Effects of Fungicide Euparen Multi (Tolylfluanid) on Development of Preimplantation Embryos in Mouse

Domaracký, M; Rehák, P; Legáth, Jaroslav; Koppel, Juraj

doi:10.2754/avb200776020209

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…Essential oils (EOs) can be extracted from fresh plant flowers or leaves and widely consumed as aroma additives in food, cosmetics, and pharmaceuticals. Thyme (Thymus vulgaris) is widely cultivated as herbal medicine, spice, and tea and is considered one of the well-known plants of Lamiaceae family and (Domaracký et al, 2007). Thyme possesses different beneficial effects, including bronchopulmonary disorders, sedative, antispasmodic, gastroenteric, anthelmintic, antimicrobial, carminative, diaphoretic, and antioxidant activities (Rustaiyan et al, 2000, Miura et al, 2002.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Thymus Vulgaris Oil Against CCL4-Mediated Hepatotoxicity, Oxidative Stress and Immunosuppression in Male Albino Rats

Elshopakey¹,

Risha²,

El‐Boshy³

et al. 2021

AAVS

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A variety of alternative medicine is aromatherapy that uses essential oils for treating and preventing disease. The current study was applied to investigate the possible protective effects of Thymus vulgaris essential oil (TEO) against carbon tetrachloride (CCl 4 ) toxicity. Forty-eight rats were equally allocated into six experimental groups and effectively treated for 4 weeks as follows: control group; the group treated with CCl 4 (1ml/ kg b.w) CCl 4 twice a week; the groups treated daily with low and high doses of TEO (2.5 and 5 mg/kg b.w) and the groups received CCl 4 twice a week and treated daily with TEO at the two tested doses. At the end of the treatment period, blood and tissue samples were collected for estimation of hematological, biochemical, antioxidant, immunological parameters, and histopathological assessment, and immunohistochemistry for cleave caspase and PCNA in hepatic tissue. CCl 4 significantly decreased all hematological parameters except neutrophils and toxic neutrophils counts were significantly increased. Moreover, CCl 4 increased the liver enzymes level and hepatic oxidative stress parameters. Meanwhile, the antioxidants, respiratory burst, phagocytic, serum lysozyme, and bactericidal activities were significantly suppressed in CCl 4intoxicated rats . Also, CCl 4 raised serum levels of TNF-α, IL-6, and diminished IL-10, IL-12. It also increased caspase 3 and PCNA. The treatment with T. vulgaris oil attenuated the toxic effect of CCl 4 in all hematological parameters. It also reduced the hepatotoxic effect of CCl 4 , enhanced the antioxidant system, and improved the innate immune parameters and inflammatory markers to their normal level. Based on histopathological analysis T. vulgaris oil ameliorates hepatocellular necrosis induced by CCl 4 . Immunohistochemical assessment depicted lowered immunoreactivity against cleaved caspase-3 expression, significantly in comparison to CCl 4 treated group. Meanwhile, significantly increases at PCNA Immunoreactivity which indicates hepatocellular regeneration in T. vulgaris oil. More pronounced improvement was noticed in the group received the high dose of TEO. Our results suggested that Thymus vulgaris essential oil has a potential antioxidant activity and many protective effects against liver toxicity induced by CCl 4 and this protection was dose-dependent.

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Section: Introductionmentioning

confidence: 99%

Protective Effects of Thymus Vulgaris Oil Against CCL4-Mediated Hepatotoxicity, Oxidative Stress and Immunosuppression in Male Albino Rats

Elshopakey¹,

Risha²,

El‐Boshy³

et al. 2021

AAVS

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“…Cloves show antiseptic, antibacterial, antifungal and antiviral properties . It is a popular remedy for dental disorders, respiratory disorders, headaches and sore throats in regular medicines of Australia and Asian countries . Besides its antimicrobial, antifungal and antiviral properties, clove displays cytotoxic, anesthetic and anti‐inflammatory properties .…”

Section: Introductionmentioning

confidence: 99%

Biomimetic synthesis of silver nanoparticles using Syzygium aromaticum (clove) extract: Catalytic and antimicrobial effects

Ajitha

Reddy

Lee

et al. 2019

Applied Organom Chemis

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In the current work, we followed a green chemistry route to prepare and characterize the silver nanoparticles (AgNPs) using Syzygium aromaticum (clove) extract at room temperature. Suitably, the clove extract acted as a reducing agent as well as a capping agent, and these reactions occurred rapidly. The formation of the AgNPs was confirmed by the observation of the distinct absorption peak at a wavelength of 418 nm using ultraviolet–visible (UV–Vis) spectroscopy, and a morphological study confirmed the uniform distribution of the optimally spherical nanoparticles. Fourier transform infrared spectroscopy (FTIR) results indicated the methoxy and allyl functional groups of eugenol of the clove extract to be responsible for the bioreduction of silver ions and for the stabilization of the resulting nanoparticles (flavonoids). We also found the AgNPs to be effective catalysts of the degradation of three pollutant organic dyes viz., 4‐nitrophenol, methylene blue and rhodamine B, in the presence of excess NaBH4. The antibacterial and antifungal activities of the bio‐synthesized AgNPs were also explored. Overall, the results suggested the potential use of clove extract as a resource for the synthesis of AgNPs having a broad range of possible commercial and biomedical applications.

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“…Multiple in vitro exposures of mouse thymocytes to pesticide were examined by Olgun et al (2004) . As regards tolylfluanid formulation (with 50% active agent), Domaracky et al (2007) reported cell death in mouse preimplantation embryos at relatively low doses. Nevertheless, the incidence of cell death has been mostly reported as necrosis.…”

Section: Discussionmentioning

confidence: 99%

Induction of SCEs and DNA fragmentation in bovine peripheral lymphocytes by in vitro exposure to tolylfluanid-based fungicide

2010

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The potential for genotoxic and cytotoxic effects of tolylfluanid-based fungicide (50% active agent) was evaluated using sister chromatid exchange (SCE) and proliferation indices (PI) in cultured bovine peripheral lymphocytes. For the detection of possible genetic damage, DNA fragmentation assay was also applied. Bovine lymphocytes cultured for 72 h were treated with the fungicide at the final concentrations of 1.75, 3.5, 8.75, and 17.5 μg/mL for the last 24 and 48 h of culture without S9 metabolic activation, and during the last 2 h of culture with S9 metabolic activation. In the SCE assays no evidence for genotoxic activity of the fungicide was found in treatments of 24 h without and 2 h with S9. After the 24 h exposure to tolylfluanid, a weak decrease in the PI was observed. With the prolonged exposure time (48 h), dose dependence in the increase of SCE frequencies was observed. Moreover, after 48 h exposure slight fragmentation of DNA at the concentrations of 3.5 and 8.75 μg/mL was demonstrated. SCE quantification is the most widely used approach for the assessment of genotoxic/cytogenetic effects of chemical compounds. Positive results in the assay at 48 h exposure indicated a potential of the fungicide to increase frequency of chromosomal damage (replication injuries) that is the confirmation of early effect of exposure.

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Effects of Fungicide Euparen Multi (Tolylfluanid) on Development of Preimplantation Embryos in Mouse

Cited by 6 publications

References 15 publications

Protective Effects of Thymus Vulgaris Oil Against CCL4-Mediated Hepatotoxicity, Oxidative Stress and Immunosuppression in Male Albino Rats

Protective Effects of Thymus Vulgaris Oil Against CCL4-Mediated Hepatotoxicity, Oxidative Stress and Immunosuppression in Male Albino Rats

Biomimetic synthesis of silver nanoparticles using Syzygium aromaticum (clove) extract: Catalytic and antimicrobial effects

Induction of SCEs and DNA fragmentation in bovine peripheral lymphocytes by in vitro exposure to tolylfluanid-based fungicide

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