Effects of formalin on haematological and blood chemistry in olive flounder,<i>Paralichthys olivaceus</i>(Temminck et Schlegel)

Jung, Sung Hee; Sim, Doo Saing; Park, Mi-Seon; Jo, Qtae; Kim, Yoon

doi:10.1046/j.1365-2109.2003.00936.x

Cited by 47 publications

(29 citation statements)

References 18 publications

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“…1). Jung et al (2003) não observaram alterações no quadro leucocitário do linguado (Paralichthys olivaceus) quando este foi submetido a banhos de uma hora com diferentes concentrações de formalina (0,1, 0,2 e 0,3 ml L -1 ), porém observou que o formol pode causar inibição na transferência de oxigênio do sangue e danos no fígado e rim quando estes foram submetidos a banhos de curta duração. Para larvas de trairão (Hoplias lacerdae) a formalina foi considerada moderadamente tóxica com concentração letal (CL 50 ) em 96 horas de 0,2 ml L -1 e em concentrações de 0,1 a 0,2 ml L -1…”

Section: Resultsunclassified

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Efeitos do tratamento com formalina e sulfato de cobre sobre os parâmetros hematológicos e parasitos monogenéticos em juvenis de Hemigrammus sp. (Osteichthyes: Characidae)

et al. 2013

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RESUMOO objetivo deste trabalho foi avaliar a eficácia da formalina e sulfato de cobre no controle de monogenéticos em juvenis de Hemigrammus sp e seu efeito na contagem diferencial de leucócitos. Utilizou-se um delineamento inteiramente casualizado com 5 tratamentos e 3 repetições. Todos os tratamentos tiveram duração de três dias e corresponderam a: T1, controle; T2 e T3 banho por imersão de uma hora em formalina, em duas concentrações uma de 0,25 ml e outra 0,1 ml formalina L ). Após o período experimental observou-se que em T2 ocorreu 100% de mortalidade. O T3 e T5 proporcionaram eficácia de 100%, porém os peixes apresentaram intoxicação e mortalidades de 66 e 80%, respectivamente. T4 apresentou eficácia de 77,7% e diminuição dos índices parasitológicos: número total de parasitos de 11,3±9, intensidade média de infecção de 3,2±2 e prevalência de 47,1%. Quanto aos leucócitos observou-se que no T3, T4 e T5 ocorreram diferenças na proporção de linfócitos, monócitos e neutrófilos em relação ao controle. O CuSO 4 e a formalina reduzem a carga parasitária, mas apresentaram-se tóxicos nas maiores concentrações. PALAVRAS-CHAVE: CuSO 4 , formol, monogênias, tratamento Evaluation of formalin and copper sulfate on monogenetic parasite (Urocleidoides sp.) of Hemigrammus sp. (Osteichthyes: Characidae) juveniles ABSTRACTThe objective of this study was to evaluate the effectiveness of formalin and copper sulphate to control monogeneans in juvenile Hemigrammus sp and its effect on leukocyte count. We used a completely randomized design with five treatments and three replications. All treatments lasted for three days and consisted of: T1, control; T2 and T3 immersion bath for one hour in formalin, an of two concentrations of 0.25 ml formalin and the other 0.1 ml L ). After the experimental period was observed that T2, 100% mortality. The T3 and T5 provided 100% efficiency, however, fish presented mortalities and intoxication of the 66 and 80% respectively. T4 showed an efficacy of 77.7% and decrease in parasitological indices: total number of parasites of11, 3 ± 9, mean infection intensity of 3.2 ± 2 and a prevalence of 47.1%. As the leukocytes was observed that at T3, T4 and T5 occurred differences in the proportion of lymphocytes, monocytes and neutrophils as compared to control. The CuSO 4 and formalin reduce the parasite load, but presented to be toxic at higher concentrations.

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Section: Resultsunclassified

“…Assim, o monitoramento das respostas hematológicas se apresenta como uma alternativa, pois, Jung et al (2003) afirmaram que os valores sanguíneos demostram a toxicidade de um produto químico. Porém, não existe uma padronização, para os peixes tropicais, do quadro hematológico o que dificulta a interpretação de resultados.…”

Section: Introductionunclassified

Efeitos do tratamento com formalina e sulfato de cobre sobre os parâmetros hematológicos e parasitos monogenéticos em juvenis de Hemigrammus sp. (Osteichthyes: Characidae)

et al. 2013

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“…Chemicals. The chemical treatments were a daily bath in 200 ppm of formalin (Marine Depot) (Jung et al 2003) or 200 ppm of hydrogen peroxide (Russo et al 2007) in a daily bath or dip treatment for 10 min after challenge with live Aeromonas hydrophila.…”

Section: Methodsmentioning

confidence: 99%

Effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with Aeromonas hydrophila

Harikrishnan¹,

Balasundaram²,

Heo³

2009

Dis. Aquat. Org.

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We report the effect of various chemicals, vaccines and immunostimulants on innate immune mechanisms such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity and disease resistance in goldfish Carassius auratus (23 ± 2 g) against Aeromonas hydrophila on Days 15 and 30 post-infection. In infected fish, the administration of diets supplemented with either probiotics, tri-herbal extract or azadirachtin for 30 d significantly enhanced phagocytic activity; administration for 15 d had no effect. In fish treated with heat-killed and formalin-killed vaccines, and probiotics-, tri-herbal-and azadirachtin-supplemented diets, superoxide anion production was enhanced on Day 30. However, there was no superoxide anion production in fish treated with tetracycline, furanace, formalin or hydrogen peroxide. Complement activity was also significantly enhanced on Days 15 and 30 in fish groups treated with vaccine and probiotics-, tri-herbal-and azadirachtin-supplemented diets. Other groups had no complement activity. Lysozyme activity was significantly enhanced after 30 d in fish treated with heat-killed vaccine and probiotics-, tri-herbal-and azadirachtin-supplemented diets. The probiotics-and tri-herbalcontaining diet administration groups had no mortality (0%) preceding the challenge with live A. hydrophila. Groups treated with heat-or formalin-killed vaccines or azadirachtin-supplemented diet suffered mortalities of 30, 35 and 10%, respectively, which represents an improvement of survival rate compared to untreated infected control groups. We conclude that probiotics-, tri-herbaland azadirachtin-supplemented diets act as immunostimulants enhancing the goldfish innate immune response and disease resistance against A. hydrophila. KEY WORDS: Nonspecific immune parameter · Carassius auratus · Aeromonas hydrophila · Tri-herbal · Probiotic · Azadirachtin Resale or republication not permitted without written consent of the publisherDis Aquat Org 88: [45][46][47][48][49][50][51][52][53][54] 2009 (Areerat 1987) and other regions of the world (Amin et al. 1985). A. hydrophila routinely isolated from healthy and moribund fish produce cytotoxins, proteases, surface (S)-layers and aerolysin toxins (Cahill 1990, Paniagua et al. 1990, Rahman et al. 1997.Aeromonas hydrophila infection can be controlled with antibiotics and chemicals. Traditional use of oral antibiotics such as oxytetracycline, chloramphenicol and nifurpirinol remains the most convenient form of therapy for motile aeromonad septicaemia (Noga 1996). However, antibiotic treatment is costprohibitive to farmers in many undeveloped and developing countries. Extensive use of antibiotics may result in residues (Shao 2001) and an increase in multidrug-resistant bacteria in human and veterinary medicine (Bryan 1984). Therefore, vaccination is a better choice to control A. hydrophila infections. At present, no vaccines for the protection of farmed fish against A. hydrophila infections are commercially av...

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“…In the present study, the serum ALP activity also varied in accordance with the fish SGR as potassium level increased, which further suggests that serum ALP would be related to fish growth and bone mineralization. The activities of AST and ALT of serum have been used to evaluate the liver damage of fish Jung et al, 2003). When liver damage occurred, intracellular ALT and AST would leak into the serum, which elevated the serum AST and ALT values.…”

Section: Tablementioning

confidence: 99%

Quantifying the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus)

Zhu¹,

Liu²,

Chen³

et al. 2014

Aquaculture

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A growth trial lasting for 12 weeks was conducted in a semi flow-through system to determine the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus) with an initial body weight of 4.8 g. Seven isonitrogenous and isoenergetic semi-purified diets were compounded with different dietary potassium levels: 1. 50, 2.32, 3.28, 5.30, 7.12, 9.45 and 10.44 g kg −1 diet. The results showed that specific growth rate and feed efficiency of grass carp were significantly (P b 0.05) affected by dietary potassium level, the highest value of specific growth rate was in 5.30 g kg −1 group and feed efficiency values in 5.30, 7.12 and 9.45 g kg −1 groups were significantly higher than those in the other groups. The survival rate of grass carp in the present study was not affected by dietary potassium level (P N 0.05). The Na + -K + ATPase activity value in the gills varied significantly (P b 0.05) with dietary potassium level and showed an increasing and then decreasing trend, and the highest value was in the 7.12 g kg −1 group. Dietary potassium level showed significant (P b 0.05) effect on serum SOD, ALP and AST activities. SOD and ALP activities increased and then decreased as potassium level increased, while AST decreased first and then increased. Serum TP and TC contents increased and then decreased with potassium level (P b 0.05). TG, ALT and BUN were not affected by dietary potassium level (P N 0.05). Body potassium and moisture contents increased and lipid content decreased with dietary potassium content, while ash content in fish body increased first and then decreased as potassium content increased (P b 0.05). Body protein in the 10.44 g kg −1 group was significantly lowered (P b 0.05). However, calcium, magnesium, phosphorus and sodium contents in dry matter were not affected by dietary potassium level. When the potassium content of water was 1.86-3.10 mg L −1 , judging from the polynomial regression analysis for the specific growth rate of fish and the Na + -K + ATPase activity in the gill, and the broken-line analysis for body potassium content, the optimal dietary potassium content for juvenile grass carp was 4.65, 7.27 and 5.98 g kg −1 diet respectively.

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Effects of formalin on haematological and blood chemistry in olive flounder,Paralichthys olivaceus(Temminck et Schlegel)

Cited by 47 publications

References 18 publications

Efeitos do tratamento com formalina e sulfato de cobre sobre os parâmetros hematológicos e parasitos monogenéticos em juvenis de Hemigrammus sp. (Osteichthyes: Characidae)

Efeitos do tratamento com formalina e sulfato de cobre sobre os parâmetros hematológicos e parasitos monogenéticos em juvenis de Hemigrammus sp. (Osteichthyes: Characidae)

Effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with Aeromonas hydrophila

Quantifying the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus)

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