Effects of Fluoridated Milk on Root Dentin Remineralization

Arnold, Wolfgang H.; Heidt, Bastian A.; Kuntz, Sebastian; Naumova, Ella A.

doi:10.1371/journal.pone.0104327

Cited by 10 publications

(13 citation statements)

References 26 publications

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“…This is an intriguing question given that several other agents have been shown not only to do just that, but also to boost the fluoride-mediated inhibition of demineralization. These other agents include casein phosphopeptide-amorphous calcium phosphate [Hamba et al, 2011], Ca 3 SiO 5 [Wang et al, 2012], zinc [Lippert, 2012], strontium and aluminum [KoletsiKounari et al, 2012], tin [Rakhmatullina et al, 2013], milk [Arnold et al, 2014] and sodium trimetaphosphate [Manarelli et al, 2014].…”

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confidence: 99%

Synergistic Inhibition of Enamel Demineralization by Peptide 8DSS and Fluoride

Yang

Shi

et al. 2016

Caries Res

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The biomimetic peptide 8DSS has shown beneficial effects in promoting remineralization of demineralized enamel in vitro. Here we examined the ability of 8DSS alone and in combination with fluoride to inhibit enamel demineralization during pH-cycling mimicking intraoral conditions. Enamel blocks were subjected to 9 days of pH-cycling in the presence of 1,000 ppm NaF (positive control), distilled-deionized water (DDW; negative control), 25 μM 8DSS alone, 25 μM 8DSS with 500 ppm NaF (8DSS-FL) or 25 μM 8DSS with 1,000 ppm NaF (8DSS-FH) twice daily for 1 min each time. The blocks were analyzed in terms of surface microhardness (SMH), fluoride uptake and mineral content. The 8DSS-treated blocks showed significantly lower mineral loss, shallower lesions and higher SMH than the DDW-treated blocks. No significant differences were observed between the blocks treated with 8DSS alone or fluoride alone. The blocks treated with 8DSS alone or DDW showed similar amounts of fluoride uptake, which was the lowest of all the treatment groups. The blocks treated with 8DSS-FL or 8DSS-FH did not differ significantly, and both groups showed significantly greater SMH and fluoride uptake as well as significantly lower mineral loss and shallower lesions than the NaF-treated blocks. Mineral content was significantly higher in the 8DSS-treated blocks than in the DDW-treated blocks from the surface layer (10 µm) to the lesion depth (110 µm), and it was significantly higher in the blocks treated with 8DSS-FL or 8DSS-FH than in the NaF-treated blocks from 10 to 90 µm. These findings illustrate the potential of 8DSS for inhibiting enamel demineralization and for enhancing the anticaries effect of NaF.

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confidence: 99%

Synergistic Inhibition of Enamel Demineralization by Peptide 8DSS and Fluoride

Yang

Shi

et al. 2016

Caries Res

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“…The F concentrations in milk tested in studies involving dental caries range from 0.25 to 10.0 ppm [Giacaman et al, 2012;Malinowski et al, 2012b;Arnold et al, 2014;Lippert et al, 2014;Ongtenco et al, 2014]. In this study, the lowest concentration used was 2.5 ppm F due to the fact that the erosive process is more aggressive than the Superscript letters indicate significant differences among the treatments.…”

Section: Discussionmentioning

confidence: 94%

“…As a positive result of oral health prevention programmes, either through the ingestion of F from water, milk or the application of dental products, people started to retain their teeth in the mouth for a longer time compared with the past [Arnold et al, 2014]. Furthermore, with the advent of the industrialisation process and changes in lifestyle and eating habits, other oral diseases have become more common, such as, for example, tooth erosion.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Whole and Fat-Free Fluoridated Milk, Applied before or after Acid Challenge, against Dental Erosion

et al. 2016

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This study analysed in vitro the effect of milk against dental erosion, considering three factors: the type of milk (bovine whole/fat-free), the presence of different fluoride concentrations and the time of application (before/after erosive challenge). Bovine enamel (n = 15/group) and root dentine (n = 12/group) specimens were submitted to the following treatments: after the first erosive challenge - 0.9% NaCl solution (negative control), whole milk with 0, 2.5, 5.0 and 10.0 ppm F, fat-free milk with 0, 2.5, 5.0 and 10.0 ppm F, and 0.05% NaF solution (positive control); before the first erosive challenge - whole milk with 0, 2.5, 5.0 and 10.0 ppm F, fat-free milk with 0, 2.5, 5.0 and 10.0 ppm F, and 0.05% NaF solution (positive control). Specimens were submitted to demineralisation-remineralisation regimes 4 times/day for 5 days. The response variables were enamel and dentine loss (in micrometres). Data were analysed using Kruskal-Wallis/Dunn's test (p < 0.05). For enamel, whole milk containing 10 ppm F, applied before the erosive challenge, was the most protective treatment, but with no significant difference compared with the same treatment carried out after the erosive challenge. For dentine, whole fluoridated milk (all concentrations, after), fat-free 10 ppm F milk (after, before) and whole milk with or without F (except 2.5 ppm F, all before) significantly reduced dentine erosion. It seems that the presence of fluoride, especially at 10 ppm, is the most important factor in reducing dental erosion.

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“…The authors attributed this to the less mineralised nature of the dentinal tissue (Giacaman et al, 2012). The other study observed that fluoridated milk had a protective effect on root dentine remineralisation, but the effect of a sodium chloride solution was better (Arnold et al, 2014). In the present study, we employed dentine slabs with artificial caries lesions that are expected to contain a superficial layer of demineralised organic matrix (Buzalaf, Kato, & Hannas, 2012;Tjaderhane et al, 1998).…”

Section: Treatmentmentioning

confidence: 90%

“…Regarding dentine, an in vitro study analysed the remineralising effect of different solutions (sodium chloride, artificial saliva, milk, milk + 2.5 ppm fluoride, milk + 10 ppm fluoride and artificial saliva + 10 ppm fluoride). The results showed a positive effect of the fluoridated milk, but the group treated with sodium chloride presented a better effect (Arnold, Heidt, Kuntz, & Naumova, 2014). However, only one in situ study evaluated the influence of different fluoride concentrations in milk on enamel remineralisation, but the quantities of fluoride used added in milk (1.5 or 3.0 mg) were higher than those typically employed in milk fluoridation schemes and the authors evaluated only surface rehardening (Lippert, Martinez-Mier, & Zero, 2014).…”

Section: Introductionmentioning

confidence: 99%