation significantly increased (p < 0.05) the level of jejunal and ileal total antioxidant capacity and reduced (p < 0.05) the jejunal malondialdehyde concentration in lambs. The jejunum and ileum of TA1 lambs showed reduced (p < 0.05) tumor necrosis factor-alpha and increased (p < 0.05) interleukin-10 mRNA levels compared with CON lambs. In the jejunum, the secretory immunoglobulin A content of TA1 group was higher (p < 0.05) than that of CON and TA2 groups. Lambs supplemented with tannin at the level of 3 g/d increased (p < 0.05) the gene expressions of claudin-1, claudin-4 and zonula occludens-1 in the jejunum when compared to those of CON and TA2 groups. In summary, tannin supplementation at the level of 3 g/d per animal can improve the production performance and intestinal function of fattening lambs fed a high-concentrate diet.

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Effects of fermented herbal tea residue on meat quality, rumen fermentation parameters and microbes of black goats

Cited by 5 publications

References 50 publications

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Ensiled diet improved the growth performance of Tibetan sheep by regulating the rumen microbial community and rumen epithelial morphology

High-concentrate diet supplemented with hydrolysable tannin improves the slaughter performance, intestinal antioxidant ability and barrier function of fattening lambs

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