Effects of genetic background on responses to superovulation in Japanese Black cattle

Hirayama, Hiroki; Naito, Akira; Fujii, Takashi; Sugimoto, Masahito; Takedomi, T.; Moriyasu, Satoru; Sakai, Hitomi; Kageyama, Soichi

doi:10.1292/jvms.18-0537

Cited by 17 publications

(20 citation statements)

References 14 publications

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“…Mutations in the GRIA1 gene have been described to be involved with superovulation rates in Wagyu cattle [ 14 ]. In addition to that, a study conducted by Hirayama and colleagues [ 15 ] identified that levels of plasma anti-Müllerian hormone (AMH) concentration, age in months, repeated superovulation, mutations in the FSHR gene and bloodlines had significant effects on the responses to superovulation. To minimize the effect of the genetic variation in response to SOV protocols, we used only animals with a greater percentage of the Tajima line.…”

Section: Discussionmentioning

confidence: 99%

“…Sugimoto et al [ 14 ] have identified an association of a single nucleotide polymorphism (SNP) in the glutamate ionotropic receptor AMPA type subunit 1 ( GRIA1 ) with ovulation rates in Wagyu cattle. Hirayama et al [ 15 ] have further characterized variations in the allele frequency of GRIA1 among different lines of Wagyu, indicating an effect of the genetic background on the responses to superovulation. Therefore, the selection of animals with the allele favorable to a superovulation protocol (SOV) response might improve reproductive success in Wagyu cattle.…”

Section: Introductionmentioning

confidence: 99%

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The Outcome and Economic Viability of Embryo Production Using IVF and SOV Techniques in the Wagyu Breed of Cattle

Facioli

Marchi

Marques

et al. 2020

Veterinary Sciences

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The Japanese black cattle breed (Wagyu) has an improved metabolism, which allows them to have a higher marbling score when compared with other cattle breeds. However, this may affect other aspects of the animal’s physiology, including hormone secretion and their reproductive success, such as their response to synchronization protocols and embryo production. Therefore, the objectives of this study were to test a superovulation protocol (SOV) developed with low doses of FSH and to evaluate the outcome and economic viability of embryo production using the SOV and in vitro fertilization (IVF) approaches in the Wagyu cattle breed. For that, ten Wagyu cows were submitted to five SOVs over a period of 15 months using a standard protocol: CIDR + 3 mg estradiol benzoate (D0), 35 mg FSH (Folltropin®) a.m. and p.m. (D4), 35 mg Folltropin® a.m. and 20 mg p.m. (D5), 20 mg Folltropin® a.m. and 10 mg p.m. (D6), 10 mg Folltropin® and 0.5 mg cloprostenol, both a.m. and p.m., + CIDR removal (D7), 0.05 mg GnRH + insemination 12 and 24 h after (D8) and embryo collection + 0.5 mg of cloprostenol (D16). Thirty days after each SOV, a follicular aspiration was conducted to produce IVF embryos without any pre-synchronization using standard semen in the same group of animals. The average number of embryos produced was 7.63 ± 5.61 (SOV) and 4.52 ± 2.44 (IVF) (p = 0.303). There was no significant correlation between the number of embryos produced by the different techniques (SOV and IVF), indicating that cows that respond well to SOV did not respond well to IVF and vice versa (r = 0.379, p = 0.529). The total cost of each embryo produced by SOV was R$215.00 and R$410.00 for IVF. Therefore, cows that produce less than five embryos by SOV are not economically viable due their lack of response to FSH, and the use of IVF in those animals may be more effective.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Outcome and Economic Viability of Embryo Production Using IVF and SOV Techniques in the Wagyu Breed of Cattle

Facioli

Marchi

Marques

et al. 2020

Veterinary Sciences

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“…The current work demonstrated that mRNA expression of FSHR in blood prior to superstimulation predicted the ability of Holstein cows to respond better to superovulatory FSH treatment, reflected in higher number of total embryos, higher number of total transferable embryos and total grade 1 embryos. Correlations of FSHR gene with superovulation response in different breeds of cattle have been reported previously (Hirayama et al, 2019;Marson et al, 2008).…”

Section: Discussionmentioning

confidence: 90%

“…Hirayama et al. (2019) reported that number of transferable embryos collected from Japanese black cows was significantly higher in the group heterogenous for c.337C > G in FSHR. More specifically, variations of FSHR SNP may cause functional changes in G‐protein and dimensional structure of the receptor altering its response to FSH (Yang et al., 2012).…”

Section: Discussionmentioning

confidence: 99%

Transcript abundance of anti‐Mullérian hormone and follicle‐stimulating hormone receptor predicted superstimulatory response in embryo donor Holstein cows

Aziz

Abdel‐Wahab

Ibrahim

et al. 2020

Reprod Domestic Animals

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The goal was to investigate the relationship among mRNA expressions of anti-Mullérian hormone (AMH), follicle-stimulating hormone receptor (FSHR) and responses to superovulation (SO) in embryo donor dairy cows. Holstein cows (n = 19) were submitted to a standard SO protocol, with twice daily FSH treatments, and artificially inseminated. Prior to SO (Day 0), relative mRNA expressions of AMH and FSHR in blood were determined for all cows. Day 7 embryos were collected and were graded to determine superovulatory response for each donor. Results showed that relative mRNA expressions of AMH and FSHR were positively correlated (R 2 = 0.94). Relative mRNA expressions of both AMH and FSHR were positively correlated with total embryos (R 2 = 0.68 and 0.69, respectively), total transferable embryos (R 2 = 0.92 and 0.97, respectively) and total grade 1 embryos (R 2 = 0.54 and 0.59, respectively). Further, transcript abundances of AMH and FSHR positively associated with milk production of donor cows, and meanwhile, they were negatively associated with days in milk (DIM) at submission of cows to SO (p < .05) protocol. The relative mRNA expression of AMH was higher (p < .05) in donor cows <5 years of age. However, age of donor at superovulation did not influence mRNA expression of FSHR. Collectively, we infer that the mRNA expressions of AMH and FSHR prior to superovulation can predict donor cows' positive response to superovulation.

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“…These variability have been attributed to gonadotrophin preparation with differences LH:FSH activities [5][6][7][8][9] and its protocols for treatments [10,11]. In addition, antral follicle count and AMH level are contributed to these variability's [12,13]. Selection of candidate donor's cows for embryos production based on AMH level will reduce this variability [14].…”

Section: Introductionmentioning

confidence: 99%

Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration

Mohammed¹,

Darwish²,

Dohreig³

et al. 2019

AVS

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The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100µg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally subgroups ; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF 2 α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100µg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH.

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Effects of genetic background on responses to superovulation in Japanese Black cattle

Cited by 17 publications

References 14 publications

The Outcome and Economic Viability of Embryo Production Using IVF and SOV Techniques in the Wagyu Breed of Cattle

The Outcome and Economic Viability of Embryo Production Using IVF and SOV Techniques in the Wagyu Breed of Cattle

Transcript abundance of anti‐Mullérian hormone and follicle‐stimulating hormone receptor predicted superstimulatory response in embryo donor Holstein cows

Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration

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