Effects of fluctuating temperature and silicate supply on the growth, biochemical composition and lipid accumulation of Nitzschia sp.

Jiang, Yuelu; Laverty, Katherine Starks; Brown, Jola; Nunez, Marcella; Brown, Lou; Chagoya, Jennifer; Burow, Mark D.; Quigg, Antonietta

doi:10.1016/j.biortech.2013.12.068

Cited by 20 publications

(5 citation statements)

References 35 publications

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“…(strain ArM0029B) displayed fast growth rates over a broad temperature range of 4-32°C (Ahn et al 2012;Jeong et al 2014). Long-term exposure to the extremely low temperatures may induce the development of adaptive mechanisms to chilling in photosynthesis (Hawes 1990;Anning et al 2001), biochemical composition (Converti et al 2009;Sang et al 2012;Jiang et al 2014), and even in gene expression (Chong et al 2011;An et al 2013). For example, elevated nitrate reductase activity and photosynthetic rates enhance the chance of survival of Stichococcus in Antarctica (Chen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The eurythermal adaptivity and temperature tolerance of a newly isolated psychrotolerant Arctic Chlorella sp.

Cao

Yang

et al. 2015

J Appl Phycol

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A new strain of Chlorella sp. (Chlorella-Arc), isolated from Arctic glacier melt water, was found to have high specific growth rates (μ) between 3 and 27°C, with a maximum specific growth rate of 0.85 day −1 at 15°C, indicating that this strain was a eurythermal strain with a broad temperature tolerance range. To understand its acclimation strategies to low and high temperatures, the physiological and biochemical responses of the Chlorella-Arc to temperature were studied and compared with those of a temperate Chlorella pyrenoidosa strain (Chlorella-Temp). As indicated by declining F v /F m , photoinhibition occurred in Chlorella-Arc at low temperature. However, Chlorella-Arc reduced the size of the light-harvesting complex (LHC) to alleviate photoinhibition, as indicated by an increasing Chl a/b ratio with decreasing temperatures. Interestingly, Chlorella-Arc tended to secrete soluble sugar into the culture medium with increasing temperature, while its intracellular soluble sugar content did not vary with temperature changes, indicating that the algal cells might suffer from osmotic stress at high temperature, which could be adjusted by excretion of soluble sugar. Chlorella-Arc accumulated protein and lipids under lower temperatures (<15°C), and its metabolism switched to synthesis of soluble sugar as temperatures rose. This reflects a flexible ability of Chlorella-Arc to regulate carbon and energy distribution when exposed to wide temperature shifts. More saturated fatty acids (SFA) in Chlorella-Arc than Chlorella-Temp also might serve as the energy source for growth in the cold and contribute to its cold tolerance.

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Section: Introductionmentioning

confidence: 99%

The eurythermal adaptivity and temperature tolerance of a newly isolated psychrotolerant Arctic Chlorella sp.

Cao

Yang

et al. 2015

J Appl Phycol

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“…HB162 in our study, its specific growth rate is much lower. Jiang et al [38] revealed that the optimal season for Nitzschia sp. to produce biomass was summer (23-37°C) but the best lipid production occurred in winter (2-17°C).…”

Section: Temperature Adaptability Of 6 Representative Diatom Strainsmentioning

confidence: 99%

Screening of High Temperature-Tolerant Oleaginous Diatoms

Zhang¹,

Hu²,

Wan³

et al. 2020

J. Microbiol. Biotechnol.

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Screening suitable strains with high temperature adaptability is of great importance for reducing the cost of temperature control in microalgae cultivation, especially in summer. To obtain high temperature-tolerant diatoms, water samples were collected in summer from 7 different regions of China across the Northeast, North and East. A total of 731 water samples was collected and from them 131 diatom strains were isolated and identified based on the 18S rRNA sequences. Forty-nine strains out of the 131 diatoms could survive at 30°C, and 6 strains with relatively high biomass and lipid content at high temperature were selected and were found to be able to grow at 35°C. Cyclotella sp. HB162 had the highest dry biomass of 0.46 g/l and relatively high triacylglycerol (TAG) content of 237.4 mg/g dry biomass. The highest TAG content of 246.4 mg/g dry biomass was obtained in Fistulifera sp. HB236, while Nitzschia palea HB170 had high dry biomass (0.33 g/l) but relatively low TAG content (105.9 mg/g dry biomass). N. palea HB170 and Fistulifera sp. HB236 presented relatively stable growth rates and lipid yields under fluctuating temperatures ranging from 28 to 35°C, while Cyclotella HB162 maintained high lipid yield at temperatures below 25°C. The percentage of saturated fatty acids and monounsaturated fatty acids in all the 6 strains was 84-91% in total lipids and 90-94% in TAGs, which makes them the ideal feedstock for biodiesel.

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“…This result was expected because most of the biochemical compounds, except for protein, increased when the culture aged, especially carbohydrate and lipid (Fidalgo et al, 1998;Lin et al, 2007;Chiu et al, 2009). Furthermore, phytoplankton may physiologically acclimate in response to variation in temperature, changing their biochemical composition or adjusting their membrane lipid to increase their capacity to grow or survive (Jiang et al, 2014). Besides that, data presented in the literature show that the lipid content in marine algae are less than 4% of the dry weight depending on the species (Herbreteau et al, 1997;McDermind and Stuercke, 2003).…”

Section: Lipid Determinationmentioning

confidence: 99%

Proximate and biochemical analysis for marine and freshwater algae

Puspanadan¹,

Maznah²,

LeeChee³

2018

MJM

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In this study, ten indigenous microalgae samples from freshwater and marine waters from Malaysia, cultured and analysed on proximate and biochemical analysis. The proximate and biochemical analysis consists of starch, carbohydrates, lipid, protein, ash and moisture contents. This study was more focused on screening of starch accumulation in marine and freshwater microalgae cultures. Methodology and results: Based on screening, the results showed that Chlorella salina contents highest starch of 4.92±0.33%, followed by Spirulina sp. 2.58±1.18%, Isochrysis maritime 0.99±0.33%, and lastly for Nitzschiapanduriformis and Naviculadistans contents similar percentage of starch (0.44±0.10 and 0.40±0.07%, respectively). Besides starch analysis, proximate analyses (ash, moisture, lipid, protein, and carbohydrates) have been conducted. The results obtained indicated that all the cultures contain more than 4.50% of carbohydrates in average, followed by lipid and protein <1%. The results demonstrate that further optimization and various harvesting stages (early of exponential phase, early of stationary phase and late stationary phase) may increase lipid, carbohydrates, starch, and protein accumulation. Chlorella salina and Spirulina sp. will be used to further study on optimization of physical and chemical factors for high starch accumulation. Conclusion, significance and impact of study: In conclusion, this experiment focused more on preliminary screening for further application of starch uses in food and food packaging industries.

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Effects of fluctuating temperature and silicate supply on the growth, biochemical composition and lipid accumulation of Nitzschia sp.

Cited by 20 publications

References 35 publications

The eurythermal adaptivity and temperature tolerance of a newly isolated psychrotolerant Arctic Chlorella sp.

The eurythermal adaptivity and temperature tolerance of a newly isolated psychrotolerant Arctic Chlorella sp.

Screening of High Temperature-Tolerant Oleaginous Diatoms

Proximate and biochemical analysis for marine and freshwater algae

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