Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells

Abstract

Ghrelin is a circulating peptide hormone, which involved in promoting feeding and regulating energy metabolism in human and rodents. Abnormal synovial hyperplasia is the most important pathologic hallmark of rheumatoid arthritis (RA), which is characterised by tumor-like expansion. Existing studies indicated that there may exist some relation between the decreased ghrelin and the abnormally proliferating synovial cells in RA. Therefore, the aim of this study is to explore the apoptotic effects of ghrelin on MH7A synovial cells in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the effects of ghrelin on the viability of MH7A cells. Terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and flow cytometry were used to test the apoptotic effects of ghrelin. At last, Western blot and real-time PCR were performed to explore the expression of caspases-8,-9, and-3 after the treatment of ghrelin. MTT experiments showed that ghrelin could inhibit viability of MH7A cells. The results of flow cytometry and TUNEL showed that ghrelin could induce apoptosis of MH7A synovial cells. Western blot showed that expression of cleaved-caspases-8,-9, and-3 were increased in ghrelin stimulation group compared with the control group, while expression of pro-caspases-8,-9, and-3 had no significant difference. In mRNA levels, ghrelin can decrease pro-caspases-8,-9, and-3 mRNA expression, which confirmed the results of protein levels. Then these apoptotic effects were significantly reversed by [D-Lys 3 ] GHRP-6 (ghrelin receptor antagonist). This study found that ghrelin can induce apoptosis of MH7A cells through caspase signaling pathways.

Ghrelin is associated with anti‐mullerian hormone levels in Chinese systemic lupus erythematosus

“…No obvious association was found between ghrelin and the SLEDAI scores in obese and non‐obese SLE patients. Ghrelin levels were significant lower in obese SLE patients with arthritis than those without, which was in accorded with some previously researches that ghrelin was decreased in rheumatoid arthritis and osteoarthritis 29–31 . A previous study found that ghrelin can reduce the expression of TNF‐α 30 .…”

“…Ghrelin levels were significant lower in obese SLE patients with arthritis than those without, which was in accorded with some previously researches that ghrelin was decreased in rheumatoid arthritis and osteoarthritis. [29][30][31] A previous study found that ghrelin can reduce the expression of TNF-α. 30 Besides, ghrelin can decrease IL-1β induced MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 increasing in chondrocytes.…”

Anti-inflammatory effects of Jingshu Keli capsule and its components on human synoviocyte MH7A cells

“…The human synovial cell line MH7A was purchased from Riken Cell Bank (Ibaraki, Japan) and cultured in RPMI-1640 medium (Hyclone, USA) with 10% fetal bovine serum (FBS, Thermo, USA) and 1% penicillin/streptomycin (P/S, Hyclone, USA) at 37 °C in 5% CO 2 humidified atmosphere [ 23 ]. Primary synovial cells from a rheumatoid arthritis patient were kindly provided by Dr. Qingwen Wang from Peking University Shenzhen Hospital, Shenzhen, China.…”

Mechanism of Mir-218-5P in Autophagy, Apoptosis and Oxidative Stress in Rheumatoid Arthritis Synovial Fibroblasts is Mediated by Klf9 and Jak/Stat3 Pathways

“…Recent research suggests that as the main synovial cell type in synovial tissue, rheumatoid arthritis synovial fibroblast (RASF) is involved in maintaining the homeostasis of the synovial environment 7. Studies have reported that the transformation of biological characteristics of RASFs in the progression of RA is an early pathological change 8 9. Research based on RASFs has become a direction for RA treatment.…”