Effects of Glutamine and Taurine on Preimplantation Development and Cleavage of Mouse Embryos in Vitro1

Devreker, Fabienne; Hardy, Kate

doi:10.1095/biolreprod57.4.921

Cited by 76 publications

(45 citation statements)

References 48 publications

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“…Indeed, we think that all the groups are in same stage of differentiation and the difference in cell numbers are secondary to increase incidence of apoptosis or decrease in cell proliferation, as confirmed by our microarray data. Other authors (Devreker & Hardy 1997) have documented a significant change in cell number, mitotic, and apoptotic index in blastocysts cultured in different media.…”

Section: Discussionmentioning

confidence: 87%

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos

Giritharan¹,

Talbi²,

Donjacour³

et al. 2007

Reproduction

144

127

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In vitro culture (IVC) of preimplantation mouse embryos is associated with changes in gene expression. It is however, not known if the method of fertilization affects the global pattern of gene expression. We compared gene expression and development of mouse blastocysts produced by in vitro fertilization (IVF) versus blastocysts fertilized in vivo and cultured in vitro from the zygote stage (IVC) versus control blastocysts flushed out of the uterus on post coital day 3.5. The global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. It appears that each method of fertilization has a unique pattern of gene expression and development. Embryos cultured in vitro had a reduction in the number of trophoblastic cells (IVF 33.5 cells, IVC 39.9 cells, and 49.6 cells in the in vivo group) and, to a lesser degree, of inner cell mass cells (12.8, 11.7, and 13.8 respectively). The inner cell mass nuclei were larger after culture in vitro (140 mm 2 , 113 mm 2 , and 86 mm 2 respectively). Although a high number of genes (1912) was statistically different in the IVF cohort when compared with the in vivo control embryos, the magnitude of the changes in gene expression were low and only a minority of genes (29 genes) was changed more than fourfold. Surprisingly, IVF embryos were different from IVC embryos (3058 genes were statistically different, but only three changed more than fourfold). Proliferation, apoptosis, and morphogenetic pathways are the most common pathways altered after IVC. Overall, IVF and embryo culture have a profound effect on gene expression pattern and phenotype of mouse preimplantation embryos.

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Section: Discussionmentioning

confidence: 87%

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos

Giritharan¹,

Talbi²,

Donjacour³

et al. 2007

Reproduction

144

127

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“…Sperm (1-2!10 6 ) were added to the oocytes held in a 0.5 ml drop of T6 medium and incubated for 4-5 h at 37 8C in 5% CO 2 to allow fertilisation to occur. Oocytes were then transferred to 200 ml drops of KSOM medium (Devreker & Hardy 1997) overlaid with silicon fluid and incubated at 37 8C in 5% CO 2 to allow development to proceed. The following day 2-cell embryos were scored as a measure of fertilisation and then transferred to smaller drops (2 ml per embryo) of KSOM and left in the incubator to develop further.…”

Section: In Vitro Fertilisation (Ivf)mentioning

confidence: 99%

A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice

Paul¹,

Murray²,

Spears³

et al. 2008

Reproduction

237

168

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Infertility represents a major clinical problem and 50% of cases are attributable to the male partner. Testicular function is temperature dependent, and in both man and mouse the position of the testes in the scrotum ensures that they are kept at between 2 and 8 8C below core body temperature. We used a mouse model to investigate the impact of a single, transient, mild, scrotal heat stress (38, 40 or 42 8C for 30 min) on testicular function, sperm DNA integrity and embryo survival. We detected temperature-dependent changes in testicular architecture, number of apoptotic cells and a significant reduction in testis weight 7 and 14 days after heat stress at 42 8C. We report for the first time that DNA strand breaks (g-H2AX-positive foci) were present in spermatocytes recovered from testes subjected to 40 or 42 8C. Fertility of heat-stressed males was tested 23-28 d after treatment (sperm at this time would have been spermatocytes at time of heating). Paternal heat stress at 42 8C resulted in reduced pregnancy rate, placental weight and litter size; pregnancies from the 40 8C group had increased resorptions at e14.5. Abnormalities in embryonic development were detected at e3.5 and in vitro fertilisation with sperm recovered 16 h or 23 d after scrotal stress at 42 8C revealed a block in development between the 4-cell and blastocyst stages. This study has provided evidence of temperature-dependent effects on germ cell DNA integrity and highlighted the importance of an intact paternal genome for normal embryo development. Reproduction (2008) 136 73-84

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“…Sperm (1 to 2 ϫ 10 6 ) were added to 30 to 40 oocytes held in a 0.5-ml drop of T6 medium and incubated for 4 to 5 hours at 37°C in 5% CO 2 to allow fertilization to occur. Oocytes were then transferred to 200-l drops of KSOM medium, 32 overlaid with silicone fluid, and incubated at 37°C in 5% CO 2 to allow development to proceed. The following day two-cell embryos were scored as a measure of successful fertilization and transferred to smaller drops (2 l of medium per embryo) of KSOM and left in the incubator to develop further.…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

Paisley

Banks

Selfridge

et al. 2004

The American Journal of Pathology

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The prion protein (PrP) and Doppel (Dpl) have many structural and biochemical properties in common, leading to the suggestion that the lack of an obvious phenotype in PrP-deficient mice maybe because of compensation by Dpl. To test this hypothesis and also investigate the function of Dpl we have generated Prnd ؊/؊ and Prnp ؊/؊ /Prnd ؊/؊ mouse lines. Both develop normally and display an identical male sterility phenotype that differs from that reported for another Prnd ؊/؊ mouse line. Sperm from both our mutant lines were present at normal concentrations, had normal motility, and no morphological abnormalities. Despite only rarely fertilizing oocytes in vivo, because of an inability to perform the acrosome reaction, mutant sperm were capable of fertilization in vitro, albeit at reduced rates compared to wild type. Elevated levels of oxidative DNA damage were found in both types of mutant sperm and resulting embryos failed at an early stage. Therefore we found no evidence that Dpl compensates for the loss of PrP function in mutant mouse lines, but it does have an important anti-oxidant function necessary for sperm integrity and male fertility.

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Effects of Glutamine and Taurine on Preimplantation Development and Cleavage of Mouse Embryos in Vitro1

Cited by 76 publications

References 48 publications

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos

A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice

Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

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