Effects of Glucosinolates and Flavonoids on Colonization of the Roots of
            <i>Brassica napus</i>
            by
            <i>Azorhizobium caulinodans</i>
            ORS571

O’Callaghan, K. J.; Stone, Philip J.; Hu, Xiaojia; Griffiths, D. W.; Davey, M. R.; Cocking, E. C.

doi:10.1128/aem.66.5.2185-2191.2000

Cited by 55 publications

(26 citation statements)

References 35 publications

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“…Changes linked to glucosinolates and derivatives in Alphaproteobacteria community, particularly Rhizobiaceae, were less explored in the literature. Ground root tissue of Brassica was toxic to Bradyrhizobium pure strains (Trinick and Hadobas, 1995) and in vitro colonization of Brassica napus roots by the Alphaproteobacteria Azorhizobium caulinodans was affected by plant glucosinolate content (O'Callaghan et al, 2000). In our study, we show different susceptibilities of spontaneous Rhizobiaceae populations exposed to Brassicaceae roots.…”

Section: Discussionmentioning

confidence: 61%

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

et al. 2009

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A specificity of Brassicaceous plants is the production of sulphur secondary metabolites called glucosinolates that can be hydrolysed into glucose and biocidal products. Among them, isothiocyanates are toxic to a wide range of microorganisms and particularly soil-borne pathogens. The aim of this study was to investigate the role of glucosinolates and their breakdown products as a factor of selection on rhizosphere microbial community associated with living Brassicaceae. We used a DNA-stable isotope probing approach to focus on the active microbial populations involved in root exudates degradation in rhizosphere. A transgenic Arabidopsis thaliana line producing an exogenous glucosinolate and the associated wild-type plant associated were grown under an enriched 13 CO 2 atmosphere in natural soil. DNA from the rhizospheric soil was separated by density gradient centrifugation. Bacterial (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Acidobacteria), Archaea and fungal community structures were analysed by DGGE fingerprints of amplified 16S and 18S rRNA gene sequences. Specific populations were characterized by sequencing DGGE fragments. Roots of the transgenic plant line presented an altered profile of glucosinolates and other minor additional modifications. These modifications significantly influenced microbial community on roots and active populations in the rhizosphere. Alphaproteobacteria, particularly Rhizobiaceae, and fungal communities were mainly impacted by these Brassicaceous metabolites, in both structure and composition. Our results showed that even a minor modification in plant root could have important repercussions for soil microbial communities.

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Section: Discussionmentioning

confidence: 61%

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

et al. 2009

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“…The biological effects of GSLs and GHPs have been known since the early 1990s, when several authors investigated their effects on the growth and development of bacteria (19,23), insects (24)(25)(26), fungi (27,28), and nematodes (29,30), and our knowledge about the deterrent or attractant effects of the main glucosinolates on different pests (generalists and specialists) and parasitoids is well documented. Other authors have tested the effects of GHPs and GSLs on soil pathogens by incorporating Brassica residues into soil or by testing their effect by using in vitro assays.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

Sotelo

Márquez

Soengas

et al. 2015

Appl Environ Microbiol

113

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bGlucosinolates (GSLs) are secondary metabolites found in Brassica vegetables that confer on them resistance against pests and diseases. Both GSLs and glucosinolate hydrolysis products (GHPs) have shown positive effects in reducing soil pathogens. Information about their in vitro biocide effects is scarce, but previous studies have shown sinigrin GSLs and their associated allyl isothiocyanate (AITC) to be soil biocides. The objective of this work was to evaluate the biocide effects of 17 GSLs and GHPs and of leaf methanolic extracts of different GSL-enriched Brassica crops on suppressing in vitro growth of two bacterial (Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. maculicola) and two fungal (Alternaria brassicae and Sclerotinia scletoriorum) Brassica pathogens. GSLs, GHPs, and methanolic leaf extracts inhibited the development of the pathogens tested compared to the control, and the effect was dose dependent. Furthermore, the biocide effects of the different compounds studied were dependent on the species and race of the pathogen. These results indicate that GSLs and their GHPs, as well as extracts of different Brassica species, have potential to inhibit pathogen growth and offer new opportunities to study the use of Brassica crops in biofumigation for the control of multiple diseases.T he genus Brassica belongs to the family Brassicaceae (also known as Cruciferae); economically speaking, it is the most important genus within the tribe Brassicaceae, containing 37 different species. Brassica vegetables are of great economic importance throughout the world. Currently, Brassica crops, together with cereals, represent the basis of world food supplies. In 2007, Brassica vegetables were cultivated in more than 142 countries around the world, and they occupied more than 4.1 million ha (1).The productivity and quality of important Brassica crops (e.g., cabbage, oilseed rape, cauliflower, Brussels sprouts, kale, and broccoli) are seriously affected by several diseases, which result in substantial economic losses (2). Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Pammel), is considered to be one of the most important pathogens affecting Brassica vegetables worldwide (3). There are nine races of Xanthomonas campestris pv. campestris: races 1 to 6 were described by Vicente et al. (4) and races 7 to 9 by Fargier and Manceau (5). It is recognized that races 1 and 4 are the most virulent and widespread, accounting for most of the black rot recorded around the world (4).Bacterial leaf spot, caused by Pseudomonas syringae pv. maculicola (McCulloch) (6), is very significant on cauliflower but also occurs on broccoli, Brussels sprouts, and other brassicas. P. syringae pv. maculicola may also cause leaf blight on the oilseed species Brassica juncea and Brassica rapa (3).Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a widespread fungal disease in temperate areas of the world and also occurs in warmer and drier areas during the winter months or the rain...

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“…Only a few reports investigate the effect of PGPB on glucosinolate synthesis (O'Callaghan et al 2000;Bending and Lincoln 2000). We therefore measured glucosinolates in leaf and tuber material of radish plants that were inoculated with K. radicincitans compared to non-inoculated control plants at the time of harvest.…”

Section: Glucosinolate Profile In K Radicincitans-inoculated Radishmentioning

confidence: 99%

K. radicincitans, a beneficial bacteria that promotes radish growth under field conditions

Berger

Wiesner

Brock

et al. 2015

Agron. Sustain. Dev.

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Conserving arable soils is a major challenge in agronomy. Using beneficial native microorganisms such as plant growth-promoting bacteria contributes to sustainability in agricultural systems and helps maintain stable yields and product quality. However, the growth-promoting effects of candidate strains observed in pot trials often disappear under field conditions. Therefore, we studied here the bacterial colonization of Kosakonia radicincitans, a known plant growthpromoting bacteria, and the effect of K. radicincitans on radish. We measured plant growth and glucosinolate profile when plants were grown under glasshouse and field conditions. K. radicincitans cells were applied onto seeds or plants when two leaves had emerged. Our results show an increase of up to 25 % of leaf and tuber weights of inoculated radish plants under glasshouse and field conditions, compared to non-inoculated plants. Glucosinolate contents remained unchanged, except when leaves were sprayed with K. radicincitans. We also found that K. radicincitans suppressed aromatic 2-phenylethyl glucosinolates in leaves. To sum up, we demonstrate for the first time the capability of K. radicincitans to persist and promote plant growth in a member of the Brassicaceae family under field conditions. Therefore, K. radicincitans is a promising candidate for further processing as a growth-promoting product in sustainable agriculture.

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Effects of Glucosinolates and Flavonoids on Colonization of the Roots of Brassica napus by Azorhizobium caulinodans ORS571

Cited by 55 publications

References 35 publications

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

K. radicincitans, a beneficial bacteria that promotes radish growth under field conditions

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