Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

Martins, Claudia Miranda; Báo, Sônia Nair; Dode, M. A. N.; Corrêa, G. A.; Rumpf, R.

doi:10.1016/j.theriogenology.2007.01.015

Cited by 75 publications

(68 citation statements)

References 36 publications

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“…The freeze-drying process of bull spermatozoa decreased the proportion of activated and fully-activated bovine oocytes after ICSI (Table 2). Adverse effect of drying on the ability of sperm cells to induce pronuclear formation has been previously reported after freeze-drying of bull [8,9] and porcine sperm [10] and after heat-drying of bull sperm [48]. Moreover, the adverse effect of freeze-drying was notable in the higher proportions of partially activated bovine oocytes after ICSI with FD versus control bull spermatozoa (Table 2).…”

Section: Discussionmentioning

confidence: 69%

“…Intracytoplasmic injection of freeze-dried spermatozoa has been resulted in successful production of live offspring in mouse [1,[4][5][6]32], rat [3,7,33] and rabbit [2], and blastocyst-stage embryos in cattle [8,9] and pig [10]. However, long-term storage of FD spermatozoa at room temperature has not yet been achieved in any mammalian species.…”

Section: Discussionmentioning

confidence: 99%

“…To date, preservation of FD spermatozoa for extended periods (≥1 year) was valid only at a refrigeration temperature in mouse [4, 5, and 6], rabbit [2] and rat [7]. On the other hand, blastocyst development after ICSI with FD sperm heads was reported in large domestic animals including cattle [8,9] and pig [10].…”

Section: Introductionmentioning

confidence: 99%

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The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

2009

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This study was designed to investigate the ability of freeze-dried (FD) bull spermatozoa to induce calcium oscillations in mouse oocytes and meiosis resumption in in-vitro-matured bovine oocytes after intracytoplasmic sperm injection (ICSI). Bull spermatozoa were freeze-dried and stored for one year at +25 ْ◌C, +4 ْ◌C, or -196 ْ◌C. In the first experiment, rehydrated sperm heads were microinseminated into hybrid mouse oocytes loaded with fluo-3/AM, and the kinetics of intracellular calcium concentration was monitored for 1 h. Repetitive increases of intracellular calcium concentration were recorded in the majority of injected oocytes, with exception of a few oocytes injected with FD sperm heads stored at +4 ْ◌C (11%) and +25 ْ◌C (8%) that exhibited a single increase or no response (non-oscillated). Proportion of oocytes oscillated with high frequency (≥10 spikes per hour) was significantly higher (P <0.05) in the non-dried control group (79%) than those in the FD groups (58, 55 and 54% for storage at -196, +4, and +25 ْ◌C, respectively). In the second experiment, control and FD spermatozoa were microinseminated into in-vitro-matured, denuded bovine oocytes. The oocytes were fixed and stained 12 h after ICSI. Significantly higher proportion of bovine oocytes injected with control spermatozoa (70%) resumed meiosis than those injected with +25, +4 and -196 ْ◌C-stored FD spermatozoa (53, 48 and 57%, respectively). The proportion of ICSI oocytes that developed to the pronuclear-stage (complete activation) was significantly higher in the control group (64%) than those in all the FD groups (34, 27, and 28% for storage at -196, +4, and +25 ْ◌C, respectively). Thus, the ability of bull spermatozoa to induce frequent intracellular calcium spikes in mouse oocytes was impaired by the process of freeze-drying, probably resulting in lower proportion of bovine oocytes that resumed meiosis and/or developed to the pronuclear-stage.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

2009

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“…Live sperm percentage was estimated using eosinnigrosin stained smears. Chromatin integrity was recorded using acridine orange (AO) staining technique [9,31].…”

Section: Semen Evaluationmentioning

confidence: 99%

Effect of Body Condition Score and PCR-RFLP Polymorphism of Prolactin Gene on Semen Characteristics of Buffalo Bulls (BubalusBubalis)

M.¹,

Hasanain

EL-Menoufy

et al. 2017

NIDOC-ASRT

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“…Medium1: 10 mmol/L Tris-HCl buffered supplemented with 50 mmol/L of each NaCl and EGTA [ethyleneglycol-bis (b-aminoethyl ether) All the media were designed according to Martins et al (2007) except medium 2 was designed according to Kaneko and Serikawa (2012).…”

Section: Freeze-drying Mediamentioning

confidence: 99%

The Effect of Freeze Drying Using Different Media and Storage Temperatures on Some Parameters of Buffalo Bull Spermatozoa

Shahba¹,

El-Sheshtawy

El-Azab

et al. 2016

NIDOC-ASRT

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HE PRESENT study was designed to display the effect of freeze-…..drying (lyophilization) technique using different freeze-drying media and storage temperatures on some parameters of buffalo bull spermatozoa. The semen samples were collected once weekly from five mature buffalo bulls maintained in Animal Reproduction Research Institute, Ministry of Agriculture, Al-Haram, Giza, Egypt. Samples were allocated into two portions; the first one was cryopreserved in Tris-Fructose-Egg yolk-Glycerol, while the second portion was immediately freeze-dried. These two portions were exposed to the same technical procedures. The freeze-dryer set was programmed at -55°C and 0.001 mbar pressure. The media tested were: EGTA solution, EDTA solution, TCM199 with Hanks salts enriched with 10% fetal calf serum (FCS) and TCM199 with Hanks salts enriched with 10% FCS and 0.2 M trehalose. The storage temperatures experienced were (4, -20, -80 ºC). The efficiency of each medium and storage temperature on some sperm parameters was explored. Our results revealed no motility either in raw or frozen-thawed spermatozoa after freeze-drying. In case of raw and frozen-thawed semen; with no respect to storage temperatures, the best freeze-dried sperm parameters were observed in TCM-Trehalose medium, while the best storage temperature was (-80°C) followed by (-20°C) with no respect to the type of freeze-drying media. From the present study, it was concluded that the freeze-drying medium containing trehalose could preserve efficiently components of buffalo bull spermatozoa, especially the acrosome, while the storage temperature (-80°C) and (-20°C) were the best for storage of freeze-dried buffalo bull spermatozoa.

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Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

Cited by 75 publications

References 36 publications

The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

Effect of Body Condition Score and PCR-RFLP Polymorphism of Prolactin Gene on Semen Characteristics of Buffalo Bulls (BubalusBubalis)

The Effect of Freeze Drying Using Different Media and Storage Temperatures on Some Parameters of Buffalo Bull Spermatozoa

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