Effects of Fluoride and Aluminum from Ionomeric Materials on S. mutans Biofilm

Abstract

Ionomeric materials release different proportions of fluoride and aluminum. Their simultaneous effect on the acidogenicity and composition of S. mutans biofilm is unknown. Six cylindrical specimens of each material (Ketac-fil, Vitremer, Fuji-Ortho LC, F-2000, and Z-100) were incubated with S. mutans GS-5 in culture media containing 5% sucrose (w/v). The media were changed daily for seven days, during which the pH and concentrations of fluoride and aluminum were determined. Furthermore, the concentrations of these ions and insoluble polysaccharide were determined in the biofilm formed at the end of the experimental period. The results showed that all the materials tested released fluoride. However, Vitremer released the highest amount of aluminum and was the most effective in reducing the acidogenicity of S. mutans biofilms. It also significantly affected both biofilm formation and composition. Thus, this study suggests that aluminum released by ionomeric materials may enhance the biological effects of fluoride.

Surface modification of stainless steel by plasma-based fluorine and silver dual ion implantation and deposition

“…The metal-fluoride complexes are also responsible for fluoride inhibition of protontranslocating F-ATPases and are thought to mimic phosphate to form complexes with ADP at the reaction center of enzymes. Indeed, ATPase plays an important role in the maintenance of intracellular pH by pumping out protons and in the inhibition of the enzyme that disrupts the bacterial metabolism and aciduric capability of Streptococcus mutans 17,[36][37][38] .…”

Real-Time Monitoring of <i>Streptococcus mutans</i> Biofilm Formation Using a Quartz Crystal Microbalance

“…To monitor the biofilm development and growth of S. mutans , previous techniques have required sampling of the biofilm from the support medium by sonication or physical means for measuring cell count and viability [Lee et al, 1996], or dry weight measurement and chemical analysis [Hayacibara et al, 2003]. However, in situ methods such as electrochemical QCM and QCM, which have been used to investigate other micro-organisms such as mixed cultures from drinking water biofilms [Bressel et al, 2003] and P. cepacia [Nivens et al, 1993], would be beneficial.…”

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

“…The antimicrobial nature of these cements is predominantly due to the release of the fluoride ion (F -) [9][10][11] and also related to setting occuring at a low pH [12]. GPCs have the additional ability to uptake and re-release F -ions in a F -rich aqueous environment, which can impart a prolonged antibacterial response [10].…”