Effects of ginger (Zingiber officinale) supplementation on testicular histology, semen characteristic, blood plasma parameters and reproductive performance in aged broiler breeder roosters

Abstract

Higher long-chain polyunsaturated fatty acids contents in roosters' sperm plasma membrane along with age-related decrease in antioxidant defense make the spermatozoa very susceptible to lipid peroxidation. Ginger root contains abundant amounts of gingerol, shogaols, gingerdiol and other active compounds, which known as antioxidant compounds to enhance semen quality. The goal of the study was to evaluate the effect of dietary supplementation of ginger root on semen quality, blood chemistry, immune response, testicular histology and reproductive performance of Ross-308 breeder roosters from 47 to 60 weeks of age. The feeding of ginger root resulted in an increase in parameters related to sperm forward motility and seminal total antioxidant capacity (TAC), and following there was a tendency to increase and decrease in seminal superoxide dismutase activity and malondialdehyde concentration, respectively; however, sperm concentration was not affected. There was an increase and tendency to increase in blood total protein and TAC in the supplemented group respectively.The roosters fed ginger supplemented diet had a higher spermiation index; and following there was tendency to increase seminal tubes spermatozoids number (p = 0.056) and repopulation index (p = 0.058). Despite the improved seminal antioxidant status and a tendency to lower embryonic mortality in the gingerreceived group, the fertility and hatchability rate of roosters were statistically insignificant. Supplementations of ginger root in ageing rooster's diet had a beneficial effect on sperm motility, seminal antioxidant status and testicular spermiation index.

Gallic acid supplementation partially ameliorates reproductive aging in rooster breeders by improving semen quality, sperm kinetics, hormones, and antioxidant status

“…Authaida et al (2024) , found that Thai ginger (Kaempferia parviflora) extract improved sperm production, semen preservation, and fertility in Thai native chickens under heat stress conditions. Similarly, Nemati et al (2023) , demonstrated that ginger (Zingiber officinale) supplementation enhanced testicular histology, semen characteristics, blood plasma parameters, and reproductive performance in aged broiler breeder roosters. Their study showed that the antioxidant compounds like gingerol, shogaols and gingerdiol in ginger root helped enhance semen quality by reducing lipid peroxidation in the sperm plasma membrane of aged roosters.…”

Feeding pomegranate pulp to Ghezel lambs for enhanced productivity and meat quality

“…Samples were held on ice and within 2 h of collection they were centrifuged at 1300 × g for 15 min to separate the serum from the plasma, the former frozen at −20 °C until analysis. Serum concentrations of urea (using enzymatic Urease-GLDH method; mg/dL), triglycerides (Catalogue number: 117,500; mg/dL), total cholesterol (Catalogue number: 128,500; mg/dL), low density lipoprotein cholesterol (using enzymatic methods; mg/dL), high density lipoprotein cholesterol (mg/dL), aspartate amino transferase (Catalogue number: 118,400; U/L), alanine amino transferase (U/L), alkaline phosphatase (Biochrom WPA Biowave S2100 Diode Array Spectrophotometer; U/L), and lactate dehydrogenase were quantified as per the manufacturer's instructions of the listed laboratory kits (Pars Azmoon Co., IRN) and a benchtop autoanalyzer (ALCYON-300, Abbott, USA) ( Nemati et al, 2023 ).…”