Effects of focused ultrasound and dry needling on tendon mechanical properties

Khandare, Sujata; Smallcomb, Molly; Butt, Ali A.; Elliott, Jacob C.; Simon, Julianna C.; Vidt, Meghan E.

doi:10.1016/j.jbiomech.2021.110934

Cited by 2 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tendon mechanical properties might further improve given longer recovery times. Outcomes of our current study are consistent with our previous study 19 in ex vivo rat tendons, suggesting that fUS preserves tendon mechanical properties better than DN. Given that fUS could noninvasively trigger a similar inflammatory response from controlled microdamage, and preserve mechanical properties in tendons, this study suggests that fUS should be studied further to determine how this modality can be leveraged as a therapy for tendinopathies.…”

Section: Discussionsupporting

confidence: 91%

“…For fUS, a spherically curved single‐element fUS transducer of 1.5 MHz operating frequency and f‐number of 0.7 (H‐234 modified with 42‐mm central opening; Sonic Concepts) and linear radiofrequency power amplifier (55 dB, 1040 L; Electronics & Innovations Ltd.) were used to deliver 1 ms pulses at 10 Hz for 106 s with peak positive and negative pressures of 49 and 19 MPa, respectively, while the rat was partially submerged in a degassed, deionized water tank (Figure 1). These parameters were based on our previous ex vivo parameter exploration studies 19,20 . Focal dimensions, or full widths at half maximum pressures, were measured with a fiber optic probe hydrophone (HFO‐690; ONDA) to be 10.5 mm axially and 1 mm transversely for a peak negative pressure of 19 MPa (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…Tendons were mechanically tested following procedures described previously 19,24 . Briefly, width and thickness were measured from the central region of the tendon using digital Vernier calipers and tendon area was calculated assuming elliptical cross‐section (Table 2).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Focused ultrasound as an alternative to dry needling for the treatment of tendinopathies: A murine model

Khandare,

Smallcomb,

Elliott

et al. 2023

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Tendinopathies account for 30% of 102 million annual musculoskeletal injuries occurring annually in the United States. Current treatments, like dry needling, induce microdamage to promote healing but produce mixed success rates. Previously, we showed focused ultrasound can noninvasively create microdamage while preserving mechanical properties in ex vivo murine tendons. This present study compared growth factor, histological, and mechanical effects after focused ultrasound or dry needling treatments in an in vivo murine tendon injury model. Partial Achilles tenotomy was performed in 26 rats. One‐week postsurgery, tendons were treated with focused ultrasound (1.5 MHz, 1‐ms pulses at 10 Hz for 106 s, p+ = 49 MPa, p− = 19 MPa) or dry needling (30 G needle, 5 fenestrations over 20 s) and survived for 1 additional week. Blood was collected immediately before and after treatment and before euthanasia; plasma was assayed for growth factors. Treated tendons and contralateral controls were harvested for histology or mechanical testing. No differences were found between treatments in release of insulin growth factor 1 and transforming growth factor beta; vascular endothelial growth factor A concentrations were too low for detection. Histologically, focused ultrasound and dry needling tendons displayed localized fibroblast infiltration without collagen proliferation with no detectable differences between treatments. Mechanically, stiffness and percent relaxation of dry needling tendons were lower than controls (p = 0.0041, p = 0.0441, respectively), whereas stiffness and percent relaxation of focused ultrasound tendons were not different from controls. These results suggest focused ultrasound should be studied further to determine how this modality can be leveraged as a therapy for tendinopathies.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Focused ultrasound as an alternative to dry needling for the treatment of tendinopathies: A murine model

Khandare,

Smallcomb,

Elliott

et al. 2023

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Focused ultrasound treatments were applied for 1 or 5 min. Bubble activity and transducer alignment were monitored coaxially with B-mode ultrasound imaging using a Verasonics research ultrasound system (Vantage-128, Kirkland, WA) and a P4-2 imaging transducer (Philips/ATL, Bothell, WA) . The focus was aligned 1.5 cm in depth at one center location in the gel.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Bubble activity and transducer alignment were monitored coaxially with Bmode ultrasound imaging using a Verasonics research ultrasound system (Vantage-128, Kirkland, WA) and a P4-2 imaging transducer (Philips/ATL, Bothell, WA). 38 The focus was aligned 1.5 cm in depth at one center location in the gel. A diagram and pictures of the setup used for focused ultrasound are available in the Supporting Information (Figures S1 and S2).…”

Section: ■ Introductionmentioning

confidence: 99%

Ultrasound-Responsive Hydrogels for On-Demand Protein Release

Arrizabalaga

Smallcomb

Abu‐Laban

et al. 2022

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

The development of tunable, ultrasound-responsive hydrogels that can deliver protein payload on-demand when exposed to focused ultrasound is described in this study. Reversible Diels−Alder linkers, which undergo a retro reaction when stimulated with ultrasound, were used to cross-link chitosan hydrogels with entrapped FITC-BSA as a model protein therapeutic payload. Two Diels−Alder linkage compositions with large differences in the reverse reaction energy barriers were compared to explore the influence of linker composition on ultrasound response. Selected physicochemical properties of the hydrogel construct, its basic degradation kinetics, and its cytocompatibility were measured with respect to Diels−Alder linkage composition. Focused ultrasound initiated the retro Diels− Alder reaction, controlling the release of the entrapped payload while also allowing for real-time visualization of the ongoing process. Additionally, increasing the focused ultrasound amplitude and time correlated with an increased rate of protein release, indicating stimuli responsive control.

show abstract

Effects of focused ultrasound and dry needling on tendon mechanical properties

Cited by 2 publications

References 35 publications

Focused ultrasound as an alternative to dry needling for the treatment of tendinopathies: A murine model

Focused ultrasound as an alternative to dry needling for the treatment of tendinopathies: A murine model

Ultrasound-Responsive Hydrogels for On-Demand Protein Release

Contact Info

Product

Resources

About