2003
DOI: 10.1038/sj.bjp.0705289
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Effects of G‐protein‐specific antibodies and Gβγ subunits on the muscarinic receptor‐operated cation current in guinea‐pig ileal smooth muscle cells

Abstract: 1 The effects on the whole-cell carbachol-induced muscarinic cationic current (mIcat) of antibodies against the a-subunits of various G proteins, as well as the effect of a Gbg subunit, were studied in single guinea-pig ileal smooth muscle cells voltage-clamped at À50 mV. Ionized intracellular calcium concentration, [Ca 2+ ] i , was clamped at 100 nM using a 1,2-bis(2-aminophenoxyl-ethane-N,N,N 0 ,N 0 -tetraacetic acid)/Ca 2+ mixture. 2 Application of ascending concentrations of carbachol (1 -300 mM) acti… Show more

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Cited by 48 publications
(75 citation statements)
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“…This is similar to mI CAT for which internal Ca 2ϩ is also required (36). Furthermore, mI CAT is blocked by PTX treatment (37,38), an effect ascribed to G o protein involvement (39,40). We therefore hypothesized that the failure of carbachol to reliably induce I TRPC4 could be due to the lack of a G o -coupled mAChR in HEK293 cells.…”
Section: Pip 2 Inhibits MI Cat Activation In An F-actin-dependent Manmentioning
confidence: 97%
“…This is similar to mI CAT for which internal Ca 2ϩ is also required (36). Furthermore, mI CAT is blocked by PTX treatment (37,38), an effect ascribed to G o protein involvement (39,40). We therefore hypothesized that the failure of carbachol to reliably induce I TRPC4 could be due to the lack of a G o -coupled mAChR in HEK293 cells.…”
Section: Pip 2 Inhibits MI Cat Activation In An F-actin-dependent Manmentioning
confidence: 97%
“…In these cell types, the I cat response is abolished by pertussis toxin (PTX) which selectively uncouples G i / G o proteins from the associated receptors (2, 7 -10). With the use of antibodies, the G protein involved in the signal transduction was identified as G o protein (11,12). Based on these results and the general thought that M2 receptors preferencially couple PTX-sensitive G proteins (8), the M2 subtype is supposed to mediate cationic channel opening through G o proteins.…”
Section: Introductionmentioning
confidence: 98%
“…Similar tests were made for Ga q / Ga 11 -antibody, where cells were dialyzed with the antibody (1:100 v / v) for 18 -20 min in order to allow it to diffuse adequately into the cell (12). With such antibody treatment, carbachol (100 m M) evoked no noticeable I K-Ca , whereas subsequent caffeine did evoke sizable I K-Ca (3.68 ± 0.64 nA, n = 6) (Fig.…”
Section: Effectiveness Of U73122 and Ga Q /Ga 11 -Antibody In Inhibitmentioning
confidence: 99%
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