Effects of gamma radiation on hard dental tissues of albino rats: investigation by light microscopy

Abstract

The present work aims at studying the effect of gamma radiation on the hard dental tissues. Eighty adult male albino rats with weights of about 250 g were used. The rats were irradiated at 0.2, 0.5, 1.0, 2.0, 4.0 and 6.0 Gy whole-body gamma doses. The effects on hard dental tissue samples were investigated after 48 h in histological and ground sections using light microscopy. Areas of acid phosphatase activity were detected using tartrate-resistant acid phosphatase (TRAP) stains. Observation of histological sections revealed disturbance in predentin thickness and odontoblastic layer as the irradiation dose increased. In cementum, widened cementocytes lacunae were occasionally detected even with low irradiated doses. On the other hand, relatively homogenous enamel was detected with darkened areas in enamel surface at doses over than 0.5 Gy. TRAP-positive cells were detected on the surface of the dentin of irradiated groups as well as cementum surface. Minimal detectable changes were observed in ground sections.

Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries

“…Furthermore, morphological preservation of the enamel components, including lamellae, spindles, and tufts, which are considered to play an important role in terms of adherence capacity and mechanical strength between the enamel and the dentin, seemed to be unchanged by radiation on optical light microscopy analysis, as were the other expected unchanged enamel and dentin-pulp complex developmental components. The histologically normal odontoblast morphology, the palisade construction of the odontoblast cell layer, and the unaffected pulp tissue confirm similar findings of previous studies [El-Faramawy et al, 2013;Faria et al, 2014], as well as the formation of reparative tertiary dentin in response to caries that has also been demonstrated previously [Silva et al, 2009]. Together, these findings strongly indicate that in post-HNRT teeth the pulp not only remains vital but also retains its capacity to respond to external irritation, such as caries.…”

ATR-FTIR Assessment of the Biochemical Composition and Micro Hardness of the Hard Tissues of Oral Cavity Submitted to Gamma Irradiation

“…Some studies [9][10][11][12][13][14][15][16][17][18][19][20][21] report unfavorable results in the tissues of oral cavity with greater organic content after radiotherapy treatment; however, it is necessary to analyze the amount of mineral loss that occurs in the hard tissues submitted to gamma irradiation [22].…”

Histological and immunohistochemical evaluation for the effect of pilocarpine and quercetin on gamma-irradiated parotid salivary glands

“…During the last decades, there has been a substantial improvement in the treatment of head and neck cancer and radiotherapy is currently a widely used and important part of this treatment (El-Faramawy et al, 2013). In comparison with the surgical procedures, it shows better results because it can be used as a curative, adjuvant, neoadjuvant and palliative type of treatment and is often used in the conservative approaches, with protocols that preserve the organs and tissues (Marta et al, 2014).…”