Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

Abstract

Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parame¬ters of all study groups were checked after equilibration and freezing. Results: After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different (p < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher (p < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher (p < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased (p < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450. Conclusion: According to the findings, the supplementation of 150 mm trehalose in the TCEF dilu¬ent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI.

Various Saccharides Addition of Extender Ram Efficiency to Improve Cryopreservation in Semen Etawah Crossbreed Bucks with Seminal Plasma Replacement

“…The result also showed that utilizing disaccharides could maintain motility better than other saccharides this finding is also similar to previous research which stated lactose/disaccharide is the best saccharide type to preserve frozen semen (Lapwood and Martin, 1966), our result is in agreement with findings in boar that comparing monosaccharides and disaccharides on sperm quality of boar sperm cryopreserved with an egg yolk based extender (Gómez-Fernández et al, 2012) this occurs due to disaccharides more being able to cross the cell membrane than the trisaccharides also less easily converted into energy than monosaccharides thereby better able to maintain the membrane integrity of cells than other saccharides (Kamal et al, 2023). Disaccharides could prevent the formation of ice crystals by forming hydrogen bonds between molecules in the lipid bilayer of cryopreserved semen (Uchida et al, 2007).…”

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

“…Several extenders supplemented with various sugars have been used for the preservation of mammalian semen, including glucose (De los Reyes et al., 2002), lactose (Salamon & Maxwell, 2000), fructose (Ponglowhapan et al., 2004) and raffinose (Salamon & Maxwell, 2000). Furthermore, recently, sucrose and trehalose have been the most commonly used disaccharides for protecting semen mammals during the freezing process, such as pig (Pezo et al., 2020), ram (Jia et al., 2021) and goat (Kamal et al., 2023) (Table 3).…”

Efecto de diferentes crioprotectores sobre la criopreservación del semen de cerdo Pelón Mexicano

“…No obstante, se han evaluado diversos azúcares como crioprotectores no penetrantes, entre los cuales se incluyen: glucosa, fructosa, lactosa y trehalosa (De mercado et al, 2010;Athurupana et al, 2015;Cantanhêde et al, 2018;Pezo et al, 2020;Kamal et al, 2023) donde, los que han dado mejores resultados en cerdos comerciales postdescongelación son la lactosa y la trehalosa, sin embargo, se desconocen los efectos de diferentes diluyentes sobre la calidad seminal del cerdo pelón mexicano . Por lo que, el objetivo fue evaluar diferentes azúcares en el diluyente de congelación para semen de cerdo pelón mexicano y su efecto sobre la calidad seminal a la descongelación.…”