Effects of food contaminated with cadmium and copper on hemocytes of Steatoda grossa (Araneae: Theridiidae)

Wilczek, Grażyna; Wiśniewska, Kamila; Kozina, Bartosz; Wilczek, Piotr; Rost‐Roszkowska, Magdalena; Stalmach, Monika; Skowronek, Magdalena; Kaszuba, F.

doi:10.1016/j.ecoenv.2017.12.007

Cited by 20 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Babczynska et al (2011b) revealed that Agelena labyrinthica collected from previously contaminated sites significantly accumulated higher amount of Cu compared with control when fed with metal contaminated diet in the laboratory. Also, bioaccumulation of Cu in Steatoda grossa fed with metal administrated prey were more than two times higher compared with control in a four weeks period (Wilczek et al 2018).…”

Section: Single Metal Exposurementioning

confidence: 84%

“…Previous studies on spiders have examined the toxic effect of metals, such as Pb and Cu singly, through either metal-contaminated food or water (Jung et al 2005;Babczynska et al 2011b;Wilczek et al 2018). The exposure to multiple metals may induce synergistic, antagonistic or additive toxicological effects which may be different from single metal exposure (Flouty and Khalaf 2015;Wijayawardena et al 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes

Aziz

Butt

Elsheikha

2019

Environ Sci Pollut Res

View full text Add to dashboard Cite

There are major concerns regarding the environmental and human health risks caused by exposure to heavy metals. Spiders are often used as a model in ecotoxicological studies to assess soil pollution. In present study, we measured the bioaccumulation of copper (Cu) and lead (Pb) in spiders, Lycosa terrestris and Pardosa birmanica by ICP-MS. We determined whether Cu and Pb accumulation differed in (i) different spider species, (ii) single compared to combined exposure, and (iii) routes of exposure. Spiders were exposed to 10mM CuSO4 and 10mM PbCl2 solutions separately or combined (10mM+10mM) through different exposure routes i.e. spiked soil and food for 6 weeks. Effect of metals on survival and body mass of exposed and unexposed (control) spiders was determined. In both spider species, accumulation of metals increased with exposure time. In single metal exposure, Cu accumulation from food was higher than soil exposure in both spiders species, whereas the opposite was observed for Pb. In combined treatment, the uptake of both metals significantly decreased from similar accumulation routes. L. terrestris accumulated higher concentration of metals than P. birmanica during soil exposure. Metal exposure via contaminated food caused higher mortality compared to soil exposure. Body mass of both spider species was significantly decreased and negatively correlated with metal's concentration. These results suggest that bioaccumulation efficiency of Cu and Pb differs significantly in spiders exposed to metal's mixture compared to a single metal exposure, and is dependent on exposure route, type of metal and spider species.More understanding of the effect of exposure to metal mixture and exposure routes may help to improve risk assessment and ecological monitoring programs.

show abstract

Section: Single Metal Exposurementioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes

Aziz

Butt

Elsheikha

2019

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…They also explained this result by the fact that hemocytes play a crucial role in animal immunity and defense against pathogens. Additionally, for the same reasons, hemocytes were used as markers of the condition of spiders exposed to cadmium 51 . Moreover, in spiders, hemocytes appeared to be more sensitive to cadmium toxicity than midgut cells, based on the measurement of DNA damage levels 52 .…”

Section: Discussionmentioning

confidence: 99%

Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment

Babczyńska

Nowak

Kafel

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Autophagy is a natural process that aims to eliminate malfunctioning cell parts, organelles or molecules under physiological conditions. It is also induced in response to infection, starvation or oxidative stress to provide energy in case of an energy deficit. The aim of this 2-dimensional study was to test if, and if so, how, this process depends on the concentration of cadmium in food (with Cd concentrations from 0 to 352 μg of Cd per g of food (dry weight)—D1 dimension) and the history of selection pressure (160 vs 20 generations of exposure to Cd—D2 dimension). For the study, the 5th instar larvae of a unique strain of the moth Spodoptera exigua that was selected for cadmium tolerance for 160 generations (44 μg of Cd per g of food (dry weight)), as well as 20-generation (11, 22 and 44 μg of Cd per g of food (dry weight)) and control strains, were used. Autophagy intensity was measured by means of flow cytometry and compared with life history parameters: survivability and duration of the 3rd larval stage. The highest values of autophagy markers were found in the groups exposed to the highest Cd concentration and corresponded (with a significant correlation coefficient) to an increased development duration or decreased survivorship in the respective groups. In conclusion, autophagy is probably initiated only if any other defense mechanisms, e.g., antioxidative mechanisms, are not efficient. Moreover, in individuals from pre-exposed populations, the intensity of autophagy is lower.

show abstract

“…Spiders at every stage of ontogenesis are constantly exposed to environmental stress. The environmental factors that stimulate the immune system of invertebrates include, among others, injuries ( Taylor, Roberts & Uetz, 2006 ), pathogen infections ( Fukuzawa et al, 2008 ), parasitoid attacks ( Schmidt, 2008 ) or the chemicals such as heavy metals ( Wilczek et al, 2018 ) and pesticides ( Laino et al, 2021 ). Due to such a substantial evolutionary success that the representatives of the Araneae order have achieved, their immune system must be able to respond to various types of immunostimulants and may have mechanisms to counteract the simultaneous action of several immune stimulants at once.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulation of Parasteatoda tepidariorum (Araneae: Theridiidae) in juvenile and adult stages. Immunity reactions to injury with foreign body and Bacillus subtilis infection

Czerwonka¹,

Sawadro²,

Brożek³

et al. 2023

PeerJ

View full text Add to dashboard Cite

To assess the immune potential of spiders, in the present study juvenile and adult females of Parasteatoda tepidariorum were exposed to Bacillus subtilis infection, injury by a nylon monofilament and a combination of both. The expression level of selected immune-related genes: defensin 1 (PtDEF1), lysozyme 1 (PtLYS1), lysozyme C (PtLYSC), lysozyme M1 (PtLYSM1), autophagy-related protein 101 (PtATG101), dynamin (PtDYN) and heat shock proteins (HSP70) (PtHSPB, PtHSPB2A, PtHSPB2B), production of lysozyme and HSP70 proteins, and hemocytes viability were measured. The obtained results indicated expression of the lysozyme, autophagy-related protein and HSP70 genes in both ontogenetic stages of P. tepidariorum. It has been also shown that the simultaneous action of mechanical and biological factors causes higher level of lysozyme and HSP70, cell apoptosis intensity and lower level of hemocytes viability than in the case of exposure to a single immunostimulant. Moreover, mature females showed stronger early immune responses compared to juveniles.

show abstract

Effects of food contaminated with cadmium and copper on hemocytes of Steatoda grossa (Araneae: Theridiidae)

Cited by 20 publications

References 37 publications

Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes

Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes

Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment

Immunostimulation of Parasteatoda tepidariorum (Araneae: Theridiidae) in juvenile and adult stages. Immunity reactions to injury with foreign body and Bacillus subtilis infection

Contact Info

Product

Resources

About