Effects of gamma irradiation under vacuum and air packaging atmospheres on the phytochemical contents, biological activities, and microbial loads of Kaempferia parviflora rhizomes

Krongrawa, Wantanwa; Limmatvapirat, Sontaya; Saibua, Supachai; Limmatvapirat, Chutima

doi:10.1016/j.radphyschem.2020.108947

Cited by 13 publications

(12 citation statements)

References 23 publications

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“…Previously published papers have also indicated that levels of total flavonoids are changed under in vitro culture conditions [ 49 , 50 , 51 ]. Krongrawa et al [ 52 ] reported that levels of total phenolics and flavonoids in K. parviflora are 17.88–19.07 mg GAE/g and 15.90–16.68 mg QE/g, respectively, after gamma radiation. In addition, Choi et al [ 53 ] reported that the contents of total phenolics in different fractions of K. parviflora are 19.48–92.26 mg GAE/g extract.…”

Section: Resultsmentioning

confidence: 99%

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Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Park

Kim

et al. 2021

Plants

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This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L−1 AgO NPs for 60 min eliminated contamination without affecting the survival of explants. The number of shoots (12.2) produced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 µM of 6-Benzyladenine (6-BA) and 0.5 µM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 µM of Indole-3-butyric acid (IBA). A survival rate of 98% was attained when plantlets of K. parviflora were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine the chemical profile of K. parviflora leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse-grown) plantlets of K. parviflora. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase inhibitory abilities. These results suggest that leaves of K. parviflora, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

“…Several studies have reported antioxidant properties of K. parviflora extracts or fractions. For example, Krongrawa et al [ 52 ] demonstrated antioxidant properties of K. parviflora extracts using DPPH and FRAP assays. In their study, IC 50 values ranged from 129.08 µg/mL to 165.26 µg/mL in the DPPH assay.…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Park

Kim

et al. 2021

Plants

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“…Anti-inflammatory Activities. Inhibition of protein denaturation was determined according to our previous procedure [27], and the inhibitory activities were expressed as IC 50 (mg/ml), the concentration of the extract producing 50% inhibition of the protein denaturation. Briefly, the reaction mixture was comprised of 0.2 ml of fresh egg albumin, 2.8 ml of phosphate buffered saline (pH 7.4), and 2 ml of sample solution with a concentration range varying between 0.2 and 4 mg/ml.…”

Section: Fourier-transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Preparation and Properties of Anti-Nail-Biting Lacquers Containing Shellac and Bitter Herbal Extract

Limmatvapirat

Chansatidkosol

et al. 2021

International Journal of Polymer Science

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The purpose of the present investigation was to formulate and evaluate anti-nail-biting lacquers consisting of bitter herbal extracts. The hydroalcoholic extracts obtained from Andrographis paniculata and Tinospora crispa were determined for phytochemical constituents, total phenolic contents, antioxidant activities, anti-inflammatory activities, and cytotoxicities. Anti-nail-biting lacquers were prepared by using herbal extracts (bittering agent), shellac (film forming polymer), ethanol (volatile solvent), and other indispensable additives with continuous stirring. Thus, attempts to enhance the film property and bitterness are accomplished by using polyvinylpyrrolidone (PVP K30) as a copolymer and varying concentrations of herbal extracts. Good accepted formulations were established for drying time, pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva and evaluated their bitterness in human volunteers. The results revealed that phytochemical constituents including tannins, glycosides, reducing sugars, alkaloids, terpenoids, and flavonoids were found present in both extracts while saponins were only detected in A. paniculata extract. Although T. crispa extract exhibited a significantly higher ( p < 0.05 ) total phenolic content and antioxidant activity than A. paniculata extract, it showed lower protein denaturation inhibition property than A. paniculata extract. Because of the potentials of both extracts without cytotoxicity, anti-nail-biting lacquers containing either A. paniculata extract or T. crispa extract were developed and evaluated. Drying time of formulations was 6-11 min with visually seen glossiness of formulation. Formulations of the nail lacquer showed good pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva. The formulations displaying no significant cytotoxicity effect on CRL-2076 cells were assessed on healthy human volunteers to compare bitterness and film characteristics. The results revealed that the optimized formulation containing A. paniculata extract could successfully achieve good film forming property and bitterness release which is considered promising for stopping nail biting.

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“…Inhibition of protein denaturation was determined according to our previous procedure [27] and the inhibitory activities were expressed as IC 50 (mg/ml), the concentration of the extract producing 50% inhibition of the protein denaturation. Brie y, the reaction mixture was comprised of 0.2 ml of fresh egg albumin, 2.8 ml of phosphate buffered saline (pH 7.4), and 2 ml of sample solution with a concentration range varying between 0.2 and 4 mg/ml.…”

Section: Anti-in Ammatory Activitiesmentioning

confidence: 99%

Extraction, Biological Activity, Formulation, and Evaluation of Bitter Herbs as Anti-Nail-Biting Lacquers

Limmatvapirat

Chansatidkosol

et al. 2021

Preprint

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Background: Nail biting can increase the risk of infection and abnormal-looking nails. The purpose of the present investigation was to formulate and evaluate anti-nail-biting lacquers consisting of bitter herbal extracts. Methods: The hydroalcoholic extracts obtained from Andrographis paniculata and Tinospora crispa were determined for phytochemical constituents, total phenolic contents, antioxidant activities, anti-inflammatory activities, and cytotoxicities. Anti-nail-biting lacquers were prepared by using herbal extracts (bittering agent), shellac (film forming polymer), ethanol (volatile solvent), and other indispensable additives with continuous stirring. Thus, attempts to enhance the film property and bitterness is accomplished by using polyvinylpyrrolidone K30 as a copolymer and varying concentrations of herbal extracts. Good accepted formulations were established for drying time, pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva and then evaluated their bitterness in human volunteers.Results: Phytochemical constituents including tannins, glycosides, reducing sugars, alkaloids, terpenoids, and flavonoids were found present in both extracts while saponins was only detected in A. paniculata extract. Although T. crispa extract exhibited a significantly higher (p < 0.05) total phenolic content and antioxidant activity than A. paniculata extract, it showed lower protein denaturation inhibition property than A. paniculata extract. Because of the potentials of both extracts without cytotoxicity, anti-nail-biting lacquers containing either A. paniculata extract or T. crispa extract were developed and evaluated. Drying time of formulations were 6-11 min with visually seen glossiness of formulation. Formulations of the nail lacquer showed good pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva. The formulations displaying no significant cytotoxicity effect on CRL-2076 cells were assessed on healthy human volunteers to compare bitterness and film characteristics. Conclusion: The optimized formulation containing A. paniculata extract could successfully achieve good film forming property and bitterness release which is considered promising for stopping nail biting.

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Effects of gamma irradiation under vacuum and air packaging atmospheres on the phytochemical contents, biological activities, and microbial loads of Kaempferia parviflora rhizomes

Cited by 13 publications

References 23 publications

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Preparation and Properties of Anti-Nail-Biting Lacquers Containing Shellac and Bitter Herbal Extract

Extraction, Biological Activity, Formulation, and Evaluation of Bitter Herbs as Anti-Nail-Biting Lacquers

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