Effects of Freezing Stress on Photosynthetic Reactions in Cold Acclimated and Unhardened Plant Leaves

Krause, G. Heinrich; Klosson, Rupert J.

doi:10.1007/978-94-009-6813-4_27

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…5). In herbaceous higherplant species freezing affects photosynthesis and respiration either similarly, or respiration is distinctly less sensitive to a freeze-thaw treatment than photosynthetic CO2 uptake (Klosson and Krause 1981a;Thebud and Santarius 1981 ;Krause and Klosson 1983 ; see also Singh et al 1977). However, it cannot be excluded that in freeze-injured moss tissue photorespiration contributes to oxygen uptake of the cells (Rastorfer 1970).…”

Section: Discussionmentioning

confidence: 95%

Age-related differences in frost sensitivity of the photosynthetic apparatus of two Plagiomnium species

Rütten

Santarius

1992

Planta

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Shoots of two species of moss, Plagiomnium undulatum (Hedw.) Kop. and Plagiomnium affine (Funck) Kop., were subjected to freezing at various temperatures. After thawing, the activities of different photosynthetic reactions were determined in relation to the ages of the leaves. Analysis of the fast kinetics of chlorophyll-a fluorescence of individual leaves showed that young and old tissues were considerably less frost tolerant than mature ones. In principle, the pattern of freeze inactivation of photosynthetic reactions resembles that observed in higher plants. The decreases in the amplitude of Fv (variable fluorescence) and the ratio of Fv to Fm (maximum fluorescence) with increasing freezing stress reflect a progressive inactivation of photosystem II (PSII)-mediated electron transport, i.e. inhibition of photoreaction to photochemistry and-or electron donation to the photochemical reaction, and thus a decline in the potential photochemical efficiency of PSII. The insignificant change in the F0 (constant fluorescence) level during progressive decline of Fv indicates that the excitation-energy transfer between antenna pigments and from those to reaction centres of PSII was little impaired by lethal freezing stress. Sugar analyses of various stem sections showed that ontogenetic variation in the frost tolerance of leaves cannot be attributed to differences in the cellular levels of sucrose, glucose and fructose.

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Section: Discussionmentioning

confidence: 95%

Age-related differences in frost sensitivity of the photosynthetic apparatus of two Plagiomnium species

Rütten

Santarius

1992

Planta

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“…Moreover, the light-saturated rates of electron transport associated with PS II, PS I or the whole chain (Tab, 3) were not significantly affected after freezing at -7°C in darkness. Studies of cold-acclimated and unhardened leaves of spinach (Klosson and Krause 1981a,b) and several other herbaceous plants (Krause and Klosson 1983) have also shown that inhibition of COj fixation in initial stages of freezing damage occurred independently of thylakoid inactivation. Furthermore, ATP levels in the light and dark were still high when CO, fixation was strongly inhibited after thawing (Krause et al 1982), and uncoupling of photophosphoryiation was not observed.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of photosynthesis by freezing temperatures and high light levels in cold‐acclimated seedlings of Scots pine (Pinus sylvestris). – II. Effects on chlorophyll fluorescence at room temperature and 77 K.

Strand

Öquist

1985

Physiologia Plantarum

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Strand, M, and Oquist, G, 1985, Inhibition of photosynthesis by freezing temperatures and high liglit levels in cold-acclimated seedlings of Scots pine {Pinus sylvestris). -II. Effects on chlorophyll fluorescence at room temperature and 77 K. -Physiol, Plant. 65: 117-123, Shoots of cold-acclimated seedlings of Pinus sylvestris L, were exposed to a temperature of -7°C for 4 h, in darkness or at a photon flux density of 1 300 fimol m"~ s^B efore and after freezing, fluorescence kinetics of intact needles and isolated chioropiast membranes were measured at both room temperature and 77 K, Maximum and variable fluorescence yield of photosystem II both at room temperature and 77 K decreased strongly after freezing in light, whereas the initial fluorescence yieid was little affected. Quenching of maximum and variable fluorescence of photosystem I at 77 K also occurred. The results show that freezing in light damages photosystem Ii, thereby increasing the radiationless decay at the reaction centres of photosystem II, This is a typical symptom of photoinhibition of photosynthesis. Freezing in d.arkness did not significantiy reduce fluorescence yieid of photosystem il or photosystem I, Moreover, electron transport capacity was not significantly affected. We therefore suggest that the inhibition of the CO, assimilation in pine seedlings by freezing alone does not involve thyiakoid inactivation.Additional key words -Eiectron transport, freezing stress, photoinhibition, M. Strand (reprint requests) andG. Oquist,

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“…[7][8][9][10][11]. Obviously freezing injury to plant cells leading to inactivation of the photosynthetic apparatus is initiated at multiple sites, as a single primary site of frost damage cannot be recognized.…”

Section: Discussionmentioning

confidence: 99%

“…acclimation of the plant [7,8,9,11]. As a most sensitive indication of freezing injury of leaves, an inhibition of photosynthetic CO2 fixation was frequently observed, when the energy-conserving apparatus of the chloroplasts still appeared little affected [8,9,11].…”

Section: Introductionmentioning

confidence: 99%

“…As a most sensitive indication of freezing injury of leaves, an inhibition of photosynthetic CO2 fixation was frequently observed, when the energy-conserving apparatus of the chloroplasts still appeared little affected [8,9,11]. Transgression of a well-defined temperature limit of frost tolerance leads to a characteristic inhibition of photosynthetic electron transport.…”

Section: Introductionmentioning

confidence: 99%

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Freezing damage and frost tolerance of the photosynthetic apparatus studied with isolated mesophyll protoplasts of Valerianella locusta L.

Rumich-Bayer

Krause

1986

Photosynth Res

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Mesophyll protoplasts were isolated from unhardened and cold-acclimated leaves of Valerianella locusta L. and subjected to freeze-thaw treatment. To evaluate the extent and course of freezing injury, photosynthetic reactions of whole protoplasts and of free thylakoid membranes, liberated from protoplasts by osmotic lysis, were measured. In addition, the integrity of the protoplasts was determined by microscopy. The results reveal an increased frost tolerance of protoplasts isolated from acclimated leaves with respect to all parameters measured. CO2-dependent O2 evolution (representing net photosynthetic CO2 fixation of protoplasts) was the most freezing-sensitive reaction; its inhibition due to freeze-thaw treatment of protoplasts was neither correlated with disintegration of the plasma membrane, nor was it initiated by inactivation of the thylakoid membranes. The frost-induced decline of protoplast integrity was not closely correlated to thylakoid damage either. Freezing injury of the thylakoid membranes was manifested by inhibition of photosynthetic electron transport and photophosphorylation. Both photosystems were affected by freezing and thawing with strongest inhibition occurring in the water-oxidation system or at the oxidizing site of photosystem II. Photophosphorylation responded more sensitively to freezing stress than electron transport, although uncoupling (increased permeability of the thylakoid membranes to protons) was not a conspicuous effect. The data are discussed in relation to freezing injury in leaves and seem to indicate that frost damage in vivo is initiated at multiple sites.

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Effects of Freezing Stress on Photosynthetic Reactions in Cold Acclimated and Unhardened Plant Leaves

Cited by 9 publications

References 13 publications

Age-related differences in frost sensitivity of the photosynthetic apparatus of two Plagiomnium species

Age-related differences in frost sensitivity of the photosynthetic apparatus of two Plagiomnium species

Inhibition of photosynthesis by freezing temperatures and high light levels in cold‐acclimated seedlings of Scots pine (Pinus sylvestris). – II. Effects on chlorophyll fluorescence at room temperature and 77 K.

Freezing damage and frost tolerance of the photosynthetic apparatus studied with isolated mesophyll protoplasts of Valerianella locusta L.

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