Effects of gallic acid on rat testopathy following morphine administration: an experimental study

Abstract

Introduction: Morphine (MOR) as a psychoactive agent in the opium family causes free radicals accumulation which leads to failure in spermatogenesis. Gallic acid (GA), a polyphenolic acid, is found in various plants with antioxidant, anti-fungal, anti-viral, and anti-allergic activities. The purpose of this study was to evaluate the effects of GA against MOR-induced damage to the reproductive parameter of rats. Methods: Sixty-four male Wistar rats (8 weeks, 220-250 g) were categorized into 8 groups by random (n=8/each); normal control and MOR control groups; GA groups (5, 10, 20 mg/kg) and MOR + GA groups (5, 10, 20 mg/kg). Treatments were administered intraperitoneally (i.p), daily for 4 weeks. The sperm parameters, spermatogenesis index (SI), total antioxidant capacity, testosterone level, and seminiferous tube diameter (STD) were assessed. Results: All sperm parameters reduced significantly in the MOR control group than to the normal control group (P < 0.01). All parameters were significantly improved in GA and GA + MOR treatment groups compared to the MOR control group (P < 0.01). Conclusion: MOR caused a detrimental effect on male reproductive parameters. Also, no significant modifications were observed in all doses of GA treatments in comparison with the normal control group. GA compensates the toxic effect of MOR on reproductive parameters. Hence, GA administration is beneficial in MOR users.

Evaluation of supplementation of cryopreservation medium with gallic acid as an antioxidant in quality of post‐thaw human spermatozoa

“…Another study showed that utilizing gallic acid in male rats treated with lead acetate reduces the level of MDA in the testis tissue (Bello & Idris, 2018). Gallic acid was found to improve sperm count, motility, viability, morphology, serum testosterone, and total antioxidant levels in male rats given morphine (Jalili et al, 2019).…”

“…# p < 0.05 when compared to the cryopreserved control and the 100 μg/ml gallic acid group. The data is presented as the mean ± SEM sperm count, motility, viability, morphology, serum testosterone, and total antioxidant levels in male rats given morphine (Jalili et al, 2019).…”

“…Some studies have demonstrated that GA improves the quality of cryopreserved sperm in animals such as rams and goats. However, the efficient dose of gallic acid that reduces the harmful effects of cryopreservation on sperm is different in these studies (Ozturk et al, 2020; Gungor et al, 2019; Jalili et al, 2019).…”

“…However, the efficient dose of gallic acid that reduces the harmful effects of cryopreservation on sperm is different in these studies (Ozturk et al, 2020;Gungor et al, 2019;Jalili et al, 2019).…”