Effects of Fullerenes on Phospholipid Membranes: A Langmuir Monolayer Study

Wang, Zhining; Yang, Shihe

doi:10.1002/cphc.200900328

Cited by 49 publications

(47 citation statements)

References 44 publications

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“…Yang and Wang investigated two-component Langmuir monolayers of DPPC/fullerene by recording surface pressure/area and surface potential/area isotherms and by direct Brewster angle microscopy imaging. [66] It showed that the isotherms and elastic modulus versus π curves of the DPPC/fullerene were remarkably similar to the pure DPPC monolayers even at a molar fraction as high as Xc 60 = 0.3, suggesting little effect of fullerene. In our simulation, the ratio of the fullerene/DOPC is about 0.05, which is smaller than the experimental ratio.…”

Section: Resultsmentioning

confidence: 92%

Effect of Self-Assembly of Fullerene Nano-Particles on Lipid Membrane

Zhang

et al. 2013

PLoS ONE

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Carbon nanoparticles can penetrate the cell membrane and cause cytotoxicity. The diffusion feature and translocation free energy of fullerene through lipid membranes is well reported. However, the knowledge on self-assembly of fullerenes and resulting effects on lipid membrane is poorly addressed. In this work, the self-assembly of fullerene nanoparticles and the resulting influence on the dioleoylphosphtidylcholine (DOPC) model membrane were studied by using all-atom molecular dynamics simulations with explicit solvents. Our simulation results confirm that gathered small fullerene cluster can invade lipid membrane. Simulations show two pathways: 1) assembly process is completely finished before penetration; 2) assembly process coincides with penetration. Simulation results also demonstrate that in the membrane interior, fullerene clusters tend to stay at the position which is 1.0 nm away from the membrane center. In addition, the diverse microscopic stacking mode (i.e., equilateral triangle, tetrahedral pentahedral, trigonal bipyramid and octahedron) of these small fullerene clusters are well characterized. Thus our simulations provide a detailed high-resolution characterization of the microscopic structures of the small fullerene clusters. Further, we found the gathered small fullerene clusters have significant adverse disturbances to the local structure of the membrane, but no great influence on the global integrity of the lipid membrane, which suggests the prerequisite of high-content fullerene for cytotoxicity.

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Section: Resultsmentioning

confidence: 92%

Effect of Self-Assembly of Fullerene Nano-Particles on Lipid Membrane

Zhang

et al. 2013

PLoS ONE

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“…C s −1 describes the relationship between the surface pressure increase and the area per molecule decrease. A higher value of C s −1 is indicative of a less compressible monolayer [44,45].…”

Section: Parameters Studied From the Surface Pressure-area Langmuir Imentioning

confidence: 98%

Interplay of mycolic acids, antimycobacterial compounds and pulmonary surfactant membrane: A biophysical approach to disease

Pinheiro

Giner‐Casares

Lúcio

et al. 2013

Biochimica et Biophysica Acta (BBA) - Biomembranes

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This work focuses on the interaction of mycolic acids (MAs) and two antimycobacterial compounds (Rifabutin and N'-acetyl-Rifabutin) at the pulmonary membrane level to convey a biophysical perspective of their role in disease. For this purpose, accurate biophysical techniques (Langmuir isotherms, Brewster angle microscopy, and polarization-modulation infrared reflection spectroscopy) and lipid model systems were used to mimic biomembranes: MAs mimic bacterial lipids of the Mycobacterium tuberculosis (MTb) membrane, whereas Curosurf® was used as the human pulmonary surfactant (PS) membrane model. The results obtained show that high quantities of MAs are responsible for significant changes on PS biophysical properties. At the dynamic inspiratory surface tension, high amounts of MAs decrease the order of the lipid monolayer, which appears to be a concentration dependent effect. These results suggest that the amount of MAs might play a critical role in the initial access of the bacteria to their targets. Both molecules also interact with the PS monolayer at the dynamic inspiratory surface. However, in the presence of higher amounts of MAs, both compounds improve the phospholipid packing and, therefore, the order of the lipid surfactant monolayer. In summary, this work discloses the putative protective effects of antimycobacterial compounds against the MAs induced biophysical impairment of PS lipid monolayers. These protective effects are most of the times overlooked, but can constitute an additional therapeutic value in the treatment of pulmonary tuberculosis (Tb) and may provide significant insights for the design of new and more efficient anti-Tb drugs based on their behavior as membrane ordering agents.

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“…A higher value of C À1 s is indicative of a less compressible monolayer. [25,26] The A min value corresponds to the mean area occupied by one molecule of EPC in the surface layer just before collapse. It is indicative of both the molecular packing and the interactions between the monolayer components.…”

Section: Surface Pressure-molecular Area Isothermsmentioning

confidence: 99%

Evaluation of the Structure–Activity Relationship of Rifabutin and Analogs: A Drug–Membrane Study

et al. 2013

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This work focuses on the influence of rifabutin and two novel analogs, namely, N'-acetyl-rifabutin and N'-butanoyl-rifabutin, on the biophysical properties of lipid membranes. Monolayers and multilamellar vesicles composed of egg L-α-phosphatidylcholine:cholesterol in a molar ratio of 4:1 are chosen to mimic biological membranes. Several accurate biophysical techniques are used to establish a putative relationship between the chemical structure of the antimycobacterial compounds and their activity on the membranes. A combination of in situ experimental techniques, such as Langmuir isotherms, Brewster angle microscopy, polarization-modulated infrared reflection-absorption spectroscopy, and small-angle X-ray scattering, is used to assess the drug-membrane interaction. A relationship between the effect of a drug on the organization of the membranes and their chemical structure is found and may be useful in the development of new drugs with higher efficacy and fewer toxic effects.

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Effects of Fullerenes on Phospholipid Membranes: A Langmuir Monolayer Study

Cited by 49 publications

References 44 publications

Effect of Self-Assembly of Fullerene Nano-Particles on Lipid Membrane

Effect of Self-Assembly of Fullerene Nano-Particles on Lipid Membrane

Interplay of mycolic acids, antimycobacterial compounds and pulmonary surfactant membrane: A biophysical approach to disease

Evaluation of the Structure–Activity Relationship of Rifabutin and Analogs: A Drug–Membrane Study

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