Effects of Fisetin on Allergic Contact Dermatitis via Regulating the Balance of Th17/Treg in Mice

Abstract

Background. Allergic contact dermatitis (ACD) is a form of chronic cutaneous inflammatory disease of immunological origin that has adverse impacts on patient quality of life, underscoring the need for the development of safe and effective therapeutic agents to treat affected individuals. Fisetin is a Chinese herbal preparation that reportedly exhibits antitumor, antioxidant, antimicrobial, anticoagulatory, and antimalarial activity. In the current report, the immunomodulatory activity of fisetin was appraised by assessing its impact on balance between regulatory T (Treg) and Th17 cells in an ACD model. Methods. BALB/c mice ( n = 60 ) were randomized into control, ACD model, CTX positive control (20 mg/kg), and fisetin treatment groups (three dose levels: 2, 4, or 8 mg/kg). ACD induction was achieved by sensitizing mice on the shaved ventral abdomen via the application of 5% DNFB (50 μL) on days 1 and 2, followed by rechallenge in the right ear with 5% DNFB (20 μL) on day 5. Beginning on day 1, immunized mice were intraperitoneally injected with the appropriate fisetin dose (in saline) once per day for 7 days. On day 7, ear swelling, transcription factor expression, Th17/Treg cell populations, and cytokine production were assessed in vivo. Results. Fisetin treatment significantly suppressed ear swelling and associated inflammatory cell infiltration, besides reducing the production of Th17 cytokines (IL-17, TNF-α, and IL-6) and the expression of the Th17 lineage transcription factor RORγt while simultaneously enhancing Treg-specific cytokine production (TGF-β and IL-10) and the expression of the Treg lineage transcription factor Foxp3, thereby restoring the Th17/Treg cell in ACD mice. Conclusions. These data indicate that fisetin exhibits immunomodulatory activity and can alter the Th17/Treg cell balance, highlighting its potential value as a treatment drug for ACD.

Evaluation of the anti-inflammatory activity of fisetin-loaded nanoparticles in an in vitro model of osteoarthritis

“…8 F). The results indicated that although the IL-10 level was decreased in chondrocytes stimulated with IL-1β compared with the untreated group (control group), the treatment with FNPs, like fisetin 48 , 49 , increased its level in IL-1β pretreated human chondrocytes. In general, these results showed that FNPs can inhibit the inflammatory responses induced by IL-1β, including the expression of TNF-α, and IL-6, and reduce the degradation of Sox-9, COL2, and SirT1 in OA chondrocyte cells.…”

“…However, similar to fisetin, FNPs reduced TNF-α and IL-6 mRNA expression in IL-1β stimulated cells. Next, the effect of FNPs on the mRNA expression of IL-10, a well-known anti-inflammatory cytokine 48 , 49 , was investigated in IL-1β stimulated human chondrocytes (Fig. 8 F).…”

Retracted: Effects of Fisetin on Allergic Contact Dermatitis via Regulating the Balance of Th17/Treg in Mice

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”