Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function

Abstract

The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate copper metabolism in a way that affects redox homeostasis, thus contributing to progression of metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and decreased metallothionein level, while rendering the level of copper importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased renal copper level, and modulated renal copper metabolism as to preserve vital cellular function including mitochondrial energy production and antioxidative defense, at the expense of intracellular copper storage.

Development of a novel copper metabolism-related risk model to predict prognosis and tumor microenvironment of patients with stomach adenocarcinoma

“…Copper is the third most prevalent transition metal in humans and is required for the growth and reproduction of all eukaryotes ( Andreini et al, 2008 ). Copper homeostasis is related to cell proliferation, angiogenesis, and metastasis ( Fukai et al, 2018 ; ATP, 2023 ), abnormal copper metabolism can lead to various diseases such as Wilson’s disease, Menkes’ disease, and idiopathic copper toxicosis ( Shobha Devi et al, 2018 ; Tasić et al, 2022 ). Copper ions directly bind to tricarboxylic acid cycle components, triggering lipid-acylated protein aggregation and cell death.…”

Effect of Copper on the Function of Isolated Porcine Kidneys Stored Using Simple Hypothermia

“…Another relevant protein is CTR2 (copper transporter 2), which is located in vesicular structures within the cytoplasm. In contrast, Cu + and Cu 2+ ions are capable of interacting directly with DMT1 [ 9 , 10 ]. ATPases (ATP7A and ATP7B) regulate the concentration of copper ions in the cell and mediate their incorporation into enzyme proteins [ 6 ].…”