Effects of Ginseng Berry Water Extract on the Polysaccharide Hydrolysis of Extracellular Enzymes and Intracellular PTP1B and AKT1

Abstract

Ginseng has been known to be highly effective for health as a traditional medicinal herb. Ginseng berry, or fruit of ginseng, contains ginsenoside, saponin, polyphenol, polyacetylene, alkaloid, etc. as the main compounds as does ginseng. The aim of this study is to evaluate any effect of ginseng berry water extract (GBE) on diabetic-associated molecules, such as enzymes, which are responsible for the glucose entry of the cells and the insulin receptor signaling molecules using HepG2 cells. Therefore, two enzymes, α-amylase and α-glucosidase, were selected and assayed for their activities in the presence of GBE in vitro. These two enzymes are responsible for producing glucose from dietary starch.Protein-tyrosine phosphatase 1B (PTP1B) and Akt1 are key proteins in the insulin receptor signaling pathway. These two intracellular signaling molecules were investigated for their expression levels in HepG2 cells after insulin and GBE treatment. GBE, at concentrations up to 1,000 μg/ml, did not exert any inhibitory effect on α-amylase and α-glucosidase. It was observed that the expression level of PTP1B was increased by insulin and the 25 μg/ml GBE treatment enhanced the PTP1B level. However, GBE at a concentration of 200 μg/ml reduced the expression level of PTP1B. In the case of Akt1, the Akt1 level by insulin was decreased by GBE treatment. These data suggest that the water extracts of ginseng berry have an influence on intracellular signaling by insulin.

Effect of Submerged Culture of Ceriporia lacerata Mycelium on Insulin Signaling Pathway in 3T3-L1 Cell

“…세계적으로 당뇨병 치료에 효과 가 있을 것으로 보고된 천연물은 전세계적으로 약 400여 종 이상으로 추정되며 [12,31] 국내에 지금까지 소개된 혈당 강하 활성을 가지는 천연물에는 인삼열매 [14], 알로에 [9], 담쟁이덩 굴 [5] 등 다양한 소재가 있고 큰느타리버섯 [11], 표고버섯 [25], 잎새버섯 [22] Western blot으로 3T3-L1 세포에서의 단백질을 측정한 결과를 단백질에 대한 인산화 단백질의 상대적인 수치로 나타내었다 (Fig. 2, Fig.…”