Effects of Fusu mixture (Wen-Shen-Qian-Yang Method) on sepsis-induced acute respiratory distress syndrome

Zhang, Li; Long, Kunlan; Wang, Chunxia; Zhang, Xuemei; Yang, Hongjing; Chen, Jun; Li, Xue; Gao, Peiyang; Zhang, Song

doi:10.1097/md.0000000000021066

“…Traditional Chinese medicine (TCM) has shown suppressive effects on LPS-induced LPS-endothelial inflammation, including oxidative stress, cell apoptosis, microcirculatory disturbances and organ injury [10]. This protection on endothelium was validated in clinical practice on treatment of sepsis-induced acute respiratory distress syndrome [11]. Moreover, animal model showed that TCM decoction can ameliorate sepsis-induced acute lung injury, and the mechanism may be associated with decreased production of pro-inflammatory mediator by TCM decoction in LPS-pretreated HUVEC [12].…”

Section: Introductionmentioning

confidence: 99%

Salidroside protects endothelial cells against LPS-induced inflammatory injury by inhibiting NLRP3 and enhancing autophagy

You

¹

,

Zhang

²

,

Geng

³

et al. 2021

BMC Complement Med Ther

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Background Salidroside (SAL) is a bioactive compound extracted from Rhodiola rosea with various biological properties. This study was designed to explore the functions of SAL on the endothelial damage induced by lipopolysaccharide (LPS) and its related mechanisms. Methods Human umbilical vein endothelial cells (HUVECs) were pretreated with SAL (0, 10, 25, 50, 100 μM), and then incubated with LPS (10 μg/mL). Cell viability was evaluated by MTT assay, cell injury by lactate dehydrogenase (LDH) release, and inflammatory cytokines release by ELISA assay. Oxidative stress was evaluated by malondialdehyde (MDA) and superoxide dismutase (SOD) in cell lysate. Apoptosis was detected by flow cytometry and caspase-3 activity. Western blot were performed to determine expression levels of autophagy and NOD-like receptor protein 3 (NLRP3) related proteins. Results SAL at 50 μM concentration showed no toxicity on HUVECs, but attenuated LPS-induced injury, as evidenced by increased cell viability, reduction in LDH level and inflammatory cytokines in culture media. SAL also reduced MDA level and increased SOD activity in HUVECs, and inhibited apoptosis rate and caspase-3 activity. (P < 0.05). Moreover, LPS enhanced HUVECs autophagy, and SAL pretreatment further enhanced autophagy, with increased Beclin-1 protein and decreased P62 protein. SAL also attenuated LPS-induced activation of NLRP3 inflammasome, reduced the protein expression of NLRP3-related proteins, including ASC and caspase-1. Autophagy inhibition by 3-MA markedly reversed SAL-modulated changes in cell viability and NLRP3 expression in LPS-stimulated HUVECs. Conclusion SAL protects endothelial cells against LPS-induced injury through inhibition of NLRP3 pathways and enhancing autophagy.

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“…The main components in the decoction included ginseng, honeysuckle, rhubarb, dandelion, wild chrysanthemum, and so on. The existing pharmacology confirmed that ginseng has the effect of regulating immune function and the neuroendocrine system (19); honeysuckle is a good medicine for clearing away heat and toxic substances, which can remove endotoxin and improve the body's immunity (8); wild chrysanthemum and dandelion can sterilize, having the effect of detoxification and diuresis (11); rhubarb helps to repair intestinal microecology, inhibit bacterial reproduction, and improve gastrointestinal function, thereby reducing inflammation (13). A study (14) used acupuncture to stimulate specific acupoints, improving the immunity of sepsis patients and improving clinical treatment outcomes.…”

Section: Discussionmentioning

confidence: 99%

Effect of traditional Chinese medicine on serum inflammation and efficacy in patients with sepsis: a systematic review and meta-analysis

Wen¹,

Feng²,

Chen³

et al. 2021

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Background: Sepsis is a systemic infectious inflammatory response syndrome, which can release a large number of inflammatory mediators in serum, resulting in organ dysfunction. This study conducted a systematic review and meta-analysis of the reports of traditional Chinese medicine (TCM) in the treatment of sepsis in recent years, providing evidence for clinical diagnosis and treatment.Methods: The databases of PubMed, Chinese Medical Literature (CBM), China National Knowledge Infrastructure (CNKI), Medline, and Web of Science were searched for clinical randomized controlled trials (RCTs) related to "Chinese traditional medicine" and "Sepsis" published from January 2015 to August 2021.After screening qualified articles, RevMan 5.4 software was used to evaluate the bias of included studies and perform meta-analysis.Results: Finally, 8 articles were retrieved and screened, and a total of 862 patients entered the study. The results of meta-analysis showed that serum interleukin-6 (IL-6) levels were lower in sepsis patients after

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“…The vascular endothelial cells (VECs) play a role in regulating vascular permeability, vascular tone, and blood flow [ 6 ]. Its mechanisms include forming new blood vessels by proliferating, migrating, differentiating, and maintaining microvascular integrity and blood flow via anti-inflammatory, antithrombotic, and vasodilatory activities [ 7 , 8 ]. Nonetheless, mature VECs are limited by such defects as low activity, slow growth, and renewal.…”

Section: Introductionmentioning

confidence: 99%

Association of BIRC5 Gene Polymorphism with the Collateral Circulation and Severity of Large Artery Atherosclerotic Stroke

Huang

¹

,

Li

²

,

Zhao

³

et al. 2022

International Journal of Clinical Practice

1

0

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Objectives. The collateral circulation near the cerebral artery occlusion can contribute to the relief of the symptoms and signs of stroke. Genetic factors play a decisive role in the difference in collateral circulation. Survivin, encoded by the baculoviral inhibitor of apoptosis (IAP) repeat-containing 5 gene (BIRC5), plays an important role in maintaining long-term endothelial integrity and homeostasis and as an angiogenic factor in the treatment of vascular diseases. We hypothesized that genetic variations in the BIRC5 gene may contribute to severity by influencing the collateral circulation. This study aimed at examining how the polymorphism of the BIRC5 gene correlated with the collateral circulation and severity of large artery atherosclerotic stroke. Methods. This study enrolled 428 patients with large artery atherosclerotic stroke. There are no statistical differences in age, sex, social behavior, such as smoking and drinking, between the groups classified by the collateral circulation and by the severity of stroke ( P > 0.01 ). Direct sequencing was performed for the genotyping of single nucleotide polymorphism (SNP) of BIRC5 (rs2071214). The enrolled patients were divided into several subgroups based on the collateral flow grading system from the American Society of Interventional and Therapeutic Neuroradiology/Society of Interventional Radiology (ASITN/SIR), the results of the National Institutes of Health Stroke Survey (NIHSS) (6 as a threshold), and the score of the modified Rankin scale (mRS) (for the prediction of prognosis, 2 as a threshold). Differences among subgroups were identified through logistic regression. Results. The analysis of collateral circulation revealed the significant correlation of SNP of rs2071214 with the development of poor collateral circulation of large artery atherosclerotic stroke in the additive model (GG vs. AA, odds ratio (OR) = 3.592, 95% confidence interval (CI) = 1.410–9.150, and P = 0.007 ) and the recessive model (GG vs. AA/GA, OR = 3.313, 95% CI = 1.420–7.727, and P = 0.006 ). The analysis of stroke severity exposed the significant role of the SNP of rs2071214 in increasing stroke severity in the dominant model (GA/GG vs. AA, OR = 1.658, 95% CI = 1.017–2.703, and P = 0.043 ) and the additive model (GA vs. AA, OR = 1.717, 95% CI = 1.021–2.888, and P = 0.042 ). However, the analysis of the short-term outcome indicated that three genetic models were not associated with short-term outcomes in the additive model (GA vs. AA, P = 0.815 , GG vs. AA, and P = 0.336 ), the dominant model (GA/GG vs. AA and P = 0.589 ), and the recessive model (GG vs. AA/GA and P = 0.342 ). Conclusion. Our findings identified the SNP of rs2071214 of the BIRC5 gene as a risk factor for the poor compensatory ability of collateral circulation and a predictor of stroke severity in large artery atherosclerotic stroke, which suggested that the SNP of rs2071214 can serve as an innovative therapeutic target for patients with acute ischemic stroke.

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Effects of Fusu mixture (Wen-Shen-Qian-Yang Method) on sepsis-induced acute respiratory distress syndrome

Abstract: Supplemental Digital Content is available in the text

Cited by 4 publications

References 24 publications

Salidroside protects endothelial cells against LPS-induced inflammatory injury by inhibiting NLRP3 and enhancing autophagy

Salidroside protects endothelial cells against LPS-induced inflammatory injury by inhibiting NLRP3 and enhancing autophagy

Effect of traditional Chinese medicine on serum inflammation and efficacy in patients with sepsis: a systematic review and meta-analysis

Association of BIRC5 Gene Polymorphism with the Collateral Circulation and Severity of Large Artery Atherosclerotic Stroke

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