Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm

Pelufo, Valeria; Armengol, María Fernanda López; Malcotti, V.; Venturino, Andrés; Aisen, E.G.

doi:10.1016/j.theriogenology.2014.09.007

Cited by 22 publications

(14 citation statements)

References 37 publications

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“…Post-thaw motility of ram semen freezing in breeding or non-breeding seasons with different extenders containing egg yolk ranged between 45.7% and 67.9% in many studies [7,14,15,28,32,39,40,42]. The result of our study shows that post-thaw motility in lyophilized group was in a good agreement with the findings of these studies [7,14,28,39,40,42].…”

Section: Discussionsupporting

confidence: 89%

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

et al. 2015

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Section: Discussionsupporting

confidence: 89%

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

et al. 2015

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“…Concerning morphology, the spermatozoa were classified into the following categories: (a) normal, (b) total abnormalities, (c) abnormal head, (d) abnormal midpiece and (e) abnormal tail. Furthermore, hypo‐osmotic swelling test (HOST) was performed to all samples according to Pelufo, López Armengol, Malcotti, Venturino, and Aisen () and the percentage of HOST‐positive spermatozoa (having coiled tails) was determined.…”

Section: Methodsmentioning

confidence: 99%

Effects of testicular hemodynamic and echogenicity changes on ram semen characteristics

Ntemka

Kiossis

Boscos

et al. 2018

Reprod Domestic Animals

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ContentsThis study investigated correlations among ram semen quality parameters, testicular vascularity and echotexture. Measurements were performed biweekly for a year, before (BE) and after ejaculation (AE), on seven Chios breed rams [three rams (group A) and four rams (group B) of 2-6 and 9-13 years old, respectively], considering breeding (1) and non-breeding period (2). Hemodynamics [Pulsatility Index (PI), Resistive Index (RI)] were evaluated in each testis. Images of testicular parenchyma were analysed to evaluate echogenicity [Mean Value (MV), Contrast (Con), Gray Value Distribution (GVD), Run Length Distribution (RunLD), Long Run Emphasis (LRunEm)].Semen volume (V), concentration (C), kinetics (CASA), morphology (Sperm Blue), viability (Eosin-Nigrosin) and DNA fragmentation (Acridine Orange) were assessed. In group A/2, semen V was correlated to left testis PI and right testis RI, BE (r = 0.4, p = 0.013/r = 0.6, p < 0.001, respectively), and to right testis PI and RI, AE (r = 0.5, p < 0.001). In A/1/AE, semen C was correlated to left testis PI (r = −0.5, p = 0.003). In group A/1/BE, RI of both testes was correlated to total and tail abnormalities (left: r = −0.5, p = 0.008/ p = 0.004, respectively -right: r = −0.4/r = −0.5, p = 0.01/ p = 0.008, respectively). In group B/1/BE, sperm tail and total abnormalities were correlated to left testis Con (r = 0.5, p = 0.02/ p < 0.001, respectively), whereas in A/2/BE, they were correlated to left testis LRunEm (r = −0.8/r = −0.7, p < 0.001, respectively). In group A/1/BE, DNA damage was correlated to right testis LRunEm (r = −0.6, p < 0.001) and tail abnormalities to left testis PI (r = −0.5, p = 0.03). In group B/1/AE, right testis LRunEm, GVD, RunLD, were correlated to sperm DNA integrity (r = −0.9, p < 0.001). Immotile spermatozoa and viability were correlated to left testis Con in group B/2/AE (r = −0.6/r = 0.6, p < 0.001) and to right testis MV in group A/1/ AE (r = −0.6/r = 0.6, p < 0.001). In conclusion, alterations in both ram testes blood flow and echotexture seem to be moderately correlated with sperm assessed parameters, depending on season and ram age. K E Y W O R D S Chios sheep, colour Doppler, ram semen, testicular echogenicity, testicular hemodynamics, ultrasonography | 51 NTEMKA ET Al. How to cite this article: Ntemka A, Kiossis E, Boscos C, Theodoridis A, Kourousekos G, Tsakmakidis I. Effects of testicular hemodynamic and echogenicity changes on ram semen characteristics. Reprod Dom Anim. 2018; 53(Suppl. 2):50-55. https://doi.

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“…Many studies have reported a decrease in sperm motility and the occurrence of lesions in the plasma membrane after cryopreservation. These phenomena represent a major source of damage to cryopreserved sperm (Pelufo et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

The in vitro investigation of lycopene effects on post-thawed ram sperm

et al. 2019

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This study was conducted to evaluate the effects of lycopene on quality parameters of cryopreserved ram semen. Semen samples were collected from three Santa Inês rams to form a seminal pool (n = 8). Aliquots from each pool were diluted in Tris-egg yolk extender to create experimental groups containing 0 µM (control), 0.1 µM, 1 µM and 5 µM lycopene. The samples were evaluated for sperm kinetics, integrity of plasma and acrosomal membranes, intracellular reactive oxygen species (ROS) production and lipid peroxidation immediately after thawing and after incubation for two hours at 37 °C. The addition of lycopene had no effect on the parameters that were evaluated at the time of thawing when compared with the control. However, after incubation, the groups with added lycopene showed a decrease in progressive motility. All experimental groups showed a significant reduction in linearity and straightness following incubation. Furthermore, the 5 µM lycopene group showed a decrease in wobble and an increase in amplitude of lateral head displacement. In conclusion, the addition of lycopene to the freezing extender of ram semen affected the kinetics parameters of cryopreserved spermatozoa after a two-hour incubation period.

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Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm

Cited by 22 publications

References 37 publications

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

Effects of testicular hemodynamic and echogenicity changes on ram semen characteristics

The in vitro investigation of lycopene effects on post-thawed ram sperm

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