Effects of gamma irradiation and 15 days of subsequent ex vivo storage on the cytosolic red blood cell proteome analyzed by 2D‐DIGE and Orbitrap MS

Abstract

Gamma irradiation and subsequent ex vivo storage according to the Council of Europe guidelines were found to affect RBC protein structures. The validated marker proteins can serve as a basis for the development of a screening assay to monitor the quality of irradiated RBC concentrates during ex vivo storage.

Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review

“…Irradiation of blood is recommended to prevent TA-GVHD and to reduce the side effects of transfusions, transplants, and cancer therapies (Weinmann et al, 2000;Agarwal et al, 2005;Walpurgis et al, 2013;Santacruz-Gomez et al, 2014;Tabatabaei et al, 2016;Acosta-Elías et al, 2017). The blood can be irradiated before, during, and after storage, depending on the patient's necessities, but a common characteristic of irradiated blood is that RBCs are prone to hemolysis and, therefore, they are more fragile in circulation.…”

“…Studies with AFM have shown dose-dependent damage on mice RBCs after gamma irradiation, with the appearance of nanopores, crater-like vesicles, and changes in membrane roughness (Walpurgis et al, 2013;AlZahrani and Al-Sewaidan, 2017;Taqi et al, 2019). Here, AFM renders invaluable information at nanoscale dominions on RBCs under irradiation stress.…”

The Role of Translational Regulation in Survival after Radiation Damage; an Opportunity for Proteomics Analysis

“…The removal of T-lymphocytes helps prevent graft-versus-host reactions during transfusion. To study the long term effect of high dose IR, red blood cells (RBCs) were irradiated with 30 Gy, and proteins were differentially dye-labeled and then separated by 2D gel electrophoresis [ 74 ]. Protein spots that showed differences between irradiated and controls were identified by in-gel digestion followed by mass spectrometry (LC-MS/MS).…”