Effects of Free Sterols, Steryl Ester, and Steryl Glycoside on Membrane Permeability

Grünwald, C.

doi:10.1104/pp.48.5.653

Cited by 152 publications

(88 citation statements)

References 15 publications

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“…He has further established that a free C3 hydroxyl group, at least one double bond, and a relatively flat configuration are requirements for the membrane activity and, specifically, that sterol esters and glycosides are inactive [5,12]. A comparison of the activity of cholesterol, campersterol, stigmasterol and /I-sitosterol seems to indicate that it decreases as the bulkiness of the Ci, side chain increases [ 121.…”

Section: Discussionmentioning

confidence: 99%

“…Sterols are considered structural components of higher plant membranes on the basis of their presence in membrane-containing subcellular fractions [l-3] , their effect on plant membrane permeability when added exogenously [4,5] and the sensitivity of plant cells to the polyene antibiotic filipin [6-81, which action is known to depend on the presence of sterols in the membrane (see [8]). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of n‐butanol and filipin on membrane permeability of developing wheat endosperms with different sterol phenotypes

Carbonero¹,

Torres²,

Garcı́a-Olmedo³

1975

FEBS Letters

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of n‐butanol and filipin on membrane permeability of developing wheat endosperms with different sterol phenotypes

Carbonero¹,

Torres²,

Garcı́a-Olmedo³

1975

FEBS Letters

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“…Each fraction was taken to dryness under reduced pressure. Sterol analysis of each fraction was by gas chromatography (4,5).…”

mentioning

confidence: 99%

Sterol Accumulation and Composition in Developing Zea mays L. Kernels

Davis

Poneleit²

1974

Plant Physiol.

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Kernels were collected from three maize (Zea inrays L.) inbreds from 10 days after pollination until kernel maturity. Sitosterol, campesterol, and stigmasterol were the major sterols at all stages of kernel development. Cholesterol was less than 1 % of the dry weight. The three major sterols accumulated during kernel development, but at a rate slower than dry weight. 10,14, 18, 22, 26, 30, 34, 38, 42, 50, and 60 DAP2 and

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“…In absolute terms, none of the individual sterols of the ester fraciton increased with increased 03 exposure, and only sitosterol increased in relative terms (Table IV) (4). The ratio of FS:BS has a much greater effect on membrane permeability than the composition of the FS fraction and, therefore, any significant decrease in FS:BS ratio results in cell injury.…”

Section: Grunwald and Endressmentioning

confidence: 99%

“…Tomlinson and Rich (10) have argued that during 03 exposure FS are converted to their glycosides and, thereby, increased membrane leakiness. This proposal is based on the observation that BS, such as glycosides and esters, are poor membrane stabilizers because they do not have a free C-3 hydroxyl group (4).…”

mentioning

confidence: 99%

Foliar Sterols in Soybeans Exposed to Chronic Levels of Ozone

Grünwald

Endress²

1985

Plant Physiol.

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ABSTRACr Soybeans (Glycine max) exposed to chronic levels of ozone showed a linear decrease in biomass with increasing concentration. The foliar free sterols increased while the steryl ester, and the steryl glycosides, a minor component, decreased with increasing pollutant concentration. Of the free sterols, stigmasterol showed the largest increase, followed by sitosterol; campesterol, however, decreased. All steryl esters decreased; sitosterol showed the largest decrease and campesterol the least.Ozone changes the function ofcellular membranes by inducing a loss in semipermeability (1, 2). This loss of semipermeability presumably results from 03-induced structural modifications of membranes, but the actual mechanism of oxidant injury is unclear (6). Following exposure to O3 (0.25 or 0.50 Ml l-l), Tomlinson and Rich (10,1) Sterol Analysis. Leaves were ground in acetone and extracted in a Soxhlet for 24 h. The acetone was removed under reduced pressure, the residue redissolved in methanol,,and the FS and SE extracted 3x with hexane. The SG fraction (alcohol phase) was acidified with Hc2SO4 to 0.5 N, refluxed for 12 h, cooled, neutralized and extracted 3x with hexane. The FS and SE were separated by column chromatography, using a 1.5 cm i.d. glass column packed with 30 g of silica gel (70-325 mesh) as a hexane slurry. The FS-SE fraction was applied in hexane and washed into the column with 50 ml hexane (discarded). Elution for SE was with 150 ml of 10% benzene in hexane followed by 700 ml of 40% benzene in hexane. The FS were eluted with 150 ml of benzene followed by 800 ml of chloroform. The

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Effects of Free Sterols, Steryl Ester, and Steryl Glycoside on Membrane Permeability

Cited by 152 publications

References 15 publications

Effects of n‐butanol and filipin on membrane permeability of developing wheat endosperms with different sterol phenotypes

Effects of n‐butanol and filipin on membrane permeability of developing wheat endosperms with different sterol phenotypes

Sterol Accumulation and Composition in Developing Zea mays L. Kernels

Foliar Sterols in Soybeans Exposed to Chronic Levels of Ozone

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