Effects of formalin fixation on tissue optical polarization properties

Wood, Michael L.; Vurgun, N; Wallenburg, Marika A.; Vitkin, I. Alex

doi:10.1088/0031-9155/56/8/n01

Cited by 35 publications

(38 citation statements)

References 12 publications

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“…Small fixation effects were expected since immersion of the section in fixative may cause slight tissue displacement, deformation and shrinkage [42].…”

Section: Discussionmentioning

confidence: 99%

“…Formalin fixation has been shown to affect optical polarization properties of myocardium and liver samples [42], and therefore fixation could affect our measurements in ocular tissues. To quantify the effects of formalin fixation on PLM-measured fiber orientation we used the following experiment: a fresh sheep eye was cryosectioned sagittally into 30 µm thick sections.…”

Section: Robustness To Tissue Fixationmentioning

confidence: 99%

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Polarization microscopy for characterizing fiber orientation of ocular tissues

Jan

Grimm

Tran

et al. 2015

Biomed. Opt. Express

150

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Characterizing the collagen fiber orientation and organization in the eye is necessary for a complete understanding of ocular biomechanics. In this study, we assess the performance of polarized light microscopy to determine collagen fiber orientation of ocular tissues. Our results demonstrate that the method provides objective, accurate, repeatable and robust data on fiber orientation with µm-scale resolution over a broad, cmscale, field of view, unaffected by formalin fixation, without requiring tissue dehydration, labeling or staining. Together, this shows that polarized light microscopy is a powerful method for studying collagen architecture in the eye, with applications ranging from normal physiology and aging, to pathology and transplantation.

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“…Small fixation effects were expected since immersion of the section in fixative may cause slight tissue displacement, deformation and shrinkage [42].…”

Section: Discussionmentioning

confidence: 99%

Section: Robustness To Tissue Fixationmentioning

confidence: 99%

Polarization microscopy for characterizing fiber orientation of ocular tissues

Jan

Grimm

Tran

et al. 2015

Biomed. Opt. Express

150

View full text Add to dashboard Cite

show abstract

“…Samples were moistened periodically with phosphate-buffered saline solution (0.02 M) to maintain freshness; this avoids potential complications associated with formalin fixation and other tissue handling or preparation methods. 18 Three previously reported low-birefringence (∼isotropic) tissues (liver, kidney cortex, and brain) and three highly birefringent (anisotropic) tissues (myocardium muscle, loin muscle, and tendon) were chosen. Liver was selected as an isotropic and highly absorbent tissue.…”

Section: Methodsmentioning

confidence: 99%

Quantitative correlation between light depolarization and transport albedo of various porcine tissues

et al. 2012

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Abstract. We present a quantitative study of depolarization in biological tissues and correlate it with measured optical properties (reduced scattering and absorption coefficients). Polarized light imaging was used to examine optically thick samples of both isotropic (liver, kidney cortex, and brain) and anisotropic (cardiac muscle, loin muscle, and tendon) pig tissues in transmission and reflection geometries. Depolarization (total, linear, and circular), as derived from polar decomposition of the measured tissue Mueller matrix, was shown to be related to the measured optical properties. We observed that depolarization increases with the transport albedo for isotropic and anisotropic tissues, independent of measurement geometry. For anisotropic tissues, depolarization was higher compared to isotropic tissues of similar transport albedo, indicating birefringence-caused depolarization effects. For tissues with large transport albedos (greater than ∼0.97), backscattering geometry was preferred over transmission due to its greater retention of light polarization; this was not the case for tissues with lower transport albedo. Preferential preservation of linearly polarized light over circularly polarized light was seen in all tissue types and all measurement geometries, implying the dominance of Rayleigh-like scattering. The tabulated polarization properties of different tissue types and their links to bulk optical properties should prove useful in future polarimetric tissue characterization and imaging studies.

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“…The tissue scattering coefficient (μ s ) is relatively lager than the values previously reported [75,76]. This increase may originate from formalin fixation since it has been reported that formalin fixation can result in increasing scattering coefficient due to the cross-linking of proteins creating a more highly scattering media [77,78]. …”

Section: Parameters For Tumor Detectionmentioning

confidence: 81%

Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

Tang

Wang

Frank

et al. 2016

Biomed. Opt. Express

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Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc Min /J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization. Muthasamy, C. J. Lightdale, N. Santiago, D. K. Pleskow, P. J. Dean, and K. K. Wang, "Endoscopic radiofrequency ablation for Barrett's esophagus: 5-year outcomes from a prospective multicenter trial," Endoscopy 42(10), 781-789 (2010 cancer lesion with swept-source optical coherence tomography," J.

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Effects of formalin fixation on tissue optical polarization properties

Cited by 35 publications

References 12 publications

Polarization microscopy for characterizing fiber orientation of ocular tissues

Polarization microscopy for characterizing fiber orientation of ocular tissues

Quantitative correlation between light depolarization and transport albedo of various porcine tissues

Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

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