Effects of gamma radiation on the early developmental stages of Zebrafish ( Danio rerio )

Kumar, Munish; Shyama, S.K.; Shamim, Kashif; Dubey, Santosh Kumar; D’Costa, Avelyno H.; Sonaye, B.; Samit, B. Kadam; Chaubey, R.C.

doi:10.1016/j.ecoenv.2017.03.054

Cited by 27 publications

(10 citation statements)

References 47 publications

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“…However, the preceding experiment described in the present work makes use of zebrafish embryos at pharyngula stage for a first in vivo study of the proton Flash effect in general. Embryos at this stage are robust enough to resist volume-restriction during the experiment, but are concurrently less susceptible to radiation than embryos at earlier developmental stages [16,17]. For example, the electron Flash effect was recently observed for 4 hpf zebrafish embryos [17], whereas 24-28 hpf embryos were applied in the present study.…”

Section: Discussionmentioning

confidence: 57%

Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

Beyreuther

Brand

Hans

et al. 2019

Radiotherapy and Oncology

183

159

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Section: Discussionmentioning

confidence: 57%

Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

Beyreuther

Brand

Hans

et al. 2019

Radiotherapy and Oncology

183

159

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“…The slightly reduced hatching rates of 86–90%, which were noticed at the second day after photon treatment could either be explained by the photon treatment itself or be a consequence of the delayed embryo development. Generally, most of the literature also shows a rather unaffected hatching rate after radiation treatment, which indicates that zebrafish embryos at pharyngula stage are quite radioresistant compared to those irradiated at earlier developmental stages e.g., [ 16 , 26 – 28 ]. Few exceptions were published by Hu et al [ 27 ] and Li et al [ 29 ] who observed lower hatching rates two days after irradiation of 24 hpf embryos with 1 Gy of 137 Cs photons [ 27 ] and 12 Gy of 8 MeV protons [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the latter, longer observation times, like for example the 7 dpi applied by Szabo et al [ 16 ] after 20 Gy MV photon exposures, are required to notice the respective reduction in survival to 50% of the embryos. On the other side, embryos at earlier developmental stages than the 24 hpf used in the present work are more affected by radiation as shown by a mortality rate of 73.3% after 10 Gy 60 Co gamma-irradiation of embryos at 3 hpf [ 28 ]. One potential explanation of the radioresistance of zebrafish embryo during pharyngula stage was provided by Hu et al [ 27 ], who observed higher levels of proteins required for the defense against reactive oxygen species in 24 hpf compared to 6 hpf embryos.…”

Section: Discussionmentioning

confidence: 99%

Radiobiological effects and proton RBE determined by wildtype zebrafish embryos

et al. 2018

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The increasing use of proton radiotherapy during the last decade and the rising number of long-term survivors has given rise to a vital discussion on potential effects on normal tissue. So far, deviations from clinically applied generic RBE (relative biological effectiveness) of 1.1 were only obtained by in vitro studies, whereas indications from in vivo trials and clinical studies are rare. In the present work, wildtype zebrafish embryos (Danio rerio) were used to characterize the effects of plateau and mid-SOBP (spread-out Bragg peak) proton radiation relative to that induced by clinical MV photon beam reference. Based on embryonic survival data, RBE values of 1.13 ± 0.08 and of 1.20 ± 0.04 were determined four days after irradiations with 20 Gy plateau and SOBP protons relative to 6 MV photon beams. These RBE values were confirmed by relating the rates of embryos with morphological abnormalities for the respective radiation qualities and doses. Besides survival, the rate of spine bending, as one type of developmental abnormality, and of pericardial edema, as an example for acute radiation effects, were assessed. The results revealed that independent on radiation quality both rates increased with time approaching almost 100% at the 4th day post irradiation with doses higher than 15 Gy. To sum up, the applicability of the zebrafish embryo as a robust and simple alternative model for in vivo characterization of radiobiological effects in normal tissue was validated and the obtained RBE values are comparable to previous finding in animal trials.

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“…Apart from using zebrafish as an in vivo model system, in addition to the zebrafish embryo model that can be regarded as an in vitro method, the use of zebrafish cultured cell lines in genetic toxicology is also on the rise [91,[97][98][99][100][101]. Up to now there have been more than 100 papers describing the zebrafish model both in vivo and in vitro using the comet assay for the assessment of different physical and/or chemical agents such as gamma rays [102,103], X-rays [104], pesticides [105], insecticides [106], fungicides [88,107], herbicides [108], mycotoxins [109], pharmaceuticals [98,99,[110][111][112], heavy metals A c c e p t e d M a n u s c r i p t 9 [108,113,114], PAHs [89], nanoparticles [115][116][117][118], flame retardants [119], sewage effluent [120], waste material [121] as well as model toxicants such as benzo(a)pyrene (B[a]P), methyl methanesulfonate (MMS) and ethyl methanesulfonate (EMS) [122,123].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Effects of gamma radiation on the early developmental stages of Zebrafish ( Danio rerio )

Cited by 27 publications

References 47 publications

Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

Radiobiological effects and proton RBE determined by wildtype zebrafish embryos

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

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