Effects of Garcinia cambogia Extract on the Adipogenic Differentiation and Lipotoxicity

강은실,; 함선아,; 황정석,; 이창권,; 서한극,

doi:10.5851/kosfa.2013.33.3.411

Cited by 15 publications

(6 citation statements)

References 21 publications

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“…Our results showed that (-)-HCA treatment significantly reduced the amount and total area of lipid droplets in cultured primary chicken hepatocytes. These results were consistent with the previous report which showed that Garcinia cambogia extracts significantly suppressed the adipogenic differentiation of pre-adipocytes and intracellular lipid accumulation in the differentiating adipocytes [66]. The previous study reported that the Garcinia cambogia extracts inhibits lipid droplet accumulation in fat cells without affecting adipose conversion in 3T3-L1 cells [67].…”

Section: Discussionsupporting

confidence: 93%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism.

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Section: Discussionsupporting

confidence: 93%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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“…In addition, G. cambogia extract inhibited adipogenic differentiation in 3T3-L1 adipocytes by suppressing the expression of PPARγ and C/EBPα (E. S. Kang, Ham, Hwang, Lee, & Seo, 2013), which is consistent with our results (Figure 1d). However, our study also shows the inhibitory effect of G. cambogia on preadipocyte proliferation and MCE (Figures 2 and 3).…”

Section: Discussionsupporting

confidence: 92%

Garcinia cambogia suppresses adipogenesis in 3T3‐L1 cells by inhibiting p90RSK and Stat3 activation during mitotic clonal expansion

Han

Jang

Park

et al. 2020

Journal Cellular Physiology

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Obesity is associated with an increase in adipose tissue, which is mediated by hyperplasia and hypertrophy. Therefore, inhibiting cell proliferation during mitotic clonal expansion (MCE) is one of the major strategies for preventing obesity. The antagonistic effects of Garcinia cambogia (G. cambogia) on obesity have been studied in animal experimental models. However, the effects of G. cambogia extract on MCE, and the underlying molecular mechanisms, are poorly understood. In this study, 3T3-L1 cells were used to investigate whether G. cambogia extract affected cell proliferation during MCE and to identify target molecules for any antiadipogenic activity. G. cambogia extract suppressed isobutylmethylxanthine and dexamethasone-and-insulin (MDI)-induced adipogenesis at an early stage by attenuating MCE. In G. cambogia extract-treated preadipocytes, MDI-induced cell proliferation and cell cycle progression were inhibited by G 0 /G 1 arrest due to an increase in p21 and p27 expression, and inhibition of cyclin-dependent kinase 2, cyclin E1 expression, and retinoblastoma (Rb) phosphorylation. In addition, the MDI-induced phosphorylation and subsequent translocation into the nucleus of p90 ribosomal S6 kinase (p90RSK) and signal transducer and activator of transcription (Stat) 3 were suppressed. Specific inhibitors of p90RSK (FMK) and Stat3 (stattic) regulated cell proliferation and adipogenesis. In conclusion, this study demonstrated that G. cambogia extract inhibited MCE by regulating p90RSK, Stat3, and cell cycle proteins, leading to G 0 /G 1 arrest. These findings provide new insight into the mechanism by which G. cambogia suppresses adipocyte differentiation and show that p90RSK is critical for adipogenesis as a new molecular target.

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“…In in vitro studies, Garcinia extract (1 mg/mL) treatment inhibited lipid droplet accumulation in 3T3-L1 preadipocytes (90). Similarly, Garcinia extract was found to inhibit cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocytes (91,92).…”

Section: Garcinia Cambogia (Hydroxycitric Acid) the Effect Of Hydroxymentioning

confidence: 93%

Nutrition Supplements to Stimulate Lipolysis: A Review in Relation to Endurance Exercise Capacity

Kim

Park

Lim

2016

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Athletes make great efforts to increase their endurance capacity in many ways. Using nutrition supplements for stimulating lipolysis is one such strategy to improve endurance performance. These supplements contain certain ingredients that affect fat metabolism; furthermore, in combination with endurance training, they tend to have additive effects. A large body of scientific evidence shows that nutrition supplements increase fat metabolism; however, the usefulness of lipolytic supplements as ergogenic functional foods remains controversial. The present review will describe the effectiveness of lipolytic supplements in fat metabolism and as an ergogenic aid for increasing endurance exercise capacity. There are a number of lipolytic supplements available on the market, but this review focuses on natural ingredients such as caffeine, green tea extract, L-carnitine, Garcinia cambogia (hydroxycitric acid), capsaicin, ginseng, taurine, silk peptides and octacosanol, all of which have shown scientific evidence of enhancing fat metabolism associated with improving endurance performance. We excluded some other supplements owing to lack of data on fat metabolism or endurance capacity. Based on the data in this review, we suggest that a caffeine and green tea extract improves endurance performance and enhances fat oxidation. Regarding other supplements, the data on their practical implications needs to be gathered, especially for athletes.

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Effects of Garcinia cambogia Extract on the Adipogenic Differentiation and Lipotoxicity

Cited by 15 publications

References 21 publications

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Garcinia cambogia suppresses adipogenesis in 3T3‐L1 cells by inhibiting p90RSK and Stat3 activation during mitotic clonal expansion

Nutrition Supplements to Stimulate Lipolysis: A Review in Relation to Endurance Exercise Capacity

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