Effects of fluoroquinolones and tetracyclines on mitochondria of human retinal MIO-M1 cells

Salimiaghdam, Nasim; Singh, Lata; Schneider, Kevin; Chwa, Marilyn; Atilano, Shari R.; Nalbandian, Angèle; Limb, G. Astrid; Kenney, M. Cristina

doi:10.1016/j.exer.2021.108857

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Furthermore, overexpression of apoptotic (CASP-7, CASP-9 and BAX) and inflammatory (IL-6) pathway genes was induced by exposure to CPFX [28]. Moreover, our recent study performed on MIO-M1 (human retinal Müller) cells treated with CPFX and TETRA showed CPFX induced lower levels of cellular metabolism, ∆ψM and ROS along with overexpression of CASP-3 and CASP-9 [29].…”

Section: Introductionmentioning

confidence: 90%

Impacts of Bacteriostatic and Bactericidal Antibiotics on the Mitochondria of the Age-Related Macular Degeneration Cybrid Cell Lines

Salimiaghdam

Singh

et al. 2022

Biomolecules

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We assessed the potential negative effects of bacteriostatic and bactericidal antibiotics on the AMD cybrid cell lines (K, U and J haplogroups). AMD cybrid cells were created and cultured in 96-well plates and treated with tetracycline (TETRA) and ciprofloxacin (CPFX) for 24 h. Reactive oxygen species (ROS) levels, mitochondrial membrane potential (ΔψM), cellular metabolism and ratio of apoptotic cells were measured using H2DCFDA, JC1, MTT and flow cytometry assays, respectively. Expression of genes of antioxidant enzymes, and pro-inflammatory and pro-apoptotic pathways were evaluated by quantitative real-time PCR (qRT-PCR). Higher ROS levels were found in U haplogroup cybrids when treated with CPFX 60 µg/mL concentrations, lower ΔψM of all haplogroups by CPFX 120 µg/mL, diminished cellular metabolism in all cybrids with CPFX 120 µg/mL, and higher ratio of dead cells in K and J cybrids. CPFX 120 µg/mL induced overexpression of IL-33, CASP-3 and CASP-9 in all cybrids, upregulation of TGF-β1 and SOD2 in U and J cybrids, respectively, along with decreased expression of IL-6 in J cybrids. TETRA 120 µg/mL induced decreased ROS levels in U and J cybrids, increased cellular metabolism of treated U cybrids, higher ratio of dead cells in K and J cybrids and declined ΔψM via all TETRA concentrations in all haplogroups. TETRA 120 µg/mL caused upregulation of IL-6 and CASP-3 genes in all cybrids, higher CASP-7 gene expression in K and U cybrids and downregulation of the SOD3 gene in K and U cybrids. Clinically relevant dosages of ciprofloxacin and tetracycline have potential adverse impacts on AMD cybrids possessing K, J and U mtDNA haplogroups in vitro.

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Section: Introductionmentioning

confidence: 90%

Impacts of Bacteriostatic and Bactericidal Antibiotics on the Mitochondria of the Age-Related Macular Degeneration Cybrid Cell Lines

Salimiaghdam

Singh

et al. 2022

Biomolecules

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“…Ciprofloxacin is one of the fluoroquinolones currently used as bactericidal antibiotics. It up-regulates BCL-RAMBO expression at the transcriptional level, and this is accompanied by an increase in caspases 3 and 9 in human adult retinal pigment epithelial-19 (ARPE-19) cells and human Müller MIO-M1 cells (Salimiaghdam et al, 2020;Salimiaghdam et al, 2022). Anti-VEGF drugs (i.e., afibercept, ranibizumab, and bevacizumab) also upregulate the mRNA expression of BCL-RAMBO, together with caspases 3 and 9, in ARPE-19 cells and MIO-M1 cells (Cáceresdel-Carpio et al, 2020;Hamid et al, 2021).…”

Section: Transcriptional Regulation Of Bcl-rambo By Miscellaneous Exp...mentioning

confidence: 99%

Biological properties of the BCL-2 family protein BCL-RAMBO, which regulates apoptosis, mitochondrial fragmentation, and mitophagy

Kataoka

2022

Front. Cell Dev. Biol.

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Mitochondria play an essential role in the regulation of cellular stress responses, including cell death. Damaged mitochondria are removed by fission and fusion cycles and mitophagy, which counteract cell death. BCL-2 family proteins possess one to four BCL-2 homology domains and regulate apoptosis signaling at mitochondria. BCL-RAMBO, also known as BCL2-like 13 (BCL2L13), was initially identified as one of the BCL-2 family proteins inducing apoptosis. Mitophagy receptors recruit the ATG8 family proteins MAP1LC3/GABARAP via the MAP1LC3-interacting region (LIR) motif to initiate mitophagy. In addition to apoptosis, BCL-RAMBO has recently been identified as a mitophagy receptor that possesses the LIR motif and regulates mitochondrial fragmentation and mitophagy. In the 20 years since its discovery, many important findings on BCL-RAMBO have been increasingly reported. The biological properties of BCL-RAMBO are reviewed herein.

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Antibiotics modulate frequency and early generation of epileptic seizures in zebrafish

Sivarajan

Ramachandran

2023

Exp Brain Res

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Effects of fluoroquinolones and tetracyclines on mitochondria of human retinal MIO-M1 cells

Cited by 6 publications

References 41 publications

Impacts of Bacteriostatic and Bactericidal Antibiotics on the Mitochondria of the Age-Related Macular Degeneration Cybrid Cell Lines

Impacts of Bacteriostatic and Bactericidal Antibiotics on the Mitochondria of the Age-Related Macular Degeneration Cybrid Cell Lines

Biological properties of the BCL-2 family protein BCL-RAMBO, which regulates apoptosis, mitochondrial fragmentation, and mitophagy

Antibiotics modulate frequency and early generation of epileptic seizures in zebrafish

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