Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro

Brown, D. M.; Groom, C. L.; Cvitanik, M.; Brown, Murray; Cooper, Jan L.; Arditti, Joseph

doi:10.1007/bf02318914

Cited by 20 publications

(7 citation statements)

References 13 publications

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“…It is common to observe some persistent yeast and/or bacterial strains that contaminate the explants of orchids cultivated in vitro, especially those derived from somatic tissues. In other orchid species, similar microorganisms were observed, such as in Habenaria radiata, in which 33% of explants from shoot apices were contaminated with bacteria (Mitsukuri et al 2009). Brown et al (1982, who tested different fungicides and antibiotics to prevent contamination in several orchid species, observed that only one flask from a treatment that combined a cocktail (benomyl, quintozene, penicillin G, amphotericin B and sodium omadine) resulted in uncontaminated seeds and zygotic embryos of Dendrobium specisum var.…”

Section: Conclusion Limitations and Future Perspectivesmentioning

confidence: 76%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Section: Conclusion Limitations and Future Perspectivesmentioning

confidence: 76%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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“…Besides these additives, increased frequency of seed germination in S. plicata has been reported earlier following enrichment of VW medium with other natural adjuncts such as extract of Amaranthus viridis and sugarcane separately or in combination (Roy and Biswas 2001). Enhancement in the rate of seed germination due to supplementation of nutrient medium with different types of additives has also been mentioned in several other orchid species (Arditti 1967, Goh 1975, Goh 1977, Charanasri and Wanichkul 1978, Uesato 1987, Brown et al 1982, Roy and Banerjee 2002.…”

Section: Discussionmentioning

confidence: 76%

Isolation of a White Flowered Mutant through Seed Culture in Spathoglottis plicata Blume

Roy

Biswas

2005

CYTOLOGIA

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Summary Spathoglottis plicata Blume, a terrestrial orchid having prolonged inflorescence with beautiful lilac (pinkish purple) coloured flowers, is commercially important. Treatment of its seeds with sodium azide induced strikingly attractive flower colour modification exploiting seed culture protocols. Vacin and Went (VW) (1949) medium stimulated seed germination and after supplementation with ripe banana extract yielded maximum germination frequency in all cases; it was followed with ripe tomato extract and yogurt water (semi-solid liquor from fermented milk) in untreated and post-treated seeds respectively. The magnitude, however, reduced in treated seeds. Germination initiated usually on the 20th day of inoculation, while addition of yogurt water induced earlier germination by 9 days in the treated and 7 days in untreated samples. Repeatedly subcultured 60 days old plantlets were gradually acclimatized growing inside a hardening chamber and subsequently in a glass house. A treated plant with deep green leaves showed conspicuous variation from the mother strain by its charming milk-white flower colour and green floral bracts in contrast to lilac coloured flower and floral bracts in the latter. VM 2 progeny retained the mutant phenotype and genetic uniqueness as indicated by specific PAGE protein banding profile. The mutant having completely different flower colour modification with milky whiteness possesses special floricultural significance and may, therefore, be treated as a new horticultural variety.

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“…Surface sterilization of the seeds or tissues can prevent initial contamination, but microorganisms often find their way into cultures at a later date. Incorporation of benomyl and another fungicides and bactericides in several combinations, can prevent or inhibit contamination of C. aurantiaca and Stanhopea occulata seedlings in vitro and Phalaenopsis flower stalk node cultures without adversely affecting the plants (Brown et al 1982). Bayman et al (2002) demonstrated that benomyl-treated plants of the orchid Lephantes rupestris had significantly lower mortality than controls (treated with water), and significantly fewer fungi in leaves that control plants.…”

Section: Discussionmentioning

confidence: 99%

Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase

Franco

Guevara

Mesa

et al. 2007

RBT

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Abstract:Cattleya trianae is an endemic species from the tropical rainforest in the Colombian Andes. Its survival is currently threatened due to habitat loss and commercial overexploitation. This study evaluates ten substrates, some organic (pine bark, coconut fiber and wood shavings), some inert icopor (polystyrene foam), vegetable coal and their combinations, and the effects these have on morphometric and phenotypic traits in the hardening phase of 250 plants of C. trianae cultivated in vitro. Recorded data include percent survival, length of longest leaf, biomass (wet weight) and number of roots and leaves at the beginning and at the end of the experiment. After the hardening phase, the plants were taken to a greenhouse and later to the natural environment. Coconut fiber alone or mixed in equal parts with pine bark and coal was the most efficient substrate when percent survival (80±SE=0.3742), biomass, and leaf length were evaluated. Hardened plants displayed qualitative characteristics such as vigor, hardness and waxy texture, strength of green coloration in the leaves, and velamen formation. Under greenhouse conditions, plants grew better with filtered light, relative humidity bordering on 80 %, permanent aeration, misting with water, and an average temperature of 25±2 °C. Invigorated plants were firmly anchored on their host trees. Rev. Biol. Trop. 55 (2): 681-691. Epub 2007 June, 29.

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Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro

Cited by 20 publications

References 13 publications

Tissue disinfection for preparation of Dendrobium in vitro culture

Tissue disinfection for preparation of Dendrobium in vitro culture

Isolation of a White Flowered Mutant through Seed Culture in Spathoglottis plicata Blume

Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase

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