Effects of gamma irradiation on the shoot length of Cicer seeds

Toker, Cengiz; Uzun, Bülent; Çancı, Hüseyin; Ceylan, Fatma Öncü

doi:10.1016/j.radphyschem.2005.03.011

Cited by 40 publications

(24 citation statements)

References 7 publications

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“…This observation could be correlated with the results obtained by Khalil et al (1986), who attributed a decreased in shoot and root lengths at higher doses of gamma rays resulted from reduction mitotic activity in meristematic tissues and reduced moisture contents in seeds. The decrease in morphological characteristics of various crops has already reported by (Thimmaiah et al, 1998;Khan and Qureshi, 2001;Al-Salhi et al, 2004 andToker et al, 2005), which supports the present investigation.…”

Section: Morphological Analysissupporting

confidence: 92%

Effect of Gamma-radiation Doses of Phenotypic and Molecular Characteristics of two Egyptian Soybean Varieties

et al. 2017

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WO Egyptian soybeans varieties, Giza111 and Crawford were selected for the current study. Their seeds were exposed to three doses of gamma-radiation 150, 200, and 250 Gy in order to study their mutagenic effects in the treated seeds and two following generations at various levels. In the first generation (M1), two doses (150 and 200 Gy) were the most effective in enhancement of some agronomic traits like number of stem branches, number of pods per plant, number of seeds per pod and weight of 100 seeds and crop yield. Analysis of protein profiles using SDS-PAGE showed variation among the treatments in each variety, where a total of 35 protein bands were recorded. Three of which were unique bands, one unique band of size 37 kDa was found in the treated seeds before germination (M0) in Crawford at 250 Gy treatment. The other two unique bands (102 and 162 kDa) were found in M2 generation in Crawford at 150 Gy and Giza111 at 200 Gy, respectively. ISSR analysis using five primers gave a total 45, 38 and 28 ISSR bands in M0, M1 and M2, respectively; 4 of which were unique bands. The primer HB14 amplified 6 DNA bands, 2 were unique bands (895 &1050 bp), both in M1 generation of Giza111 at 150 Gy, while primer 844 and 862 amplified only one unique band both found in M0 Giza111 control. They have a molecular size of 888 and 900 bp, respectively. Although, UBC-827 amplified the highest number of bands (7) no unique bands were found.Keywords: Glycine max L., Gamma-radiation, SDS-PAGE, ISSR, polymorphism.Soybean (Glycine max L.) integrates in one crop both the dominant world supply of edible vegetable oil, and the dominant supply of high-protein feed supplements for livestock. Its seed residues and derivatives have a substantial economic importance in a wide range of industrial, food, pharmaceutical, and agricultural products (Smith and Huyser, 1987). In addition, soybean protein is rich in the valuable amino acid lysine (5%) in which most of the cereals are deficient. Moreover, it contains a good quantity of minerals, salts and vitamins. Soybean's oil has been used in Egypt since year 1976, its oil extraction ratio is about 20.5%. Furthermore, soybean is an essential source of protein in the animal and poultry feed (Çelik et al., 2014).Mutations, both spontaneous and induced have been highly successful in changing the fatty acid composition of several oil seed crops. Three reasons have been given for attempts in changing seed oil quality by means of single gene mutations being exceptionally successful. Various soybean mutant lines were identified using mutation, which possessed many desirable traits like habit, low linolenic, high oleic content and resistance to pod shattering (Rahman et al., 1995). In addition, mutational breeding is thought to be as a source of increasing genetic variability and particular improvement could be conferred without importantly changing its satisfactory phenotype (Mudibu et al., 2011; Ambavane et al., 2015(. In plants, induction of genetic and morphological variabilities, using induced mutati...

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Section: Morphological Analysissupporting

confidence: 92%

Effect of Gamma-radiation Doses of Phenotypic and Molecular Characteristics of two Egyptian Soybean Varieties

et al. 2017

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“…Kumar and Mishra (2004) noted that in okra (Abelmoschus esculentus), germination percentage generally decreased with the increasing doses of gamma rays. Reduction in germination percentage with increasing dose of gamma radiation has also been reported in Pinus (Thapa, 2004), Rye (Akgun and Tosum, 2004), Chickpea (Khan et al, 2005), Cicer (Toker et al, 2005) and Citrus jambhiri Lush. (Sharma et al, 2013;Saini and Gill, 2009;Kaur and Rattanpal, 2010).…”

Section: Results and Disscusionmentioning

confidence: 72%

Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.)

Kaur

2015

JANS

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Abstract:The present study on the effect of mutagens on regeneration and growth of in vitro grown epicotyls segments of rough lemon seedlings (Citrus jambhiri Lush.) was carried out during the years 2009-2010 and 2010-2011 in the Tissue Culture Laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Developmental characteristics of the in vitro grown epicotyls segments on regeneration media were treated employing gamma radiation in Gray(Gy) at 0, 5,10,15,20,25,30,35,40 and 45 Gy; the alkylating agent ethyl methane sulfonate (EMS) and methyl methane sulfonate (MMS) each at 0, 0.1, 0.2, 0.3, 0.4,0.5 and 0.6% (v/v) were evaluated. Epicotyl segments from one month old in vitro grown seedling were cultured in regeneration medium (MS+BAP 1.0 mglit -1 ) under controlled laboratory conditions (25±2⁰ C, 16 hr photoperiod, 2000 lux light). LD 50 , the dose required to kill half of the tested population corresponded to 35Gy for gamma radiation, 0.3% each for EMS and MMS treatments. Number of days taken for regeneration increased with increasing dose of gamma irradiation, EMS and MMS. Percent regeneration, number of buds, number of shoots, shoot length, number of leaves, internodal length, primary root length and number of secondary roots decreased with increasing dose of gamma radiation , EMS and MMS. The study would be beneficial to induce desirable variations in plant growth characteristics of rough lemon by the use of mutagens treatment.

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“…Several workers (Akgun and Tosum, 2004;Alcantara et al, 1996;Karthika and Lakshmi, 2006;Khan et al, 2005;Kumar and Mishra, 2004;Thapa, 2004;Toker et al, 2005) reported such behavior of physical and chemical mutagens in different crop plants. Taking into the account of the findings documented by various workers (Chaghtai and Prasad, 1979;Gichner and Veleminsky, 1974;Jain and Agarwal, 1987;Mensah et al, 2007;Nazir et al, 2005;Santos, 1961;Sareen and Koul, 1999) and it is inferred that presoaking renders the seed vulnerable to the effect of physical and chemical mutagens and the germination is inhibited due to biochemical interference of physical and chemical mutagens The reduction in plant survival percentage following with physical and chemical mutagens has been reported by many workers, and results of present investigation regarding survival are in agreement with earlier findings in different plants (Bari, 1971;Cheema and Atta, 2003;Davies et al, 1975;Hussein and Disouki 1975;Kar et al, 1995;Siddiq and Swaminathan, 1968;Singh et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Physical and Chemical Mutagenesis in Linum usitatissimum L. to Induce Variability in Seed Germination, Survival and Growth rate traits

Bhat

Pandit

Sheikh

et al. 2017

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The studies of induced mutation in Linum usitatissimum L. were performed by exposing the fully matured and healthy dry seeds to gamma rays at 10 and 15 Krad (Kr), X rays at 10 and 15 Kr, hydroxylamine (HA) at 0.1% and 0.2%, and 5 amino acridine (AA) at 0.1% and 0.2% doses. The observations were made for seed germination percentage, survival percentage, and growth characters such as shoot length, root length, and dry weight. Seeds treated with low dose of mutagens showed negligible effect while that with the high dose exhibited significant effects on studied parameters as compared to control. Data obtained in this study were statistically significant at 5% level. The results conclude that treatments of gamma rays and X-rays were less effective as compared to those of HA and AA treatments.KEY WORDS: Acridine, hydroxylamine, Linum usitatissimum L., mutation Current Botany 2016, 7: 28-32 http://scienceflora.org/journals/index.php /cb/ doi: 10.19071/cb.2016.v7.3054 Original Article Bhat, et al.: Effect of physical and chemical mutagens on seeds of Linum usitatissimum L.

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Effects of gamma irradiation on the shoot length of Cicer seeds

Cited by 40 publications

References 7 publications

Effect of Gamma-radiation Doses of Phenotypic and Molecular Characteristics of two Egyptian Soybean Varieties

Effect of Gamma-radiation Doses of Phenotypic and Molecular Characteristics of two Egyptian Soybean Varieties

Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.)

Physical and Chemical Mutagenesis in Linum usitatissimum L. to Induce Variability in Seed Germination, Survival and Growth rate traits

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