Effects of Galium aparine extract on the cell viability, cell cycle and cell death in breast cancer cell lines

Atmaca, Harika; Bozkurt, Emir; Cittan, Mustafa; Tepe, Hafize Dilek

doi:10.1016/j.jep.2016.04.007

Cited by 21 publications

(22 citation statements)

References 13 publications

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“…Z grupy glikozydów irydoidowych występują: asperulozyd, kwas asperulozydowy i deacetyloasperulozydowy [1,5]. Dotąd wykryte kwasy fenolowe to: kwas wanilinowy, 3,4-dihydroksybenzoesowy, p-hydroksycynamonowy, galusowy, 4-hydroksytruksylowy [6], p-kumarowy, ferulowy [1, 2, 7], kawowy, p-hydroksybenzoesowy, salicylowy i chlorogenowy [1,2,8]. Do frakcji czynnych należą flawonoidy pochodne: luteoliny, chryzoeriolu, apigeniny i kwercetyny oraz ich glikozydowe pochodne [8,9].…”

Section: Związki Chemiczneunclassified

“…Dotąd wykryte kwasy fenolowe to: kwas wanilinowy, 3,4-dihydroksybenzoesowy, p-hydroksycynamonowy, galusowy, 4-hydroksytruksylowy [6], p-kumarowy, ferulowy [1, 2, 7], kawowy, p-hydroksybenzoesowy, salicylowy i chlorogenowy [1,2,8]. Do frakcji czynnych należą flawonoidy pochodne: luteoliny, chryzoeriolu, apigeniny i kwercetyny oraz ich glikozydowe pochodne [8,9]. Ponadto, w G. aparine występują alkaloidy izochinolinowe (protopina), indolowe (harmina) oraz typu chinazoliny [1, 2], kwasy tłuszczowe, terpeny [10], fitosterole (m.in.…”

Section: Związki Chemiczneunclassified

“…Ponadto, w G. aparine występują alkaloidy izochinolinowe (protopina), indolowe (harmina) oraz typu chinazoliny [1, 2], kwasy tłuszczowe, terpeny [10], fitosterole (m.in. β-sitosterol, daukosterol), ftalan dibutylu [10,11], kumaryna i eskuletyna [8]. Przebadany został skład olejku otrzymanego z nadziemnych części G. aparine rosnącej w Turcji.…”

Section: Związki Chemiczneunclassified

“…Istnieją doniesienia potwierdzające aktywność przeciwnowotworową przytulii czepnej. Wyciąg metanolowy wykazywał właściwości antyproliferacyjne i apoptotyczne w komórkach ludzkiego raka piersi (MCF-7 i MDA-MB-231), natomiast wobec komórek nabłonkowych piersi (MCF-10A) nie był cytotoksyczny [8]. Działanie antyproliferacyjne frakcji eterowej 60% wyciągu etanolowego z ziela G. aparine zostało ocenione na linii białaczkowej (K562).…”

Section: Działanie Biologiczne Badania In Vitro I In Vivounclassified

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Analysis of essential oil of Galium aparine.

Antoniak¹,

Dudek-Makuch²,

Bylka³

2019

Farm Pol.

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Analysis of essential oil of Galium aparine • Galium aparine L. (clivers, goosegrass) (Rubiaceae) is used in folk medicine as diuretic and medicinal plant alleviating different dermatoses as psoriasis, seborrheic dermatitis, eczema, and improves the immune system. So far, scientific studies have shown as a main constituents: iridoids (mainly asperuloside), flavonoids (luteolin, chrysoeriol, apigenin and quercetin derivatives), numerous of phenolic acids and phytosterols. Previous studies confirmed antimicrobial, antioxidant and cytotoxic activity of clivers. The aim of this work was to investigate the chemical profile of essential oil isolated from G. aparine collected in Poland. The essential oil was extracted from the herb of G. aparine by hydrodistillation in Clevenger-type apparatus and the volatile constituents were identified by gas chromatography coupled with mass spectrometry (GC-MS). 0.1 ml of essential oil (3.33 ml/kg) was obtained from the of G. aparine herb, in which 74 volatile ingredients were identified (100%). According to GC/MS analysis 9-diazo-9H-fluorene (31.93%), palmitic acid and dihomo-γ-linolenic acid (8.89%) were as major constituents. 9-diazo-9H-fluorene is very rare in the plant world, previously identified only in Bidens pilosa. Terpene compounds represented 22.31% of essential oil, including 15 sesquiterpenes (18.23%), 6 monoterpenes (2.00%) and 1 diterpene (3.08%). The other active ingredients of the oil include: phytol, β-ionone, anethole, spatulenol, α-cadinol, phenylethyl alcohol, eugenol, methyleugenol, α-carvacrol, α-caryophyllene, α-terpineol, isoaromadendren epoxide, carvone, linalool, santalol, β-farnesene and eucalyptole. The ingredients of the essential oil are multi-directional activity: anti-inflammatory, antimicrobial, anti-cancer, antiproliferative, effect on angiogenesis, and also have occlusive properties. Quantify and a number of ingredients of the essential oil have proven that it may be one of the bioactive important fractions of G. aparine.

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Section: Związki Chemiczneunclassified

Section: Działanie Biologiczne Badania In Vitro I In Vivounclassified

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Analysis of essential oil of Galium aparine.

Antoniak¹,

Dudek-Makuch²,

Bylka³

2019

Farm Pol.

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“…In our investigation, extracts of Gallium verum (Lady's Bedstraw) were used as test model. The plant, currently involved in traditional folk medicine, can be easily recognized by its distinct sunny yellow color and tiny little flowers [22][23][24]. As a remedy, Lady's Bedstraw is used for skin problems (burns, psoriasis), kidney and bladder stones and epilepsy (due to its sedative effect).…”

Section: Introductionmentioning

confidence: 99%

Image Analysis Approaches to Improve the Thin Layer Chromatography – Chemometric-Based Investigations of Natural Extracts

Simion¹,

Cobzac²

2017

Studia UBB Chemia

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The combination of high-performance thin-layer chromatography (HPTLC) with image analysis (IA) and chemometrics becomes an attractive tool for natural extracts investigations. The large variability of these samples requires powerful image acquisition devices, multivariate image processing techniques and advanced chemometric methods to facilitate the interpretation of the chromatographic data. In the current study, two image acquisition devices and different image processing procedures were investigated using the HPTLC chromatograms of hydroalcoholic extracts of Gallium verum. Different sets of chromatographic data were generated for both UV chromatograms (obtained at 254 nm and at 366 nm) using images acquired with a digital camera and an UV-Vis TLC scanner. In all cases the Principal Component Analysis (PCA) technique was used in order to extract the information from chromatographic profiles. Variables of gray and pure colour red, green and blue intensities of pixels from start to front were used as input data in all cases. The results obtained by PCA investigations of HPTLC data from UV chromatograms at 254 nm and 366 nm respectively, provided complementary information related to the characteristics of the investigated extracts. Moreover, important steps as appropriate color scale selection and image processing/analysis procedures were pointed out based on the obtained results.

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The effects of methanolic extract of the aerial parts of Galium verum on HT29 and AGO cell lines

Pashapour

Heshmati

Mousavi

et al. 2021

Nucleus

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The study reports the effects of methanol extract of Gallium verum on the cell proliferation of HT-29 (colon cancer) and AGO (fibroblast) cell lines. The cell lines were exposed to the G. verum extracts at the concentrations 12.5-400 μg/mL for a period of 72 h. Cell viability was evaluated by MTT assay. Apoptosis and cycle distribution were evaluated using flow cytometry, and RT-PCR was utilized for evaluating gene expression of the apoptotic cells. The results showed that the highest concentration (400 μg/mL) induced a significant decrease of cell viability in both HT29 and AGO cell lines. The percentage of apoptotic cells in the experimental group increased significantly when compared to the control group. The cell cycle was affected by an increased distribution of cells at G0/G1 phase and decreased occurrence of cells at S phase. The generation of reactive oxygen species (ROS) and the BAX/BCL2 ratio was found to be higher in the experimental group than that of the control group, respectively. Gallium verum at 400 μg/mL was found to decrease cell viability, induce apoptosis, increase BAX/BCL2 ratio and ROS production, and arresting cells at the G0/G1 phase.

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Effects of Galium aparine extract on the cell viability, cell cycle and cell death in breast cancer cell lines

Cited by 21 publications

References 13 publications

Analysis of essential oil of Galium aparine.

Analysis of essential oil of Galium aparine.

Image Analysis Approaches to Improve the Thin Layer Chromatography – Chemometric-Based Investigations of Natural Extracts

The effects of methanolic extract of the aerial parts of Galium verum on HT29 and AGO cell lines

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