Effects of flaxseed dietary supplementation on sperm quality and on lipid composition of sperm subfractions and prostatic granules in rabbit

Mourvaki, Evangelia; Cardinali, R.; Bosco, Alessandro Dal; Corazzi, Lanfranco; Castellini, Cesare

doi:10.1016/j.theriogenology.2009.10.019

Cited by 76 publications

(74 citation statements)

References 28 publications

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“…The absence of positive effects on the freezability of sperm in this study was unexpected because supplementation with omega-3 increases the antioxidant activity of seminal fluid, which prevents the oxidation of macromolecules (DNA, proteins and lipids) (MAZZA et al, 2007), allows the greater protection of sperm membranes via the inhibited lipid peroxidation, and ultimately contributes to a higher quality of semen and, in particular, increased motility after thawing (AURICH et al, 1997;MICHAEL et al, 2009;MOURVAKI et al, 2010). However, Esmaeili et al (2012) observed superior sperm parameters in ovine semen after thawing.…”

Section: Discussionmentioning

confidence: 79%

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/ thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1 st -5 th week) or pre-supplementation; M2 (6 th -9 th week) and M3 (10 th -13 th week) or during supplementation; and M4 (14 th -17 th week) or postsupplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen. Key words: Canine. Diet-supplemented. Polyunsaturated-fatty-acid. Sperm. ResumoOs ácidos graxos têm sido recomendados há anos como suplemento dietético para a melhora do pelo canino. Na área de reprodução animal, a suplementação com ômega tem sido usada para aumentar a eficiência reprodutiva e a taxa de concepção em muitas espécies, mas poucos estudos foram conduzidos em cães. Então, o objetivo deste estudo foi avaliar os efeitos da suplementação dietética diária com ômega-3 e -6 sobre a qualidade do sêmen fresco e congelado de cães. de 7 cães e avaliados quanto a motilidade espermática, vigor, concentração e morfologia. O estudo foi dividido de acordo com os períodos de suplementação oral com omegas-3 e -6, no qual M1 (1ª-5ª semana) foi considerado o período pré-suplementação, M2 (6ª-9ª semana) e M3 (10ª-13ª semana) período de suplementação e M4 (14ª-17ª semana) pós-suplementação, totalizando 17 semanas de estudo (119 ejaculados). Após a análise, o sêmen foi congelado e reavaliado imediatamente e 30 minutos após a descongelação (mantidas a 37º C). A suplementação resultou em aumento da motilidade espermática, vigor e concentração do sêmen fresco; no entanto, não houve influência na congelabilidade do sêmen. Além disso, não houve aumento da motilidade dos espermatozoides após a suplementação, quando as células descongeladas foram mantidas a 37° C, durante 30 minutos. Concluiu-se que a suplementação dietética com omegas-3 e -6, durante 4 a 8 semanas melhora os parâmetros do sêmen fresco de cães, embora não há nenhum efeito sobre a qualidade espermática após a congelação. Palavras-chave: Ácido-graxo-poli-insaturado. Canino. ...

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Section: Discussionmentioning

confidence: 79%

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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“…In the literature, a high content of PUFA in the chicken sperm membrane influences sperm function and modification of dietary fatty acids can affect spermatozoa (Bongalhardo et al, 2009). In addition, Mourvaki et al (2010) concluded that daily supplementation with n-3, n-6 and n-9 fatty acids significantly increased semen volume and concentration, while decreasing abnormal spermatozoa. SL improved semen quality, biochemical constituents of blood and seminal plasma and the antioxidant status of both Means within a row with different superscripts are significantly different (P , 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Lipids are involved in the structural and functional functions of the sperm, and their profile may be modified according to physiologic events and/or diet (Mourvaki et al, 2010). Animals cannot synthesize polyunsaturated fatty acids (PUFA) n-6 or n-3 fatty acids de novo as they lack the appropriate fatty acid desaturase enzymes.…”

Section: Introductionmentioning

confidence: 99%

Semen quality, testosterone, seminal plasma biochemical and antioxidant profiles of rabbit bucks fed diets supplemented with different concentrations of soybean lecithin

Attia

Kamel

2012

Animal

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A total of 28 adult V-line rabbits were fedad libituma control diet or a diet supplemented with 0.5%, 1.0% and 1.5% soybean lecithin (SL) for 12 weeks. Bucks that received 0.5%, 1.0% or 1.5% dietary SL had a higher ejaculate volume, mass motility, sperm concentration, total sperm output and total motile sperm. Dietary SL reduced the percentage of dead sperm and increased the normal sperm, and this concurred with an increase in blood testosterone concentration. Blood and seminal plasma total lipid, acid phosphatase and seminal plasma alkaline phosphatase were significantly increased because of inclusion of SL. Interestingly, SL reduced blood and seminal plasma thiobarbituric acid-reactive substances while increasing blood and seminal plasma glutathione content, glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity. Conception rate and litter size at birth and weaning were also significantly improved. Practically, it could be suggested that SL is a suitable supplement for improving semen quality, antioxidant status, reproductive traits and the economic efficiency of V-line rabbit bucks and 1% is an adequate concentration.

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“…The presence of unsaturated fatty acids increases the susceptibility of spermatozoa to peroxidation, which contributes to a negative effect on semen quality (de Lamirande et al, 1997;Bansal and Bilaspuri, 2011). According to some authors (Mournaki et al, 2010;Castellini et al, 1999Castellini et al, , 2003Castellini, 2008), the administration of antioxidants such as vitamin E, selenium, vitamin C, and carotenoids may reduce the oxidative stress and improve sperm motility (Agarwal et al, 2004). In particular, the role of antioxidants is to contrast the spermatic cell membrane lipid peroxidation and sperm DNA fragmentation caused by reactive oxygen species, responsible for male infertility in animals and man (Baker et al, 1996;Comhaire et al, 2000;Aitken et al, 2003;Greco et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of lycopene supplementation on semen quality and reproductive performance in rabbit

et al. 2012

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A total of 18 hybrid strain Martini male rabbits were randomly divided into 3 experimental groups. The animals in the first and second groups drank water with 0.1 and 0.5 g/L of lycopene addition (0.1 and 0.5 groups, respectively), while water without any supplement was administered to the control group rabbits (0 group) for 8 wk. Semen was collected from 18 bucks (6 animals/group) for 5 consecutive weeks. Ejaculate volume was determined by graduated test tube and sperm concentration was calculated by Neubauer chamber. Sperm motility was evaluated subjectively by a phase contrast microscope and forward progressive motility (FPM) scored 1-4 (low-high). Sperm viability was assessed by nigrosin/eosin (N/E) staining procedure. At the end of the semen evaluation period, 20 females/group were inseminated on day 11 post partum with a pool of semen collected from each experimental group. Data showed that the highest level of lycopene (0.5 group) resulted in a significantly greater volume of ejaculate and total number of sperm than in the control group (0.98 vs. 0.78 mL and 364 vs. 227; P<0.05), while sperm concentration was not affected. Lycopene addition did not significantly affect the sperm quality of fresh semen, but influenced the semen traits during storage (24 h at 5°C). In particular, the motility, FPM and viability of the 0.5 group were significantly higher (P<0.05) than those found in the 0 and 0.1 groups. No significant differences were found in reproductive performance. This study shows the positive effects of lycopene supplementation on semen production and refrigerated semen characteristics. Lycopene supplementation of drinking water could have interesting applications in field conditions, although further research into the role of this antioxidant in animal production is needed.

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Effects of flaxseed dietary supplementation on sperm quality and on lipid composition of sperm subfractions and prostatic granules in rabbit

Cited by 76 publications

References 28 publications

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Semen quality, testosterone, seminal plasma biochemical and antioxidant profiles of rabbit bucks fed diets supplemented with different concentrations of soybean lecithin

Effect of lycopene supplementation on semen quality and reproductive performance in rabbit

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