Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum

Li, He; Qiao, Jinjin; Shangguan, Jiaolei; Zhu, Jing

doi:10.1007/s00253-023-12417-3

Cited by 6 publications

(3 citation statements)

References 71 publications

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“…In G. lucidum, GCN4 enhanced the expression of asns, which is the typical target gene of GCN4 in mammals [47]. In addition, GOT coordinates carbon and nitrogen metabolism in organisms [48] and affects the intracellular glutamate content [49], which is the key metabolite in the intracellular amino acid biosynthesis [50]. In our study, genes of transaminases (got1 and got2) were regulated by GCN4.…”

Section: Discussionmentioning

confidence: 54%

A transcription factor GCN4 contributes to maintaining intracellular amino acid contents under the nitrogen limiting conditions in mushroom Ganoderma lucidum

Lian

Qiao

Guo

et al. 2023

Preprint

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Background Edible mushrooms, with their delightful taste and abundant protein and amino acids required by human, can be used as substitutes of meat or plant proteins in a variety of foods and beverages. Nitrogen limiting conditions promote the development of fruiting bodies in mushrooms, but are not conducive to the accumulation of amino acids. Previous study has revealed that GCN4 has a pivotal role in response to nitrogen limiting conditions in Ganoderma lucidum. But its influence on amino acid contents is nearly unknown. Results In this study, we found that the amino acid contents reached 120.51 mg per gram of mycelia in the WT strain under the 60 mM asparagine condition, but decreased by 62.96% under the 3 mM asparagine condition. Second, silencing of gcn4 resulted in a 54.2% decrease of amino acid contents under the 60 mM asparagine condition, especially for the essential and monosodium glutamate-like taste amino acids. However, these were more pronounced under the 3 mM asparagine condition. Third, silencing of gcn4 markedly inhibited the expression of amino acid biosynthesis and transporting genes. In addition, GCN4 enhanced the tricarboxylic acid cycle (TCA) and glycolytic pathway, and inhibited the activity of target of rapamycin complex 1 (TORC1), thus being beneficial for maintaining amino acid homeostasis. Conclusion This study confirmed that GCN4 contributed to maintaining the amino acid contents in mushrooms under the low concentration of nitrogen. In conclusion, our study provides a research basis for GCN4 to regulate amino acid synthesis and improve nutrition of edible mushrooms.

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Section: Discussionmentioning

confidence: 54%

A transcription factor GCN4 contributes to maintaining intracellular amino acid contents under the nitrogen limiting conditions in mushroom Ganoderma lucidum

Lian

Qiao

Guo

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In G. lucidum , GCN4 enhanced the expression of asns , which is the typical target gene of GCN4 in mammals [ 41 ]. In addition, GOT coordinates carbon and nitrogen metabolism in organisms [ 42 ] and affects the intracellular glutamate content [ 43 ], which is the key metabolite in the intracellular amino acid biosynthesis [ 44 ]. In our study, transaminase genes ( got1 and got2 ) were regulated by GCN4.…”

Section: Discussionmentioning

confidence: 99%

The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Lian,

Qiao,

Guo

et al. 2023

Microb Cell Fact

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Background Edible mushrooms are delicious in flavour and rich in high-quality protein and amino acids required by humans. A transcription factor, general control nonderepressible 4 (GCN4), can regulate the expression of genes involved in amino acid metabolism in yeast and mammals. A previous study revealed that GCN4 plays a pivotal role in nitrogen utilization and growth in Ganoderma lucidum. However, its regulation is nearly unknown in mushrooms. Results In this study, we found that the amino acid contents reached 120.51 mg per gram of mycelia in the WT strain under 60 mM asparagine (Asn) conditions, but decreased by 62.96% under 3 mM Asn conditions. Second, silencing of gcn4 resulted in a 54.2% decrease in amino acid contents under 60 mM Asn, especially for the essential and monosodium glutamate-like flavour amino acids. However, these effects were more pronounced under 3 mM Asn. Third, silencing of gcn4 markedly inhibited the expression of amino acid biosynthesis and transport genes. In addition, GCN4 enhanced the tricarboxylic acid cycle (TCA) and glycolytic pathway and inhibited the activity of target of rapamycin complex 1 (TORC1), thus being beneficial for maintaining amino acid homeostasis. Conclusion This study confirmed that GCN4 contributes to maintaining the amino acid contents in mushrooms under low concentrations of nitrogen. In conclusion, our study provides a research basis for GCN4 to regulate amino acid synthesis and improve the nutrient contents of edible mushrooms.

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“…Moreover, our result showed that the expression of gene encoding pyridoxal 10.3389/fnut.2024.1375386 phosphate-dependent transferase was up-regulated, which was a member of the aspartate aminotransferase (AAT) family. Previous studies showed that the gene encoding glutamic oxaloacetic aminotransferase (GOT) in Ganoderma lucidum was involved in the alterations of amino acid content, metabolism of carbohydrate and protein, and the production of ROS (59), while the degradation activity of Serrella marcescens was closely related to the upregulation of succinyl-diaminopimelate transaminase (60). These studies indicated that the oxidative degradation of truffle fruiting bodies was closely related to the up-regulation of genes encoding the enzymes in the AAT family.…”

Section: Effect Of Cold Storage Time On Expression Of Genes Associate...mentioning

confidence: 99%

Transcriptome analysis reveals the effect of cold storage time on the expression of genes related to oxidative metabolism in Chinese black truffle

Zhang,

Yang,

Yao

et al. 2024

Front. Nutr.

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Chinese black truffle (Tuber indicum) is a hypogenous fungus of great value due to its distinctive aroma. In this study, both transcriptome and physicochemical analyses were performed to investigate the changes of nutrients and gene expression in truffle fruiting bodies during cold storage. The results of physicochemical analysis revealed the active metabolism of fruiting bodies in cold storage, showing the decreased contents of protein and soluble sugar, the variations in both polyphenol oxidase activity and total phenol content, and the detrimental effect of reactive oxygen species production caused by heavy metals (cadmium and lead) in truffles. Transcriptome analysis identified a total of 139,489 unigenes. Down-regulated expression of genes encoding the catalase-like domain-containing protein (katE), glutaredoxin protein (GRX), a copper/zinc superoxide dismutase (Sod_Cu), and aspartate aminotransferase (AAT) affected the degradation metabolism of intracellular oxides. Ribulose-5-phosphate-3-epimerase (RPE) was a key enzyme in response to oxidative stress in truffle cells through the pentose phosphate pathway (PPP). A total of 51,612 simple sequence repeats were identified, providing valuable resources for further genetic diversity analysis, molecular breeding, and genetic map-ping in T. indicum. Transcription factors GAL4 and SUF4-like protein were involved in glucose metabolism and histone methylation processes, respectively. Our study provided a fundamental characterization of the physicochemical and molecular variations in T. indicum during the cold storage at 4°C, providing strong experimental evidence to support the improvement of storage quality of T. indicum.

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Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum

Cited by 6 publications

References 71 publications

A transcription factor GCN4 contributes to maintaining intracellular amino acid contents under the nitrogen limiting conditions in mushroom Ganoderma lucidum

A transcription factor GCN4 contributes to maintaining intracellular amino acid contents under the nitrogen limiting conditions in mushroom Ganoderma lucidum

The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Transcriptome analysis reveals the effect of cold storage time on the expression of genes related to oxidative metabolism in Chinese black truffle

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