Effects of Germination Black Soy Milk Fermented with Lactobacillus plantarum TWK10 on Anti-Oxidative and Anti-Melanogenesis

Abstract

Black soybean germination or fermentation increases active ingredient bioavailability and anti-oxidative activity. This study investigated the effects of fermented and germinated black soy milk on anti-oxidation and melanogenesis inhibition. The total phenolic content (TPC; 42.66 ± 1.65 mg gallic acid equivalent/g) and total flavonoid content (TFC; 5.43 ± 0.54 mg quercetin equivalent (QE)/g) in ethanol extracts from Lactobacillus plantarum TWK10 (TWK10)-fermented nongermination black soy milk (FNGB) were significantly (p < 0.05) higher than those in ethanol extracts from nonfermented-nongermination black soy milk (NNGB). Although the TPC of ethanol extracts from nonfermented-germination black soy milk (NGB) and fermented-germination black soy milk (FGB) were not significantly different (p > 0.05), the TFC of FGB (1.79 ± 0.08 mg QE/g) was significantly higher than that of NGB (p < 0.05). The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of ethanol extracts from NNGB, FNGB, NGB, and FGB was significantly higher than that of water extracts at 10 mg/mL (p < 0.05). Furthermore, ethanol extracts from both FNGB and FGB significantly reduced the melanin content in zebrafish embryos at 100 µg/mL (p < 0.05) without causing death, malformation or bradycardia. Overall, the antioxidant activity of black soy milk significantly increased after TWK10 fermentation; moreover, ethanol extracts from FNGB and FGB could inhibit melanogenesis, indicating their potential as whitening ingredients.

Cosmetic and Pharmaceutic Products with Selected Natural and Synthetic Substances for Melasma Treatment and Methods of Their Analysis

“…Not only extracts may be applied as a potential source of skin-whitening agents, but fermented plant materials can also serve as such. For example, Liu et al have shown that ethanol extracts from Lactobacillus plantarum TWK10 fermented-nongerminated black soy milk and fermented-germinated black soy milk could inhibit melanogenesis, indicating their potential as whitening agents in cosmetic products [150]. The extracts of Aloe vera leaf skin fermented with Lactobacillus plantarum BN41 were tested for their whitening properties.…”

Peningkatan Nilai Gizi Pada Susu Tempe Kedelai Hitam (Glycine soja sieb)

“…Studi membuktikan bahwa proses fermentasi dapat meningkatkan mutu gizi serta menurunkan senyawa anti gizi seperti tanin dan saponin (Chaturvendi & Chakraborty 2022). Selain itu, studi terbaru dari Liu et al (2022) menyimpulkan bahwa proses fermentasi pada kedelai hitam terbukti dapat meningkatkan kapasitas antioksidan secara signifikan, serta mampu menghambat proses melanogenesis. Proses fermentasi oleh Rhizopus pada kedelai hitam memicu pembentukan isoflavon yang lebih tinggi dibandingkan pada kedelai hitam tanpa fermentasi.…”

Evaluating the effect of lactic acid bacteria fermentation on quality, aroma, and metabolites of chickpea milk

“…Lactobacillus plantarum bacteria grow well in soymilk, produce some bioactive peptides and reduce soy oligosaccharides in a strain-specific manner ( 9 ). Lactobacillus plantarum fermentation improves the antioxidant activity of soymilk food products ( 10 ). The soymilk fermented with L. plantarum was also found to lower the concentration of total cholesterol, triglyceride and low-density lipoprotein cholesterol in serum ( 11 ).…”