Effects of freezing on the biomechanics of the intervertebral disc

Gleizes, V.; Viguier, É.; Feron, J.-M.; Canivet, S; Lavaste, F.

doi:10.1007/bf01653130

Cited by 44 publications

(20 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The effects of freezing on intervertebral discs has been the subject of several published studies, and the literature demonstrates there is no effect of freezing on stiffness, creep, hysteresis, and water content when such methods are used. [25][26][27][28][29] The linear association between motion segment height loss and water loss suggests that, at long time scales, creep of the intervertebral disc was associated with volume changes and not radial disc bulging. This is in direct agreement with magnetic resonance imaging measurements reporting relatively small amounts of radial bulging of the outer layer of the anulus fibrosus, indicating that the majority of volume loss was associated with loss in disc height.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, this study provides in vitro mechanical and water content data that may be used for comparison with the growing numbers of in vivo and organ culture studies using rat lumbar and caudal models. 6,7,10,12,13,[15][16][17]19,27,29 A quantitative association of relative contributions of disc bulging and volume loss would require a 3-dimensional multiphasic finite element model with intrinsic solid phase viscoelasticity, which is beyond the scope of this manuscript, although some similar models on human lumbar disc exist. 34…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Different Effects of Static Versus Cyclic Compressive Loading on Rat Intervertebral Disc Height and Water Loss In Vitro

Masuoka¹,

Michalek²,

MacLean³

et al. 2007

Spine

View full text Add to dashboard Cite

Study Design-In vitro biomechanical study on rat caudal motion segments to evaluate association between compressive loading and water content under static and cyclic conditions.Objective-To test hypotheses: 1) there is no difference in height loss and fluid (volume) loss of discs loaded in compression under cyclic (0.15-1.0 MPa) and static conditions with the same rootmean-square (RMS) magnitudes (0.575 MPa); and 2) after initial disc bulge, tissue water loss is directly proportional to height loss under static loading. Summary of Background Data-Disc degeneration affects water content, elastic and viscoelastic behaviors. There is limited understanding of the association between transient water loss and viscoelastic creep in a controlled in vitro environment where inferences may be made regarding mechanisms of viscoelasticity.Methods-A total of 126 caudal motion segments from 21 Wistar rats were tested in compression using 1 of 6 protocols: Static loading at 1.0 MPa for 9, 90, and 900 minutes, Cyclic loading at 0.15 to 1.0 MPa/1 Hz for 90 minutes, Mid-Static loading at 0.575 MPa for 90 minutes, and control. Water content was then measured in anulus and nucleus regions.Results-Percent water loss was significantly greater in nucleus than anulus regions, suggesting some water redistribution, with average values under 1 MPa static loading of 23.0% and 14.9% after 90 minutes and 26.9% and 17.6% after 900 minutes, respectively. Cyclic loading resulted in significantly greater height loss (0.506 ± 0.108 mm) than static loading with the same RMS value (0.402 ± 0.096 mm), but not significantly less than static loading at peak value (0.539 ± 0.122 mm). Significant and strong correlations were found between percent water loss and disc height loss, suggesting water was lost through volume decrease.Conclusion-Peak magnitude of cyclic compression and not RMS value was most important in determining height change and water loss, likely due to differences between disc creep and recovery rates. Water redistribution from nucleus to anulus occurred under loading consistent with an initial elastic compression (and associated disc bulge) followed by a reduction in disc volume over time.Address correspondence and reprint requests to James C. Iatridis, PhD, University of Vermont, 201 Perkins Building, 33 Colchester Ave, Burlington, VT 05405; E-mail: E-mail: james.iatridis@uvm.edu. The manuscript submitted does not contain information about medical device(s)/drug(s).No benefits in any form have been or will be received from a commercial party related directly or indirectly to the subject of this manuscript. NIH Public AccessAuthor Manuscript Spine (Phila Pa 1976 The intervertebral disc plays the essential biomechanical roles of supporting load and permitting motion in the spine. 1 The disc is a heterogeneous structure composed of a central nucleus pulposus surrounded by a highly organized fiber-reinforced anulus fibrosus. The nucleus pulposus is a hydrated gelatinous tissue composed of negatively charged glycosaminoglycans, coll...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Different Effects of Static Versus Cyclic Compressive Loading on Rat Intervertebral Disc Height and Water Loss In Vitro

Masuoka¹,

Michalek²,

MacLean³

et al. 2007

Spine

View full text Add to dashboard Cite

show abstract

“…All the specimens were allowed to thaw thoroughly overnight prior to use. Several groups have found no significant differences in mechanical properties of collagenous tissue after frozen storage 37383940…”

Section: Methodsmentioning

confidence: 99%

A biomechanical basis for tears of the human acetabular labrum

Smith

Masouros²,

Hill³

et al. 2008

Br J Sports Med

View full text Add to dashboard Cite

show abstract

“…All specimens were stored in physiological saline solution and kept frozen at −80°C until the day of testing. Since recent studies have shown that the collagen network of meniscal cartilage and the biomechanical response of intervertebral disc remain unchanged after frozen storage (Gleizes et al, 1998;Salai et al, 1997), the effects of freezing were assumed to be minimal in this study.…”

Section: Methodsmentioning

confidence: 99%

Anisotropy, inhomogeneity, and tension–compression nonlinearity of human glenohumeral cartilage in finite deformation

Huang

Stankiewicz

Ateshian

et al. 2005

Journal of Biomechanics

178

163

View full text Add to dashboard Cite

The tensile and compressive properties of human glenohumeral cartilage were determined by testing 120 rectangular strips in uniaxial tension and 70 cylindrical plugs in confined compression, obtained from five human glenohumeral joints. Specimens were harvested from five regions across the articular surface of the humeral head and two regions on the glenoid. Tensile strips were obtained along two orientations, parallel and perpendicular to the split-line directions. Two serial slices through the thickness, corresponding to the superficial and middle zones of the cartilage layers, were prepared from each tensile strip and each compressive plug. The equilibrium tensile modulus and compressive aggregate modulus of cartilage were determined from the uniaxial tensile and confined compression tests, respectively. Significant differences in the tensile moduli were found with depth and orientation relative to the local split line direction. Articular cartilage of the humeral head was significantly stiffer in tension than that of the glenoid. There were significant differences in the aggregate compressive moduli of articular cartilage between superficial and middle zones in the humeral head. Furthermore, tensile and compressive stressstrain responses exhibited nonlinearity under finite strain while the tensile modulus differed by up to two orders of magnitude from the compressive aggregate modulus at 0% strain, demonstrating a high degree of tension-compression nonlinearity. The complexity of the mechanical properties of human glenohumeral cartilage was exposed in this study, showing anisotropy, inhomogeneity, and tension-compression nonlinearity within the same joint. The observed differences in the tensile properties of human glenohumeral cartilage suggest that the glenoid may be more susceptible to cartilage degeneration than the humeral head.

show abstract

Effects of freezing on the biomechanics of the intervertebral disc

Cited by 44 publications

References 14 publications

Different Effects of Static Versus Cyclic Compressive Loading on Rat Intervertebral Disc Height and Water Loss In Vitro

Different Effects of Static Versus Cyclic Compressive Loading on Rat Intervertebral Disc Height and Water Loss In Vitro

A biomechanical basis for tears of the human acetabular labrum

Anisotropy, inhomogeneity, and tension–compression nonlinearity of human glenohumeral cartilage in finite deformation

Contact Info

Product

Resources

About