Effects of gamma radiation (γ) on biochemical and antioxidant properties in black gram (<i>Vigna mungo</i>L. Hepper)

Yasmin, K.; Arulbalachandran, D.; Soundarya, V; Vanmathi, S

doi:10.1080/09553002.2019.1589022

Cited by 22 publications

(13 citation statements)

References 41 publications

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“…The present results reveal that gamma irradiation might induce physiological activity disturbances, arrest the cell division, change or inhibit enzyme activity. Similar results were observed in many crops such as cowpea (36)(37)(38), black gram (11,39,40), soybean (41) and horse gram (42). Whereas the days to first flowering were delayed in all the irradiated seeds, particularly in 800 Gy, which took more days (41.76 ± 0.15) compared to control.…”

Section: Discussionsupporting

confidence: 78%

“…The physical and chemical mutagenesis creates genetic variation, produces new varieties with enhancing characteristics (4). Mutation breeding can be a valuable technique to traditional breeding methods and have been successful in the improvement of qualitative and quantitative traits in many crops such as cowpea (5)(6)(7), soybean (8,9), black gram (10)(11)(12). Moreover, mutation breeding takes less time, easy to handle and is highly useful in producing crop cultivars than traditional breeding methods (13,14).…”

Section: Introductionmentioning

confidence: 99%

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Effects of gamma radiation on quantitative traits and genetic variation of three successive generations of cowpea (Vigna unguiculata (L.) Walp.)

2021

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An annual pulse crop cowpea (Vigna unguiculata (L.) Walp.), commonly named southern pea, is a nourishing constituent for the human diet and fodder. Gamma rays are a potent mutagenic agent to stimulate genetic variation with better characteristics, improving the yield relating traits in crops. Hence, the present study focused on exploring genetic variation between three generations in the mutant populations of cowpea through SCoT markers. The mutant populations of three successive generations, M1, M2 and M3, were induced by different doses [200, 400, 600, 800, 1000 and 1200 Gray (Gy)] gamma irradiation. The results depict that the quantitative characters were reduced by increasing the dosage of gamma irradiation in the M1 generation. In contrast, the second and third generation of plants showed a significant increase in yield and yield contributing traits than control and the maximum increase was noticed at 200 Gy and 400 Gy. Days to first flowering was delayed in irradiated plants than control of M1 generation. In contrast, in consecutive generations (M2 and M3), the early first flowering was noticed at 400 Gy and late flowering was observed at 800 Gy compared respectively to control and other doses. Seed yield per plant mean value was increased at 200 Gy in both generations (M2 and M3); it may produce new genotypes to desirable traits such as yield and quality. SCoT markers were used to explore genetic variation at the genomic level of mutant populations and screened with eight primers. Among them, seven primers showed amplification of 222 bands, in which 133 bands showed polymorphism. The polymorphic bands varied from 3.03–96.07%. The genetic variation, such as the number of different alleles (Na), effective number of alleles (Ne), Shannon’s information index (I), expected heterozygosity (He) and unbiased expected heterozygosity (uHe) showed an average value of 1.352 ± 0.092, 1.278 ± 0.027, 0.293 ± 0.023, 0.184 ± 0.016, and 0.194 ± 0.016, respectively. AMOVA depicted significant genetic variation between all generations and indicated a total of 95% within populations and 5% among population variation by the marker used. The present investigations prominently showed that the variations induced by gamma irradiation were inherited from successive generations of the improvement in cowpea quantitative traits. This investigation gives acceptable proof that the SCoT markers are a valuable tool to identify the genetic variation among the three generations of cowpea.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Effects of gamma radiation on quantitative traits and genetic variation of three successive generations of cowpea (Vigna unguiculata (L.) Walp.)

2021

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“…Contrary to our results, seed germination was reduced to all doses of sodium azide in The results revealed that there is a linear association between increased doses of mutagens (SA and EMS) and reduced germination of seeds. This was also reported in black gram[20], wheat[21] and Okra[22]. The shoot length in two okra varieties was observed to exhibit marginal differences in mutagens treated and control.…”

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confidence: 71%

Effect of chemical mutagens on growth of Okra (Abelmoschus esculentus L. Moench)

Nizamani

Rafiq

Kaleri

et al. 2020

PAB

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The purpose of the present study was to evaluate the effects of mutagens i.e. Ethyl methanesulfonate (EMS) and Sodium azide (SA) on the morphological growth of Okra. Seeds of two Okra varieties Subzpari and Pahuja were first pre-soaked in distilled water and then treated with 0.0 (Control), 0.1, 0.2 and 0.3% Sodium azide (SA) and Ethyl methanesulfonate (EMS) separately. The results showed that the germination rate was significantly decreased with increased lethality (%) and mutation frequency (%) in M1 of both varieties with increasing concentrations of EMS and SA against control. The results showed that different concentrations of EMS and SA affected morphological attributes of both okra varieties as the increase of mutagen concentration showed variable results in M1 generation compared with control, but M2 generation showed useful improvement in various traits. Such as the M2 generation of Subs pari from 0.1% SA treatment showed a decrease in flowering time while an increase in the number of fruits per plant, fruit size, seeds yield (g) per plant and 100 seeds weight.

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“…The grains contain a high protein content of 20-30%. This protein is rich in amino acids lysine and tryptophan (Steele, 1976;Yasmin, Arulbalachandran, Soundarya, & Vanmathi, 2019).…”

Section: Introductionmentioning

confidence: 99%

Morphological, agronomical and molecular characterization in irradiated Cowpea (Vignaunguiculata (L.) Walp.) and detection by start codon target markers

Ezzat

Adly

El-Fiki

2019

Journal of Radiation Research and Applied Sciences

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Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important foods and economic vegetable crops in the world. Plant breeders resorted to using mutagenesis, especially gamma radiation to improve the yield production and protein content. Two Egyptian cowpea varieties Dokii 331 and Kaha 1 were exposed to different doses of gamma rays (0, 50, 100, 150, 200, 250 and 300 Gy) to obtain mutations of economic value and to identify genetic variation by SCoT markers. The exposure of cowpea CVs. Dokii 331 and Kaha 1 to these gamma radiation doses had a positive effect on the early flowering, increase of the number of primary branches number of flowers per plant, pods per plant and the number of seeds, and consequently crop quantity. The dose of 150 Gy was effective in obtaining a dwarf mutation in cv. Kaha 1 where the length of the plant reached 33.4 cm. While in cv. Dokki 331, the dose of 250 Gy induced a change in size and pod color and increase number of pods per plant. While the effect of dose 300 Gy caused to change the color of flower from white to violet and plant length until it reached to 277.4 cm. However, the dose 300 Gy caused changing in the flower color and plant length reached 277.4 cm. Genetic variation in both irradiated cultivars was determined by SCoT markers analysis. The isolated DNAs from irradiated plants were amplified by fifteen SCoT primers. It was obtained 219 bands identified in both cultivars. Dakii 331 (105) Band was given by Polymorphic band number 46 (43.81%). Kaha 1 was 114 Band by Polymorphic band number 55(48.25%). Some SCoT primers were able to generate with a total of 26 specific markers. The cluster analysis based on Jacquard's similarity coefficients and UPGMA algorithms were calculated.

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Effects of gamma radiation (γ) on biochemical and antioxidant properties in black gram (Vigna mungoL. Hepper)

Cited by 22 publications

References 41 publications

Effects of gamma radiation on quantitative traits and genetic variation of three successive generations of cowpea (Vigna unguiculata (L.) Walp.)

Effects of gamma radiation on quantitative traits and genetic variation of three successive generations of cowpea (Vigna unguiculata (L.) Walp.)

Effect of chemical mutagens on growth of Okra (Abelmoschus esculentus L. Moench)

Morphological, agronomical and molecular characterization in irradiated Cowpea (Vignaunguiculata (L.) Walp.) and detection by start codon target markers

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