Effects of Glutamine on Growth Performance, Intestinal Morphology and Intestinal Barrier Function of Broilers

Wu, Qiu Jue; Liu, Z. H.; Jiao, C; Cheng, BY; Zhu, Dengya; Ma, Yan; Wang, Y. Q.; F., W.; Wang, Y.

doi:10.18805/ijar.b-1207

Cited by 4 publications

(6 citation statements)

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“…In our present study, S. Enteritidis infection led to a significant decrease in villus height and a significant increase in crypt depth such that the VH:CD was reduced in the broilers infected with Salmonella. Similarly, previous reports showed that the VH:CD in the small intestine significantly reduced upon Salmonella infection (Andrade et al, 2013; Jazi et al, 2018;Wu et al, 2020). This outcome may be the result of Salmonella damaging the intestinal mucosa, affecting the absorption of nutrients, weakening the body's resistance, and then aggravating the intestinal mucosa.…”

Section: Discussionsupporting

confidence: 64%

Effects of Glutamine on the Mucosal Structure and Immune Cells in the Intestines of Broiler Chickens Challenged with Salmonella Enteritidis

Liu

Jiao

et al. 2020

Braz. J. Poult. Sci.

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The aim of this study was to investigate the effects of glutamine (Gln) on the intestinal mucosal structure and immune cells of broilers infected with Salmonella Enteritidis. 160 1-d-old commercial Arbor Acres (AA) broilers were randomly selected to receive one of four treatments, each of which had 5 replicates. Each replicate consisted of 8 chicks subjected to a 21-d feeding trial. Group I served as the unchallenged (CON). All birds in groups II (SCC) -IV were challenged with 2.0 × 10 4 CFU/mL of S. Enteritidis. The birds in groups III and IV were treated with 0.5% and 1.0% Gln. The results showed that S. Enteritidis infection led to a decrease in the relative length and weight, villus height:crypt depth (VH:CD) of the jejunum and ileum, the number of intraepithelial lymphocyte cells, and number of goblet cells and an increase in the number of mast goblet cells compared with the measurements of these parameters in the CON group (p<0.05). In addition, the Gln groups had increased relative length and weight, VH:CD of the jejunum and ileum, numbers of intraepithelial lymphocyte cells, and numbers of goblet cells and decreased crypt depth in the jejunum and ileum and numbers of mast goblet cells compared with the measurements of these parameters in the SCC group (p<0.05). It was concluded that Gln added to broiler diets can effectively alleviate the intestinal mucosal damage caused by S. Enteritidis infection and improve its normal defense barrier function.

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Section: Discussionsupporting

confidence: 64%

Effects of Glutamine on the Mucosal Structure and Immune Cells in the Intestines of Broiler Chickens Challenged with Salmonella Enteritidis

Liu

Jiao

et al. 2020

Braz. J. Poult. Sci.

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“…However, it did lead to an increase ( p < 0.05) in the feed/gain ratio of the broilers from d 22 to d 42. These data have been published in our previous paper [ 33 ].…”

Section: Resultsmentioning

confidence: 84%

“…The small intestine is a vital organ in the digestive system, where most nutrient absorption occurs. Previous studies have demonstrated that Zn deficiency had a negative impact on the villi structure of the small intestine of broilers [ 1 , 33 ]. The destruction of the intestinal physical barrier can cause nutrient absorption deficiency and thus affect the growth performance of animals.…”

Section: Discussionmentioning

confidence: 99%

“…Intestinal morphology indexes, such as VH, VSA, CD, and the VH/CD ratio, are commonly used indices to assess intestinal development and barrier function [ 34 ]. A previous study in our laboratory indicated that Zn deficiency decreased the jejunal VH and VSA and increased the duodenal and jejunal CD, but it decreased the duodenal, jejunal, and ileal VH/CD ratios of the broilers on d 42 [ 33 ]. In the present research, we found that Zn deficiency increased the duodenal and jejunal CD and decreased the duodenal, jejunal, and ileal VH/CD ratios of the broilers on d 28, while Zn deficiency increased the ideal CD and decreased the jejunal VH, jejunal and ileal VSA, and VH/CD of the broilers on d 35.…”

Section: Discussionmentioning

confidence: 99%

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Dietary Zn Deficiency Inhibits Cell Proliferation via the GPR39-Mediated Suppression of the PI3K/AKT/mTOR Signaling Pathway in the Jejunum of Broilers

Hu,

Yang,

Zhang

et al. 2024

Animals

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A prior investigation revealed that a lack of Zinc (Zn) could hinder intestinal cell proliferation in broiler chickens; however, the mechanisms responsible for this effect remain unclear. We aimed to investigate the possible mechanisms of dietary Zn deficiency in inhibiting the jejunal cell proliferation of broilers. For this study, a total of 112 chickens (21 days old) were randomly divided into two treatments (seven replicate cages per treatment, eight chickens per replicate cage): the control group (CON) and the Zn deficiency group. The duration of feeding was 21 d. Chickens in the control group were provided with a basal diet containing an extra addition of 40 mg Zn/kg in the form of Zn sulfate, whereas chickens in the Zn deficiency group were given the basal diet with no Zn supplementation. The results indicated that, in comparison to the CON, Zn deficiency increased (p < 0.05) the duodenal and jejunal crypt depth (CD) of broilers on d 28 and jejunal and ileal CD on d 35, and decreased (p < 0.05) the duodenal, jejunal, and ileal villus height/crypt depth (VH/CD) on d 28 and the jejunal VH, jejunal and ileal villus surface area, and VH/CD on d 35. Furthermore, Zn deficiency decreased (p < 0.0001) the number of proliferating cell nuclear antigen-positive cells and downregulated (p < 0.01) the mRNA or protein expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated PI3K, phosphorylated serine–threonine kinase (AKT), phosphorylated mechanistic target of rapamycin (mTOR), G protein-coupled receptor 39 (GPR39), and extracellular-regulated protein kinase, but upregulated (p < 0.05) the mRNA or protein expression levels of P38 mitogen-activated protein kinase, c-jun N-terminal kinase (JNK) 1 and JNK2, and phosphorylated protein kinase C in the jejunum of the broilers on d 42. It was concluded that dietary Zn deficiency inhibited cell proliferation possibly via the GPR39-mediated suppression of the PI3K/AKT/mTOR signaling pathway in the jejunum of broilers.

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“…Intestinal epithelial injury stress is one of the most common stresses in poultry feeding. Intestinal epithelial injury generally leads to the apoptosis of epithelium cell, the destruction of barrier function etc., which resulting in a series of reactions such as swelling, bleeding and necrosis of intestinal epithelial cells, causing the bird unable to digest the feed, resulting in significant economic losses to the breeding industry (Wu et al, 2019;Yang et al, 2021). The reasons of intestinal epithelial injury are often complex and diverse and the repair mechanism of intestinal epithelial injury has always been the focus and hotspot of research.…”

Section: Introductionmentioning

confidence: 99%

Effects of Methylsulfonylmethane and Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor on Cell Proliferation, Cell Cycle and Anti-Inflammatory Effect in Chicken Intestinal Epithelial Cells with Lipopolysaccharide Challenge

Yang¹,

Cheng²,

Li³

et al. 2023

IJAR

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Background: Intestinal epithelial injury stress is one of the most common stresses in the contemporary poultry breeding. This study aims to evaluate the protective effects of Methylsulfonylmethane (MSM) and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI, erlotinib) on injury stress induced by lipopolysaccharides (LPS) in chicken intestinal epithelial cells (IECs). Methods: Cell Counting Kit-8 (CCK-8) and EdU assays were used to evaluate cell viability and proliferation. The cell cycle was analyzed by flow cytometry method. The mRNA transcript and protein expression levels of EGFR were detected by RT-qPCR and Western blotting (WB). The levels of pro-inflammatory cytokines were determined by using ELISA kits. Result: The results have shown that MSM could promote the cell proliferation and regulate the cell cycle arrest when EGFR inhibited (P less than 0.05). In addition, both MSM and EGFR-TKI could decrease the expressions of IL-1β, IL-6 and TNFα (P less than 0.05). These results showed that the beneficial roles of MSM in chicken IECs induced by LPS may be related to promoting cell proliferation and inhibiting inflammation; meanwhile, our experimental results may provide valuable information for further study of the possible mechanism of MSM to improve the chicken intestinal epithelial function by regulating EGFR signaling pathway.

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Effects of Glutamine on Growth Performance, Intestinal Morphology and Intestinal Barrier Function of Broilers

Cited by 4 publications

References 0 publications

Effects of Glutamine on the Mucosal Structure and Immune Cells in the Intestines of Broiler Chickens Challenged with Salmonella Enteritidis

Effects of Glutamine on the Mucosal Structure and Immune Cells in the Intestines of Broiler Chickens Challenged with Salmonella Enteritidis

Dietary Zn Deficiency Inhibits Cell Proliferation via the GPR39-Mediated Suppression of the PI3K/AKT/mTOR Signaling Pathway in the Jejunum of Broilers

Effects of Methylsulfonylmethane and Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor on Cell Proliferation, Cell Cycle and Anti-Inflammatory Effect in Chicken Intestinal Epithelial Cells with Lipopolysaccharide Challenge

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