Effects of gamma irradiation dose-rate on sterile male Aedesaegypti

Ernawan, Beni; Tambunan, Usman Sumo Friend; Sugoro, Irawan; Sasmita, Hadian Iman

doi:10.1063/1.4985401

Cited by 19 publications

(15 citation statements)

References 25 publications

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“…A negative relationship between male competitiveness and irradiation dose has been observed in several times in studies on mosquitoes, including Aedes spp. [ 12 , 21 , 32 , 36 , 38 , 39 , 40 ]; however, irradiated males have been shown to be competitive after release in several studies [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Sexual Competitiveness and Induced Egg Sterility by Aedes aegypti and Aedes albopictus Gamma-Irradiated Males: A Laboratory and Field Study in Mexico

Bond

Aguirre-Ibáñez

Osorio

et al. 2021

Insects

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The sterile insect technique may prove useful for the suppression of mosquito vectors of medical importance in regions where arboviruses pose a serious public health threat. In the present study, we examined the effects of sterilizing irradiation doses across different ratios of fertile:irradiated males on the mating competitiveness of Ae. aegypti and Ae. albopictus under laboratory and field-cage conditions. For both species, the percentage of females inseminated and the number of eggs laid over two gonotrophic cycles varied significantly in mating treatments involving 1:1, 1:5, and 1:10 fertile:irradiated males compared to controls of entirely fertile or entirely irradiated males but was not generally affected by the irradiation dose. Egg hatching was negatively affected in females exposed to increasing proportions of irradiated males in both laboratory and field cages. Male competitiveness (Fried’s index) values varied from 0.19 to 0.58 in the laboratory and were between 0.09 and 1.0 in field cages, depending on th species. Competitiveness values were negatively affected by th eirradiation dose in both species under field-cage conditions, whereas in the laboratory, Ae. albopictus was sensitive to the dose but Ae. aegypti was not. In general, male competitiveness was similar across all mating regimes. Most importantly, induced egg sterility was positively correlated with the proportion of irradiated males present in the mating treatments, reaching a maximum of 88% under field-cage conditions for both Ae. aegypti and Ae. albopictus males treated with 50 and 40 Gy irradiation, respectively. These results indicate that sterile males produced at our facility are suitable and competitive enough for field pilot SIT projects and provide guidance to decide the optimal sterile:fertile ratios.

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Section: Discussionmentioning

confidence: 99%

Sexual Competitiveness and Induced Egg Sterility by Aedes aegypti and Aedes albopictus Gamma-Irradiated Males: A Laboratory and Field Study in Mexico

Bond

Aguirre-Ibáñez

Osorio

et al. 2021

Insects

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“…Reduced longevity following exposure to high doses of radiation have been reported previously in Ae . aegypti [47, 48] and Ae . albopictus [49, 50].…”

Section: Discussionmentioning

confidence: 99%

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

et al. 2019

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The sterile insect technique (SIT) may offer a means to control the transmission of mosquito borne diseases. SIT involves the release of male insects that have been sterilized by exposure to ionizing radiation. We determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of Aedes aegypti and Ae . albopictus in southern Mexico. The survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for either species. Irradiation had no significant adverse effects on the flight ability (capacity to fly out of a test device) of male mosquitoes, which consistently exceeded 91% in Ae . aegypti and 96% in Ae . albopictus . The average number of eggs laid per female was significantly reduced in Ae . aegypti at doses of 15 and 30 Gy and no eggs were laid by females that had been exposed to 50 Gy. Similarly, in Ae . albopictus , egg production was reduced at doses of 15 and 25 Gy and was eliminated at 35 Gy. In Ae . aegypti , fertility in males was eliminated at 70 Gy and was eliminated at 30 Gy in females, whereas in Ae . albopictus , the fertility of males that mated with untreated females was almost zero (0.1%) in the 50 Gy treatment and female fertility was eliminated at 35 Gy. Irradiation treatments resulted in reduced ovary length and fewer follicles in both species. The adult median survival time of both species was reduced by irradiation in a dose-dependent manner. However, sterilizing doses of 35 Gy and 50 Gy resulted in little reduction in survival times of males of Ae . albopictus and Ae . aegypti , respectively, indicating that these doses should be suitable for future evaluations of SIT-based control of these species. The results of the present study will be applied to studies of male sexual competitiveness and to stepwise evaluations of the sterile insect technique for population suppression of these vectors in Mexico.

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“…The study site, Batan Indah Residence, is suburban residential area located in Kademangan subdistrict, Setu district, South Tangerang Municipality, Banten Province. This location was chosen as study site due to high prevalence of Dengue fever cases in South Tangerang Municipality [18] and was already set as capture site for monitoring mosquito population from previous program conducted by BATAN. The Batan Indah Residence has a land area of 32 hectares and consist of around 1.000 household.…”

Section: Study Areamentioning

confidence: 99%

Estimation of Population Size and Dispersal Pattern of Sterile Male Aedes aegypti Using Mark-Release-Recapture (MRR)

et al. 2021

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Aedes aegypti is currently emerging as a main vector of Dengue, Zika, and Chikungunya transmission. Chemical control was reported to be less effective due to the resistance of this mosquito to some types of insecticides. Therefore, another vector control is needed which is most appropriate to be used, i.e. the sterile insect technique (SIT). Information about optimum range dispersal sterile male Aedes aegypti for optimalization SIT program are needed. This study was designed to determine the dispersal pattern and population estimation of Aedes aegypti sterilized with gamma rays using mark-release-recapture (MRR) method. After the male Aedes aegypti (pupal stage) was irradiated with 70 Gy of gamma rays, the mosquitoes were then marked with Rhodamine-B and released into the study site. MRR experiments were carried out in Batan Indah residential area, and the Aedes aegypti were released in center of the site. Mosquitoes were recaptured at 28 points spread over the Batan Indah Residence for 2, 4, 6, and 8 days after release by using BG-Sentinel Traps. The result showed that the population of Aedes aegypti in the site was estimated to be 5.402 (1.347-14.636; CI 95 %) with the furthest spread distance was 119 meters from the release point. This study also showed that the MRR experiment can be used to estimate the population size and dispersal pattern of Aedes aegypti movement in a given locality. The result of present study provide better understanding for optimalization mosquito-borne disease prevention based on SIT programs.

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Effects of gamma irradiation dose-rate on sterile male Aedesaegypti

Cited by 19 publications

References 25 publications

Sexual Competitiveness and Induced Egg Sterility by Aedes aegypti and Aedes albopictus Gamma-Irradiated Males: A Laboratory and Field Study in Mexico

Sexual Competitiveness and Induced Egg Sterility by Aedes aegypti and Aedes albopictus Gamma-Irradiated Males: A Laboratory and Field Study in Mexico

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

Estimation of Population Size and Dispersal Pattern of Sterile Male Aedes aegypti Using Mark-Release-Recapture (MRR)

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